Xylem sap collection and extraction methodologies to determine in vivo concentrations of ABA and its bound forms by gas chromatography-mass spectrometry (GC-MS)

Plant Methods

Volumn 8, Issue 1, 2012, Pages

  Netting, Andrew G ^a   Theobald, Julian C ^b,c   Dodd, Ian C ^c  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b PernodRicard New Zealand ^*  (New Zealand)

^c LANCASTER UNIVERSITY   (United Kingdom)

Author keywords

ABA; ABA GE; Flacca; GC MS; Mutant; Notabilis; Sitiens; Tomato; Xylem sap

Indexed keywords

LYCOPERSICON ESCULENTUM;

EID: 84858738626     PISSN: None     EISSN: 17464811     Source Type: Journal    
DOI: 10.1186/1746-4811-8-11     Document Type: Article

References (34)

1. Dodd IC. Root-to-shoot signalling: assessing the roles of "up" in the up and down world of long-distance signalling in planta. Plant Soil 2005, 274:251-270.
2. Jiang F, Hartung W. Long-distance signalling of abscisic acid (ABA): the factors regulating the intensity of the ABA signal. J Exp Bot 2008, 59:37-43.
3. Holbrook NM, Shashidhar VR, James RA, Munns R. Stomatal control in tomato with ABA-deficient roots: response of grafted plants to soil drying. J Exp Bot 2002, 53:1503-1514. 10.1093/jexbot/53.373.1503, 12021298.
4. Chen G, Lips SH, Sagi M. Biomass production, transpiration rate and endogenous abscisic acid levels in grafts of flacca and wild-type tomato (Lycopersicon esculentum). Funct Plant Biol 2002, 29:1329-1335.
5. Dodd IC, Theobald JC, Richer SK, Davies WJ. Partial phenotypic reversion of ABA-deficient flacca tomato (Solanum lycopersicum) scions by a wild-type rootstock: normalising shoot ethylene relations promotes leaf area but does not diminish whole plant transpiration rate. J Exp Bot 2009, 60:4029-4039. 10.1093/jxb/erp236, 2755025, 19648172.
6. Netting AG, Windsor ML, Milborrow BV. The isolation and identification of the prosthetic group released from a bound form of abscisic acid. Plant Growth Regulation 1992, 11:327-334.
7. Netting AG, Windsor ML, Milborrow BV. Endogenous biosynthetic precursors of (+)-abscisic acid. 3. Incorporation of H2 from (H2O)-H-2 and O-18 from O-18(2) into precursors. Aust J Plant Physiol 1997, 24:175-184.
8. Taylor IB, Sonneveld T, Bugg TDH, Thompson AJ. Regulation and manipulation of the biosynthesis of abscisic acid, including the supply of xanthophyll precursors. J Plant Growth Regul 2005, 24:253-273.
9. Burbidge A, Grieve TM, Jackson A, Thompson A, McCarty DR, Taylor IB. Characterization of the ABA-deficient tomato mutant notabilis and its relationship with maize Vp14. Plant J 1999, 17:427-431. 10.1046/j.1365-313X.1999.00386.x, 10205899.
10. Harrison E, Burbidge A, Okyere JP, Thompson AJ, Taylor IB. Identification of the tomato ABA-deficient mutant sitiens as a member of the ABA-aldehyde oxidase gene family using genetic and genomic analysis. Plant Growth Regul 2011, 64:301-309.
11. Sauter A, Dietz K-J, Hartung W. A possible stress physiological role of abscisic acid conjugates in root-to-shoot signalling. Plant Cell Environ 2002, 25:223-228. 10.1046/j.1365-3040.2002.00747.x, 11841665.
12. Dietz KJ, Wichert K, Sauter A, Messdaghi D, Hartung W. Characterisation of an extracellular B-glucosidase in barley involved in the hydrolysis of ABA glucose conjugate in leaves. J Exp Bot 2000, 51:937-944. 10.1093/jexbot/51.346.937, 10948220.
13. Else MA, Taylor JM, Atkinson CJ. Anti-transpirant activity in xylem sap from flooded tomato (Lycopersicon esculentum Mill.) plants is not due to pH-mediated redistributions of root- or shoot-sourced ABA. J Exp Bot 2006, 57:3349-3357. 10.1093/jxb/erl099, 16940038.
14. Dodd IC. Measuring and modeling xylem ABA concentration ([X-ABA]) in tomato plants exposed to deficit irrigation (DI) and partial rootzone drying (PRD). Acta Horticulturae 2008, 792:225-231.
15. Dodd IC, Egea G, Watts C, Whalley WR. Root water potential integrates discrete soil physical properties to influence ABA signalling during partial rootzone drying. J Exp Bot 2010, 61:3543-3551. 10.1093/jxb/erq195, 20591896.
