Flat-top illumination profile in an epifluorescence microscope by dual microlens arrays

Cytometry Part A

Volumn 81 A, Issue 4, 2012, Pages 324-331

  Coumans, Frank A W ^a   van der Pol, Edwin ^b   Terstappen, Leon W M M ^a  

^a UNIVERSITY OF TWENTE   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Beam shaping; Coefficient of variation; Epifluorescence microscopy; Flat top; Homogeneous illumination profile; Microlens array

Indexed keywords

ARTICLE; CALIBRATION; EQUIPMENT DESIGN; FLUORESCENCE MICROSCOPY; ILLUMINATION; IMAGE ENHANCEMENT; INSTRUMENTATION; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

CALIBRATION; EQUIPMENT DESIGN; IMAGE ENHANCEMENT; LIGHTING; MICROSCOPY, FLUORESCENCE; SENSITIVITY AND SPECIFICITY;

EID: 84858799667     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22029     Document Type: Article

References (20)

1. Florijn RJ, Bonnet J, Vrolijk H, Raap AK, Tanke HJ. Effect of chromatic errors in microscopy on the visualisation of multi-color fluorescence in situ hybridization. Cytometry 1996; 23: 8-14.
2. Wang N, Pan Y, Heiden T, Tribukait B. Fluorescence image cytometry for measurement of nuclear DNA content in surgical pathology. Cytometry 1995; 22: 323-329.
3. Model MA, Burkhardt JK. A standard for calibration and shading correction of a fluorescence microscope. Cytometry 2001; 44: 309-316.
4. Klein AD, van den Doel R, Young IT, Ellenberger SL, van Vliet LJ. Quantitative evaluation and comparison of light microscopes. In: Farkas DL, Leif RC, Tromberg BJ, editors. Optical Investigation of Cells In Vitro and In Vivo. Proc SPIE. Prog Biomed Opt 1998; 3260: 162-173.
5. Bright GR, Fisher GW, Rogowska J, Taylor DL. Fluorescence ratio imaging microscopy: Temporal and spatial measurements of cytoplasmic pH. J Cell Biol 1987; 104: 1019-1033.
6. Ji JK, Kwon YS. Conical microlens arrays that flatten optical-irradiance profiles of nonuniform sources. Appl Opt 1995; 34: 2841-2843.
7. Scholtens TM, Schreuder F, Ligthart ST, Swennenhuis J, Tibbe AGT, Greve J, Terstappen LWMM. Cell tracks TDI: An image cytometer for cell characterization. Cytometry A 2011; 79A: 203-213.
8. Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, Tibbe A, Uhr JW, Terstappen LWMM. Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with non-malignant diseases. Clin Cancer Res 2004; 10: 6897-6904.
9. de Bono JS, Attard G, Adjei A, Pollak MN, Fong PC, Haluska P, Roberts L, Melvin C, Repollet M, Chianese D, et al. Potential applications for circulating tumor cells expressing the insulin growth factor-I receptor. Clin Cancer Res 2007; 13: 3611-3616.
10. Riethdorf S, Müller V, Zhang L, Loibl S, Komor M, Roller M, Huober J, Fehm T, Schrader I, Hilfrich J, Holms F, Tesch H, Eidtmann H, Untch M, von Minckwitz G, Pantel K. Detection and HER2 expression of circulating tumor cells: Prospective monitoring in breast cancer patients treated in the neoadjuvant geparquattro trial. Clin Cancer Res 2010; 16: 2634-2645.
11. van der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG, Nieuwland R. Optical and non-optical methods for detection and characterisation of microparticles and exosomes. J Thromb Haemostasis 2010; 8: 2596-2607.
12. Voelkel R, Weible KJ. Laser beam homogenizing: Limitations and constraints. In: Duparré A, Geyl R, editors. SPIE Conference proceedings vol 2008; 7102. Bellingham, WA: SPIE Publications; Abstract 71020J.
13. Wright D, Greve P, Fleischer J, Austin L. Laser beam width, divergence and beam propagation factor: An international standardization approach. Opt Quantum Electron 1992; 24: S993-S1000.
14. Büttner A, Zeitner UD. Wave optical analysis of light emitting diode beam shaping using micro lens arrays. Opt Eng 2002; 41: 2393-2401.
15. Tibbe AGJ, de Grooth BG, Greve J, Dolan GJ, Rao C, Terstappen LWMM. Magnetic field design for selecting and aligning immunomagnetic labeled cells. Cytometry 2002; 47: 163-172.
16. Maecker HT, Trotter J. Flow cytometry controls, instrument setup and the determination of positivity. Cytometry Part A 2006; 69A: 1037-1042.
17. Hoffnagle JA, Jefferson CM. Design and performance of a refractive optical system that converts a Gaussian to a flattop beam. Appl Opt 2000; 39: 5488-5499.
18. Nemoto K, Fujii T, Goto N, Takino H, Kobayashi T, Shibata N, Yamamura K, Mori Y. Laser beam intensity profile transformation with a fabricated mirror. Appl Opt 1997; 36: 551-557.
19. Chang SP, Kuo JM, Lee YP, Lu CM, Ling KJ. Transformation of Gaussian to coherent uniform beams by inverse-Gaussian transmittive filters. Appl Opt 1998; 37: 747-752.
20. Sales TRM. Structured microlens arrays for beam shaping. Opt Eng 2003; 42: 3084-3085.