Myofibroblast differentiation modulates keratocyte crystallin protein expression, concentration, and cellular light scattering

Investigative Ophthalmology and Visual Science

Volumn 53, Issue 2, 2012, Pages 770-778

  Jester, James V ^a   Brown, Donald ^a   Pappa, Aglaia ^b,c   Vasiliou, Vasilis ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF COLORADO   (United States)

^c DEMOCRITUS UNIVERSITY OF THRACE   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDE DEHYDROGENASE; ALDEHYDE DEHYDROGENASE ISOENZYME 1; ALPHA SMOOTH MUSCLE ACTIN; CRYSTALLIN; KERATOCAN; LUMICAN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL VOLUME; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CORNEA; CORNEA CELL; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; LIGHT SCATTERING; MICROSCOPY; MYOFIBROBLAST; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; RABBIT; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; CELL CULTURE; COMPARATIVE STUDY; CORNEA STROMA; CULTURE MEDIUM; CYTOLOGY; GENETICS; LIGHT; METABOLISM; RADIATION SCATTERING;

ANIMALS; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELLS, CULTURED; CORNEAL KERATOCYTES; CORNEAL STROMA; CRYSTALLINS; CULTURE MEDIA, SERUM-FREE; LIGHT; MICROSCOPY, CONFOCAL; MYOFIBROBLASTS; RABBITS; REAL-TIME POLYMERASE CHAIN REACTION; SCATTERING, RADIATION;

EID: 84858951956     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.11-9092     Document Type: Article

References (45)

