Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1

FEBS Letters

Volumn 586, Issue 6, 2012, Pages 675-679

  Varsaki, Athanasia ^a   Lamb, Heather K ^b   Eleftheriadou, Olga ^a   Vandera, Elpiniki ^a   Thompson, Paul ^b   Moncalián, Gabriel ^c   De La Cruz, Fernando ^c   Hawkins, Alastair R ^b   Drainas, Constantin ^a  

^a UNIVERSITY OF IOANNINA   (Greece)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF CANTABRIA   (Spain)

Author keywords

Accessory protein; Bacterial conjugation; ColE1 plasmid; Plasmid mobilization; Protein interaction; Relaxase

Indexed keywords

BACTERIAL PROTEIN; PROTEIN MBEA; PROTEIN MBEC; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; IN VIVO STUDY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; TWO HYBRID SYSTEM;

BACTERIAL PROTEINS; BASE SEQUENCE; CONJUGATION, GENETIC; DNA, SINGLE-STRANDED; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PLASMIDS; TWO-HYBRID SYSTEM TECHNIQUES;

BACTERIA (MICROORGANISMS);

EID: 84858706207     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2012.01.060     Document Type: Article

References (35)

1. van Rensburg, A.J. and Hugo, N. (1969) Characterization of DNA of colicinogenic factor E1 in a providence strain. J. Gen. Microbiol. 58 (3), 421-422.
2. Cabezón, E., Sastre, I.J. and de la Cruz, F. (1997) Genetic evidence of a coupling role of the TraG protein family in bacterial conjugation. Mol. Gen. Genet. 254, 400-406. (Pubitemid 127511345)
3. Clewell, D.B. and Helinski, D.R. (1969) Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. USA 62, 1159-1166.
4. Garcillán-Barcia, M.P., Francia, M.V. and de la Cruz, F. (2009) The diversity of the conjugative relaxases and its application in plasmid classification. FEMS Microbiol. Rev. 33 (3), 657-687.
5. Boyd, A.C., Archer, J.A.K. and Sherratt, D.J. (1989) Characterization of the ColE1 mobilization region and its protein products. Mol. Gen. Genet. 217, 488-498.
6. Varsaki, A., Lucas, M., Afendra, A.S., Drainas, C. and de la Cruz, F. (2003) Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilization. Mol. Microbiol. 48, 481-493. (Pubitemid 36819083)
7. Varsaki, A., Moncallian, G., Garcillan-Barcia, M.P., Drainas, C. and de la Cruz, F. (2009) Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins. J. Bacteriol. 191, 1446-1455.
8. Lovett, M.A. and Helinski, D.R. (1975) Relaxation complexes of plasmid DNA and proteins II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1. J. Biol. Chem. 250, 8790-8795.
9. Yamada, Y., Yamada, M. and Nakazawa, A. (1995) A ColE1-encoded gene directs entry exclusion of the plasmid. J. Bacteriol. 177, 6064-6068.
10. Alvarez-Martinez, C.E. and Christie, P.J. (2009) Biological diversity of prokaryotic type IV secretion systems. Microbiol. Mol. Biol. Rev. 73, 775-808.
11. de la Cruz, F., Frost, L.S., Meyer, R.J. and Zechner, E.L. (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol. Rev. 34, 18-40.
12. Zhang, S. and Meyer, R. (1997) The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer. Mol. Microbiol. 25, 509-516. (Pubitemid 27373579)
13. Bowie, J.U. and Sauer, R.T. (1990) TraY proteins of F and related episomes are members of the Arc and Mnt repressor family. J. Mol. Biol. 211, 5-6.
14. Wong, J.J., Lu, J., Edwards, R.A., Frost, L.S. and Glover, J.N. (2011) Structural basis of cooperative DNA recognition by the plasmid conjugation factor. TraM. Nucl. Acids Res. 39 (15), 6775-6788.
15. Moncalián, G. and de la Cruz, F. (2004) DNA binding properties of protein TrwA, a possible structural variant of the Arc represor superfamily. Biochim. Biophys. Acta 1701, 15-23. (Pubitemid 39140782)
16. Yoshida, H., Furuya, N., Lin, Y.J., Guntert, P., Romano, T. and Kainoshom, M. (2008) Structural basis of the role of the NikA ribbon-helix-helix domain in initiating bacterial conjugation. J. Mol. Biol. 384, 690-701.
