Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction

Analytical Biochemistry

Volumn 424, Issue 2, 2012, Pages 124-126

  Ma, Rui ^a   Zhao, Jun ^b   Du, Hui Cong ^a   Tian, Shuang ^a,c   Li, Li Wen ^a  

^a NORTHWEST UNIVERSITY   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c CHINESE PLA GENERAL HOSPITAL   (China)

Author keywords

Endotoxin removing; Plasmid; Triton X 114

Indexed keywords

COST EFFECTIVENESS; DNA; ISOTHERMS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

COST EFFECTIVE STRATEGIES; ENDOTOXIN REMOVAL; ENDOTOXIN REMOVING; EXTRACTION METHOD; HEATING PROCESS; PLASMID; TEMPERATURE TRANSITION; TRITON X 114;

EXTRACTION;

DODECYL SULFATE SODIUM; ENDOTOXIN; PLASMID DNA; TRITON X 114;

ARTICLE; CONCENTRATION RESPONSE; EXTRACTION; INTERMETHOD COMPARISON; ISOTHERMAL EXTRACTION; NONHUMAN; PLASMID; PRIORITY JOURNAL;

EID: 84859034602     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.02.015     Document Type: Article

References (7)

1. M. Cotten, A. Baker, M. Saltik, E. Wagner, and M. Buschle Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus Gene Ther. 1 1994 239 246
2. I.P. Wicks, M.L. Howell, T. Hancock, H. Kohsaka, T. Olee, and D.A. Carson Bacterial lipopolysaccharide copurifies with plasmid DNA: implications for animal models and human gene therapy Hum. Gene Ther. 6 1995 317 323
3. J.F. Li, L. Li, and J. Sheen Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology Plant Methods 6 2010 1
4. S. Grimm, and F. Voss-Neudecker High-purity plasmid isolation using silica oxide Methods Mol. Biol. 235 2003 83 87
5. R. Lakshmi, V. Baskar, and U. Ranga Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix Anal. Biochem. 272 1999 109 112 (Pubitemid 29343390)
6. T. Gu, and P.A. Galera-Gómez Clouding of Triton X-114: The effect of added electrolytes on the cloud point of Triton X-114 in the presence of ionic surfactants Colloids Surf. A 104 1995 307 312
7. P. Mukherjee, S.K. Padhan, S. Dash, S. Patel, and B.K. Mishra Clouding behaviour in surfactant systems Adv. Colloid Interface Sci. 162 2011 59 79