Crystal structure of vinculin in complex with vinculin binding site 50 (VBS50), the integrin binding site 2 (IBS2) of talin

Protein Science

Volumn 21, Issue 4, 2012, Pages 583-588

  Yogesha, S D ^a   Rangarajan, Erumbi S ^a   Vonrhein, Clemens ^b   Bricogne, Gerard ^b   Izard, Tina ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b GLOBAL PHASING LTD   (United Kingdom)

Author keywords

Crystallography; Cytoskeleton; Focal adhesion; Protein protein interaction

Indexed keywords

INTEGRIN; TALIN; VINCULIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; HUMAN; HUMAN TISSUE; INTEGRIN BINDING SITE 2; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; VINCULIN BINDING SITE 50;

ACTINS; BINDING SITES; CALORIMETRY; FOCAL ADHESIONS; HUMANS; INTEGRINS; MODELS, MOLECULAR; MULTIPROTEIN COMPLEXES; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CELL SURFACE; TALIN; VINCULIN; X-RAY DIFFRACTION;

EID: 84858689928     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2041     Document Type: Article

References (32)

1. Critchley DR (2009) Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annual Rev Biophys 38:235-254.
2. Calderwood DA (2004) Integrin activation. J Cell Sci 117:657-666.
3. Calderwood DA, Zent R, Grant R, Rees DJ, Hynes RO, Ginsberg MH (1999) The Talin head domain binds to integrin β subunit cytoplasmic tails and regulates integrin activation. J Biol Chem 274:28071-28074.
4. Moes M, Rodius S, Coleman SJ, Monkley SJ, Goormaghtigh E, Tremuth L, Kox C, van der Holst PP, Critchley DR, Kieffer N (2007) The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin β subunits to the cytoskeleton. J Biol Chem 282:17280-17288. (Pubitemid 47093206)
5. Gingras AR, Ziegler WH, Bobkov AA, Joyce MG, Fasci D, Himmel M, Rothemund S, Ritter A, Grossmann JG, Patel B, Bate N, Goult BT, Emsley J, Barsukov IL, Roberts GC, Liddington RC, Ginsberg MH, Critchley DR (2009) Structural determinants of integrin binding to the talin rod. J Biol Chem 284:8866-8876.
6. Tanentzapf G, Brown NH (2006) An interaction between integrin and the talin FERM domain mediates integrin activation but not linkage to the cytoskeleton. Nat Cell Biol 8:601-606. (Pubitemid 43827353)
7. Borgon RA, Vonrhein C, Bricogne G, Bois PR, Izard T (2004) Crystal structure of human vinculin. Structure 12:1189-1197. (Pubitemid 38900761)
8. Rangarajan ES, Lee JH, Yogesha SD, Izard T (2010) A helix replacement mechanism directs metavinculin functions. PLoS ONE 5:e10679.
9. Izard T, Evans G, Borgon RA, Rush CL, Bricogne G, Bois PR (2004) Vinculin activation by talin through helical bundle conversion. Nature 427:171-175. (Pubitemid 38094959)
10. Izard T, Vonrhein C (2004) Structural basis for amplifying vinculin activation by talin. J Biol Chem 279: 27667-27678. (Pubitemid 38812608)
11. Yogesha SD, Sharff A, Bricogne G, Izard T (2011) Intermolecular versus intramolecular interactions of the vinculin binding site 33 of talin. Protein Sci 20:1471-1476.
12. Fillingham I, Gingras AR, Papagrigoriou E, Patel B, Emsley J, Critchley DR, Roberts GC, Barsukov IL (2005) A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head. Structure 13:65-74. (Pubitemid 40092475)
13. Gingras AR, Vogel KP, Steinhoff HJ, Ziegler WH, Patel B, Emsley J, Critchley DR, Roberts GC, Barsukov IL (2006) Structural and dynamic characterization of a vinculin binding site in the talin rod. Biochemistry 45: 1805-1817. (Pubitemid 43222124)
14. Gingras AR, Ziegler WH, Frank R, Roberts GC, Critchley DR, Emsley J (2005) Mapping and consensus sequence identification for multiple vinculin binding sites within the talin rod. J Biol Chem 280: 37217-37224. (Pubitemid 41587806)
15. Bois PR, Borgon RA, Vonrhein C, Izard T (2005) Structural dynamics of α-actinin-vinculin interactions. Mol Cell Biol 25:6112-6122.
16. Bois PR, O'Hara BP, Nietlispach D, Kirkpatrick J, Izard T (2006) The vinculin binding sites of talin and α-actinin are sufficient to activate vinculin. J Biol Chem 281:7228-7236. (Pubitemid 43847490)
17. Izard T, Tran Van Nhieu G, Bois PR (2006) Shigella applies molecular mimicry to subvert vinculin and invade host cells. J Cell Biol 175:465-475.
18. Park H, Valencia-Gallardo C, Sharff A, Tran Van Nhieu G, Izard T (2011) A novel Vinculin binding site of the IpaA invasin of Shigella. J Biol Chem 286: 23214-23224.
19. Tran Van Nhieu G, Izard T (2007) Vinculin binding in its closed conformation by a helix addition mechanism. Embo J 26:4588-4596. (Pubitemid 350036624)
20. Park H, Lee JH, Cossart P, Gouin E, Izard T (2011) The Rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. J Biol Chem 286: 35096-35103.
21. Chen VB, Arendall WB, III, Headd JJ, Keedy DA, Immormino RM, Kapral GJ, Murray LW, Richardson JS, Richardson DC (2010) MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr 66:12-21.
22. Cheung TY, Fairchild MJ, Zarivach R, Tanentzapf G, Van Petegem F (2009) Crystal structure of the talin integrin binding domain 2. J Mol Biol 387: 787-793.
23. Gingras AR, Bate N, Goult BT, Patel B, Kopp PM, Emsley J, Barsukov IL, Roberts GC, Critchley DR (2010) Central region of talin has a unique fold that binds vinculin and actin. J Biol Chem 285:29577-29587.
24. Lee SE, Kamm RD, Mofrad MR (2007) Force-induced activation of talin and its possible role in focal adhesion mechanotransduction. J Biomech 40:2096-2106. (Pubitemid 46843013)
25. del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP (2009) Stretching single talin rod molecules activates vinculin binding. Science 323:638-641.
26. Vonrhein C, Flensburg C, Keller P, Sharff A, Smart O, Paciorek W, Womack T, Bricogne G (2011) Data processing and analysis with the autoPROC toolbox. Acta Crystallogr D Biol Crystallogr 67:293-302.
27. Kabsch W (1993) Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J Appl Crystallogr 26:795-800.
28. Evans P (2006) Scaling and assessment of data quality. Acta Crystallogr D Biol Crystallogr 62:72-82.
29. Matthews BW (1968) Solvent content of protein crystals. J Mol Biol 33:491-497.
30. Vagin A, Teplyakov A (1997) MOLREP: an automated program for molecular replacement. J Appl Cryst 30: 1022-1025. (Pubitemid 127485985)
31. Bricogne G, Blanc E, Brandl M, Flensburg C, Keller P, Paciorek P, Roversi P, Sharff A, Smart OS, Vonrhein C, Womack TO (2011) BUSTER version 2.9. Cambridge, United Kingdom: Global Phasing Ltd.
32. Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132. (Pubitemid 41742764)