1. Jester JV, Barry-Lane PA, Cavanagh HD, Petroll WM. Induction of alpha-smooth muscle actin expression and myofibroblast transformation in cultured corneal keratocytes. Cornea. 1996;15:505-516.
2. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci U S A. 1996;93:4219-4223.
3. Berryhill BL, Kader R, Kane B, Birk DE, Feng J, Hassell JR. Partial restoration of the keratocyte phenotype to bovine keratocytes made fibroblastic by serum. Invest Ophthalmol Vis Sci. 2002;43: 3416-3421.
4. Jester JV, Ho-Chang J. Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction. Exp Eye Res. 2003;77: 581-592.
5. Jester JV, Huang J, Fisher S, et al. Myofibroblast differentiation of normal human keratocytes and hTERT, extended-life human corneal fibroblasts. Invest Ophthalmol Vis Sci. 2003;44:1850-1858.
6. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986; 103:2787-2796.
7. Petroll WM, Cavanagh HD, Barry P, Andrews P, Jester JV. Quantitative analysis of stress fiber orientation during corneal wound contraction. J Cell Sci. 1993;104:353-363.
8. Grinnell F, Petroll WM. Cell motility and mechanics in threedimensional collagen matrices. Annu Rev Cell Dev Biol. 2010;26: 335-361.
9. Jester JV, Petroll WM, Cavanagh HD. Corneal stromal wound healing in refractive surgery: the role of myofibroblasts. Prog Retin EyeRes. 1999;18:311-356.
10. Moller-Pedersen T, Cavanagh HD, Petroll WM, Jester JV. Neutralizing antibody to TGFbeta modulates stromal fibrosis but not regression of photoablative effect following PRK. Curr Eye Res. 1998;17:736-747.
11. Jester JV, Barry-Lane PA, Petroll WM, Olsen DR, Cavanagh HD. Inhibition of corneal fibrosis by topical application of blocking antibodies to TGF beta in the rabbit. Cornea. 1997;16:177-187.
12. Cintron C, Szamier RB, Hassinger LC, Kublin CL. Scanning electron microscopy of rabbit corneal scars. Invest Ophthalmol Vis Sci. 1982;23:50-63.
13. Cintron C, Hong BS, Kublin CL. Quantitative analysis of collagen from normal developing corneas and corneal scars. Curr Eye Res. 1981;1:1-8.
14. Rawe IM, Meek KM, Leonard DW, Takahashi T, Cintron C. Structure of corneal scar tissue: an X-ray diffraction study. Biophys J. 1994;67:1743-1748.
15. Lipshitz I, Loewenstein A, Varssano D, Lazar M. Late onset corneal haze after photorefractive keratectomy for moderate and high myopia. Ophthalmology. 1993;104:369-373. discussion 373-364.
16. Shii D, Nakagawa S, Shinomiya K, et al. Cyclosporin A eye drops inhibit fibrosis and inflammatory cell infiltration in murine type I allergic conjunctivitis without affecting the early-phase reaction. Curr Eye Res. 2009;34:426-437.
17. Nien CJ, Flynn KJ, Chang M, Brown D, Jester JV. Reducing peak corneal haze after photorefractive keratectomy in rabbits: prednisolone acetate 1.00% versus cyclosporine A 0.05%. J Cataract Refract Surg. 2011;37:937-944.
18. Jester JV, Møller-Pedersen T, Huang J, et al. The cellular basis of corneal transparency: evidence for 'corneal crystallins'. J Cell Sci. 1999;112(Pt 5):613-622.
19. Alexander RJ, Silverman B, Henley WL. Isolation and characterization of BCP 54, the major water soluble protein of bovine cornea. Exp Eye Res. 1981;32:205-216.
20. Holt WS, Kinoshita JH. The soluble proteins of the bovine cornea. Invest Ophthalmol Vis Sci. 1973;12:114-126.
21. Jester JV. Corneal crystallins and the development of cellular transparency. Semin Cell Dev Biol. 2008;19:82-93.
22. Jester JV, Budge A, Fisher S, Huang J. Corneal keratocytes: phenotypic and species differences in abundant protein expression andin vitro light scattering. Invest Ophthalmol Vis Sci. 2005;46:2369-2378.
23. Piatigorsky J. Enigma of the abundant water-soluble cytoplasmic proteins of the cornea: the "refraction" hypothesis. Cornea. 2001; 20:853-858.
24. Jester JV, Lee YG, Huang J, et al. Development of postnatal corneal transparency is associated with keratocyte cell cycle exit and marked expression of ALDH1A1. Invest Ophthalmol Vis Sci. 2007; 48:4061-4069.
25. Sax CM, Kays WT, Salamon C, Chervenak MM, Xu YS, Piatigorsky J. Transketolase gene expression in the cornea is influenced by environmental factors and developmentally controlled events. Cornea. 2000;19:833-841.
26. Pei Y, Reins RY, McDermott AM. Aldehyde dehydrogenase (ALDH) 3A1 expression by the human keratocyte and its repair phenotypes. Exp Eye Res. 2006;83:1063-1073.
27. Møller-Pedersen T. Keratocyte reflectivity and corneal haze. Exp Eye Res. 2004;78:553-560.
28. Møller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV. Confocal microscopic characterization of wound repair after photorefractive keratectomy. Invest Ophthalmol Vis Sci. 1998;39: 487-501.
29. Brown DJ, Lin B, Holguin B. Expression of neuregulin 1, a member of the epidermal growth factor family, is expressed as multiple splice variants in the adult human cornea. Invest Ophthalmol Vis Sci. 2004;45:3021-3029.
30. Spirin KS, Ljubimov AV, Castellon R, et al. Analysis of gene expression in human bullous keratopathy corneas containing limiting amounts of RNA. Invest Ophthalmol Vis Sci. 1999;40:3108-3115.
31. Barry-Lane PA, Wilson SE, Cavanagh HD, Petroll WM, Jester JV. Characterization of SV40-transfected cell strains from rabbit keratocytes. Cornea. 1997;16:72-78.
32. Lassen N, Pappa A, Black WJ, et al. Antioxidant function of corneal ALDH3A1 in cultured stromal fibroblasts. Free Radic Biol Med. 2006;41:1459-1469.
33. Stagos D, Chen Y, Cantore M, Jester JV, Vasiliou V. Corneal aldehyde dehydrogenases: multiple functions and novel nuclear localization. Brain Res Bull. 2010;81:211-218.
34. Beales MP, Funderburgh JL, Jester JV, Hassell JR. Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture. Invest Ophthalmol Vis Sci. 1999;40:1658-1663.
35. Karring H, Thogersen IB, Klintworth GK, Enghild JJ, Møller-Pedersen T. Proteomic analysis of the soluble fraction from human corneal fibroblasts with reference to ocular transparency. Mol Cell Proteomics. 2004;3:660-674.
36. Stramer BM, Cook JR, Fini ME, Taylor A, Obin M. Induction of the ubiquitin-proteasome pathway during the keratocyte transition to the repair fibroblast phenotype. Invest Ophthalmol Vis Sci. 2001; 42:1698-1706.
37. Stramer BM, Fini ME. Uncoupling keratocyte loss of corneal crystallin from markers of fibrotic repair. Invest Ophthalmol Vis Sci. 2004;45:4010-4015.
38. Lassen N, Black WJ, Estey T, Vasiliou V. The role of corneal crystallins in the cellular defense mechanisms against oxidative stress. Semin Cell Dev Biol. 2008;19:100-112.
39. Manzer R, Pappa A, Estey T, Sladek N, Carpenter JF, Vasiliou V. Ultraviolet radiation decreases expression and induces aggregation of corneal ALDH3A1. Chem Biol Interact. 2003;143-144:45-53.
40. Manzer R, Qamar L, Estey T, Pappa A, Petersen DR, Vasiliou V. Molecular cloning and baculovirus expression of the rabbit corneal aldehyde dehydrogenase (ALDH1A1) cDNA. DNA Cell Biol. 2003; 22:329-338.
41. Pappa A, Brown D, Koutalos Y, DeGregori J, White C, Vasiliou V. Human aldehyde dehydrogenase 3A1 inhibits proliferation and promotes survival of human corneal epithelial cells. J Biol Chem. 2005;280:27998-28006.
42. Estey T, Cantore M, Weston PA, Carpenter JF, Petrash JM, Vasiliou V. Mechanisms involved in the protection of UV-induced protein inactivation by the corneal crystallin ALDH3A1. J Biol Chem. 2007;282:4382-4392.
43. Bloemendal H, de Jong W, Jaenicke R, Lubsen NH, Slingsby C, Tardieu A. Ageing and vision: structure, stability and function of lens crystallins. Prog Biophys Mol Biol. 2004;86:407-485.
44. Lassen N, Bateman JB, Estey T, et al. Multiple and additive functions of ALDH3A1 and ALDH1A1: cataract phenotype and ocular oxidative damage in aldh3a1(-/-)/aldh1a1(-/-) knockout mice. J Biol Chem. 2007;282:25668-25676.
45. Chen Y, Koppaka V, Thompson DC, Vasiliou V. Focus on Molecules: ALDH1A1: From lens and corneal crystallin to stem cell marker. Exp Eye Res. Published online April 23, 2011.