16. Plauborg F, Abrahamsen P, Gjettermann B, Mollerup M, Iversen BV, Liu F, Andersen MN, Hansen S. Modelling of root ABA synthesis, stomatal conductance, transpiration and potato production under water saving irrigation regimes. Agr Water Manag 2010, 98:425-439.
17. Taylor IB, Tarr AR. Phenotypic interactions between abscisic acid deficient tomato mutants. Theor Appl Genet 1984, 68:115-119.
18. Dodd IC. Soil moisture heterogeneity during deficit irrigation alters root-to-shoot signalling of abscisic acid. Funct Plant Biol 2007, 34:439-448.
19. Else MA, Davies WJ, Whitford PN, Hall KC, Jackson MB. Concentrations of abscisic acid and other solutes in xylem sap from root systems of tomato and castor-oil plants are distorted by wounding and variable sap flow rates. J Exp Bot 1994, 45:313-323.
20. Jachetta JJ, Appleby AP, Boersma L. Use of the pressure-vessel to measure concentrations of solutes in apoplastic and membrane-filtered symplastic sap in sunflower leaves. Plant Physiol 1986, 82:995-999. 10.1104/pp.82.4.995, 1056247, 16665180.
21. Jokhan AD, Harink RJ, Jackson MB. Concentration and delivery of abscisic acid in xylem sap are greater at the shoot base than at a target leaf nearer to the shoot apex. Plant Biology 1999, 1:253-260.
22. Li B, Feng Z, Xie M, Sun M, Zhao Y, Liang L, Liu G, Zhang J, Jia W. Modulation of the root-sourced ABA signal along its way to the shoot in Vitis riparia × Vitis labrusca under water deficit. J Exp Bot 2011, 62:1731-1741. 10.1093/jxb/erq390, 21131549.
23. Schurr U. Xylem sap sampling - new approaches to an old topic. Trends Plant Sci 1997, 3:293-298.
24. Tiekstra AE, Else MA, Jackson MB. External pressures based on leaf water potentials do not induce xylem sap to flow at rates of whole plant transpiration from roots of flooded or well-drained tomato and maize plants. Impact of shoot hydraulic resistances. Ann Bot 2000, 86:665-674.
25. Chen G, Fu XP, Lips SH, Sagi M. Control of plant growth resides in the shoot, and not in the root, in reciprocal grafts of flacca and wild-type tomato (Lycopersicon esculentum) in the presence and absence of salinity stress. Plant Soil 2003, 256:205-215.
26. Neill SJ, Horgan R. Abscisic acid production and water relations in wilty tomato mutants subjected to water deficiency. J Exp Bot 1985, 36:1222-1231.
27. Jones HG, Sharp CS, Higgs KH. Growth and water relations of wilty mutants of tomato (Lycopersicon esculentum Mill.). J Exp Bot 1987, 38:1848-1856.
28. Cornish K, Zeevaart JAD. Phenotypic expression of wild-type tomato and three wilty mutants in relation to abscisic acid accumulation in roots and leaflets of reciprocal grafts. Plant Physiol 1988, 87:190-194. 10.1104/pp.87.1.190, 1054723, 16666101.
29. Mulholland BJ, Hussain A, Black CR, Taylor IB, Roberts JA. Does root-sourced ABA have a role in mediating growth and stomatal responses to soil compaction in tomato (Lycopersicon esculentum)?. Physiol Plant 1999, 107:267-276.
30. Sagi M, Fluhr R, Lips SH. Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype. Plant Physiol 1999, 120:571-577. 10.1104/pp.120.2.571, 59296, 10364409.
31. Mulholland BJ, Thompson AJ, Jackson AC, Taylor IB, McKee JMT. Can ABA mediate salinity root stress in tomato?. Environ Exp Bot 2003, 50:17-28.
32. Linforth RST, Bowman WR, Griffin DA, Marples BA, Taylor IB. 2-trans-ABA alcohol accumulation in the wilty tomato mutants flacca and sitiens. Plant Cell Environ 1987, 10:599-606.
33. Duffield PH, Netting AG. Methods for the quantitation of abscisic acid and its precursors from plant tissues. Anal Biochem 2001, 289:251-259. 10.1006/abio.2000.4943, 11161319.
34. Quarrie SA, Whitford PN, Appleford NEJ, Wang TL, Cook SK, Henson IE, Loveys BR. A monoclonal antibody to (S)-abscisic acid: its characterisation and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves. Planta 1988, 173:330-339.