Introducing site-specific glycosylation using protein engineering techniques reduces the immunogenicity of β-lactoglobulin

Bioscience, Biotechnology and Biochemistry

Volumn 76, Issue 3, 2012, Pages 478-485

  Tatsumi, Yoshiyuki ^a   Sasahara, Yoshimasa ^a   Kohyama, Noriki ^a   Ayano, Satomi ^a   Endo, Michio ^a   Yoshida, Tadashi ^a   Yamada, Kiyoshi ^b   Totsuka, Mamoru ^b   Hattori, Makoto ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

lactoglobulin; Functional improvement; Neoglycoconjugate; Protein conjugation; Protein engineering

Indexed keywords

ANTIBODY RESPONSE; CD SPECTRA; FUNCTIONAL IMPROVEMENT; GLYCOSYLATED; IMMUNOGENICITY; INTRINSIC FLUORESCENCE; LACTOGLOBULIN; METHYLOTROPHIC YEASTS; NEOGLYCOCONJUGATE; PICHIA PASTORIS; PROTEIN ENGINEERING; SDS-PAGE; SEQUENCE CODING; SIGNAL PEPTIDE; SITE-SPECIFIC; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; WILD TYPES;

BIOCHEMICAL ENGINEERING; ELECTROPHORESIS; ESTERIFICATION; GENETIC ENGINEERING; GLYCOSYLATION; MAMMALS; MONOCLONAL ANTIBODIES; SODIUM; SODIUM SULFATE;

YEAST;

LACTOGLOBULIN;

ANIMAL; ARTICLE; BINDING SITE; CATTLE; CHEMISTRY; ENZYME SPECIFICITY; FEMALE; GENETICS; GLYCOSYLATION; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOUSE; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

ANIMALS; BINDING SITES; CATTLE; FEMALE; GLYCOSYLATION; LACTOGLOBULINS; MICE; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

BOVINAE; MUS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 84859012499     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.110753     Document Type: Article

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