Enhanced labeling of microalgae cellular lipids by application of an electric field generated by alternating current

Bioresource Technology

Volumn 111, Issue , 2012, Pages 323-327

  Su, Li Chien ^a   Hsu, Yi Hsiang ^a   Wang, Hsiang Yu ^a  

^a NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Electroporation; Lipid quantification; Microalgae

Indexed keywords

ALTERNATING CURRENT; CELLULAR LIPID; CHLORELLA VULGARIS; DECAY RATE; ELECTROPORATION; FLUORESCENCE DETECTION; FLUORESCENCE INTENSITIES; FLUORESCENT LABELING; INTENSITY FLUCTUATIONS; INTENSITY VARIATIONS; MICRO-ALGAE; NILE RED; SIGNAL INTENSITIES; SPIRULINA SP;

DECAY (ORGANIC); ELECTRIC FIELDS; FLUORESCENCE; LIPIDS; MICROORGANISMS;

ALGAE;

LIPID;

ELECTRICAL PROPERTY; ELECTRICITY GENERATION; FLUORESCENCE; LIPID; MICROALGA;

ALTERNATING CURRENT; ARTICLE; CHLORELLA VULGARIS; CONTROLLED STUDY; ELECTRIC FIELD; FLUORESCENCE; FUNGAL CELL; MICROALGA; NONHUMAN; PRIORITY JOURNAL; SPIRULINA;

ELECTRICITY; FLUORESCENCE; MICROALGAE;

CHLORELLA VULGARIS; SPIRULINA SP.;

EID: 84858754087     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.01.180     Document Type: Article

References (18)

1. Ahmad A.L., Yasin N.H.M., Derek C.J.C., Lim J.K. Microalgae as a sustainable energy source for biodiesel production: a review. Renew. Sust. Energy Rev. 2011, 15(1):584-593.
2. Bligh E.G. Citation classic-rapid method of total lipid extraction and purification. Curr. Contents 1978, 52:11.
3. Bustard M.T., Tryfona T. Enhancement of biomolecule transport by electroporation: a review of theory and practical application to transformation of Corynebacterium glutamicum. Biotechnol. Bioeng. 2006, 93(3):413-423.
4. Chang J.S., Chen C.Y., Yeh K.L., Aisyah R., Lee D.J. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresour. Technol. 2011, 102(1):71-81.
5. Chang J.S., Chen C.Y., Yeh K.L., Su H.M., Lo Y.C., Chen W.M. Strategies to enhance cell growth and achieve high-level oil production of a Chlorella vulgaris isolate. Biotechnol. Prog. 2010, 26(3):679-686.
6. Chow K.C., Tung W.L. Electrotransformation of Chlorella vulgaris. Plant Cell Rep. 1999, 18(9):778-780.
7. Fox M.B., Esveld D.C., Valero A., Luttge R., Mastwijk H.C., Bartels P.V., van den Berg A., Boom R.M. Electroporation of cells in microfluidic devices: a review. Anal. Bioanal. Chem. 2006, 385(3):474-485.
8. Ho S.Y., Mittal G.S. Electroporation of cell membranes: a review. Crit. Rev. Biotechnol. 1996, 16(4):349-362.
9. Hu Q., Chen W., Zhang C.W., Song L.R., Sommerfeld M. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J. Microbiol. Meth. 2009, 77(1):41-47.
10. Hu Q.A., Chen W., Sommerfeld M. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresour. Technol. 2011, 102(1):135-141.
11. Neumann E., Schaeferridder M., Wang Y., Hofschneider P.H. Gene-transfer into mouse lyoma cells by electroporation in high electric-fields. EMBO J. 1982, 1(7):841-845.
12. Prausnitz M.R., Azencott H.R., Peter G.F. Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonication. Ultrasound Med. Biol. 2007, 33(11):1805-1817.
13. Priscu J.C., Priscu L.R., Palmisano A.C., Sullivan C.W. Estimation of neutral lipid-levels in Antarctic sea ice microalgae by Nile red fluorescence. Antarct. Sci. 1990, 2(2):149-155.
14. Raleigh B., Elsey D., Jameson D., Cooney M.J. Fluorescent measurement of microalgal neutral lipids. J. Microbiol. Meth. 2007, 68(3):639-642.
15. Schmitz G., Grandl M. fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and ox-LDL-generated phospholipidosis in human macrophages. Cytometry A 2010, 77A(3):231-242.
16. Singh A., Olsen S.I. A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels. Appl. Energy 2011, 88(10):3548-3555.
17. Tsong T. Electroporation of cell membranes. Biophys. J. 1991, 60(2):297-306.
18. Vargas J.V.C., Satyanarayana K.G., Mariano A.B. A review on microalgae, a versatile source for sustainable energy and materials. Int. J. Energy Res. 2011, 35(4):291-311.