17. Ziegelin, G., Furste, J.P. and Lanka, E. (1989) TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin. J. Biol. Chem. 264, 11989-11994. (Pubitemid 19185373)
18. Scherzinger, E., Lurz, R., Otto, S. and Dobrinski, B. (1992) In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins. Nucleic Acids Res. 20 (1), 41-48.
19. Chen, Y., Staddon, J.H. and Dunny, G.M. (2007) Specificity determinants of conjugative DNA processing in Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01. Mol. Microbiol. 63, 1549-1564.
20. Schröder, G., Krause, S., Zechner, E., Traxler, B., Yeo, H.J., Lurz, R., Waksman, G. and Lanka, E. (2002) TraG-like proteins of DNA transfer systems and of the Helicobacter pilori type IV secretion system: Inner membrane gate for exported substrates? J. Bacteriol. 184 (10), 2767-2779. (Pubitemid 34457044)
21. Llosa, M., Zunzunegui, S. and de la Cruz, F. (2003) Conjugative coupling proteins interact with the cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes. Proc. Natl. Acad. Sci. USA 100 (8), 10465-10470. (Pubitemid 37071901)
22. Abajy, M.A., Kopeć, J., Schiwon, K., Burzynski, M., Do{combining double acute accent}ring, M., Bohn, C. and Grohmann, E. (2007) A type IV-secretion-like system is required for conjugative DNA transport of broad-host-range plasmid pIP501 in Grampositive bacteria. J. Bacteriol. 189, 2487-2496. (Pubitemid 46411363)
23. Tato, I., Matilla, I., Arechaga, I., Zunzunegui, S., de la Cruz, F. and Cabezón, E. (2007) The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA. Implications for a common assembly mechanism of DNA translocating motors. J. Biol. Chem. 282 (35), 25569-25576. (Pubitemid 47372773)
24. Chen, Y., Zhang, X., Manias, D., Yeo, H.J., Dunny, G.M. and Christie, P.J. (2008) Enterococcus faecalis PcfC, a spatially localized substrate receptor for Type IV secretion of the pCF10 transfer intermediate. J. Bacteriol. 190 (10), 3632-3645.
25. Beranek, A., Zettl, M., Lorenzoni, K., Schauer, A., Manhart, M. and Koraimann, G. (2004) Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like Conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM. J. Bacteriol. 186 (20), 6999-7006. (Pubitemid 39332138)
26. Lu, L., Wong, J.J.W., Edwards, R.A., Manchak, J., Frost, L.S. and Glover, M.J.N. (2008) Structural basis of specific TraD-TraM recognition during F plasmidmediated bacterial conjugation. Mol. Microbiol. 70 (1), 89-99.
27. Szpirer, C., Faelen, M. and Couturier, M. (2000) Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob. Mol. Microbiol. 37 (6), 1283-1292.
28. Thomas, J. and Hecht, D.W. (2007) Interaction of Bacteroides fragilis pLV22a relaxase and transfer DNA with Escherichia coli RP4-TraG coupling protein. Mol. Microbiol. 66 (4), 948-960. (Pubitemid 350050421)
29. Karimova, G., Ullmann, A. and Ladant, D. (2002) A bacterial two-hybrid system based on Cyclic AMP signalling cascade in: A Molecular Cloning Manual (Golemis, E., Ed.), pp. 447-488, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Chapter 26.
30. Grant, S.G., Jessee, J., Bloom, F.R. and Hanahan, D. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylationrestriction mutants. Proc. Natl. Acad. Sci. USA 87, 4645-4649.
31. Studier, F.W. and Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
32. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
33. Wiseman, T., Williston, S., Brandts, J.F. and Lin, L.N. (1989) Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal. Biochem. 179, 131-137. (Pubitemid 19149635)
34. Cooper, A. (1998) Microcalorimetry of protein-protein interactions. Methods Mol. Biol. 88, 11-22.
35. Miller, J.H. (1992) A short course in bacterial genetics: A laboratory manual and handbook for Escherichia coli and related bacteria, Cold Spring Harbor Lab. Press, Plainview, NY.