RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens

Histopathology

Volumn 60, Issue 5, 2012, Pages 804-815

  Masir, Noraidah ^a   Ghoddoosi, Mahdiieh ^a   Mansor, Suhada ^a   Abdul Rahman, Faridah ^a   Florence, Chandramaya S ^a   Mohamed Ismail, Nor Azlin ^a   Tamby, Mohammad Rafaee ^b   Md Latar, Nani Harlina ^a  

^a DEPARTMENT OF PHARMACOLOGY   (Malaysia)

^b UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Histopathological examination; RCL2; Tissue fixation

Indexed keywords

ALCOHOL; CYTOKERATIN ANTIBODY; FIXATIVE; FORMALDEHYDE; GENOMIC DNA; PROGESTERONE RECEPTOR; RCL2; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; FLUORESCENCE IN SITU HYBRIDIZATION; HISTOCHEMISTRY; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MICROTOMY; MORPHOLOGY; PRIORITY JOURNAL; SILVER STAINING; TISSUE FIXATION;

DNA; FEMALE; FIXATIVES; FORMALDEHYDE; GENOME; HUMANS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROTOMY; SILVER STAINING; TISSUE FIXATION;

EID: 84859007011     PISSN: 03090167     EISSN: 13652559     Source Type: Journal    
DOI: 10.1111/j.1365-2559.2011.04127.x     Document Type: Article

References (21)

1. Preusser M, Plumer S, Dirnberger E, Hainfellner JA, Mannhalter C. Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids. Brain Pathol. 2010; 20; 1010-1020.
2. Dotti I, Bonin S, Basili G et al. Effects of formalin, methacarn, and fineFIX fixatives on RNA preservation. Diagn. Mol. Pathol. 2010; 19; 112-122.
3. Eltoum I, Fredenburgh J, Myers RB, Grizzle WE. Introduction to the theory and practice of fixation of tissues. J. Histotechnol. 2001; 24; 173-190.
4. Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol. 2002; 161; 1961-1971.
5. Masuda N, Ohnishi T, Kawamoto S, Monden M, Okubo K. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res. 1999; 27; 4436-4443.
6. Belief V, Boissiere F, Bibeau F et al. Proteomic analysis of RCL2 paraffin-embedded tissues. J. Cell Mol. Med. 2008; 12; 2027-2036.
7. Stanta G, Mucelli SP, Petrera F, Bonin S, Bussolati G. A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues. Diagn. Mol. Pathol. 2006; 15; 115-123.
8. Buesa RJ. Histology without formalin? Ann. Diagn. Pathol. 2008; 12; 387-396.
9. Hall A, Harrington JM, Aw TC. Mortality study of British pathologists. Am. J. Ind. Med. 1991; 20; 83-89.
10. Beane Freeman LE, Blair A, Lubin JH et al. Mortality from lymphohematopoietic malignancies among workers in formaldehyde industries: the National Cancer Institute Cohort. J. Natl Cancer Inst. 2009; 101; 751-761.
11. Bussolati G, Chiusa L, Cimino A, D'Armento G. Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin. Virchows Arch. 2008; 452; 229-231.
12. Lassalle S, Hofman V, Marius I et al. Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives. Thyroid 2009; 19; 1239-1248.
13. International Agency for Research on Cancer (IARC/IAfRoC). Monograghs on the evaluation of carcinogenic risks to humans. Lyon, France: IARC, 2006.
14. ® non toxic, rormalin-free fixative for human and animal samples, user guide. 2008; 1-25.
15. Alphelys. formalin-free fixative. 2008; available online at.
16. Delfour C, Roger P, Bret C et al. RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. J. Mol. Diagn. 2006; 8; 157-169.
17. Denouel A, Boissiere-Michot F, Rochaix P, Bibeau F, Boulle N. An alternative fixative to formalin fixation for molecular applications: the RCL2((R))-CS100 approach. Methods Mol. Biol. 2011; 724; 297-307.
18. van Essen HF, Verdaasdonk MA, Elshof SM, de Weger RA, van Diest PJ. Alcohol based tissue fixation as an alternative for formaldehyde: influence on immunohistochemistry. J. Clin. Pathol. 2010; 63; 1090-1094.
19. Hewitt SM, Lewis FA, Cao Y et al. Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue. Arch. Pathol. Lab. Med. 2008; 132; 1929-1935.
20. Ventura RA, Martin-Subero JI, Jones M et al. FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue. J. Mol. Diagn. 2006; 8; 141-151.
21. Titford ME, Horenstein MG. Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch. Pathol. Lab. Med. 2005; 129; 502-506.