φ2GFP10, a high-intensity fluorophage, enables detection and rapid drug susceptibility testing of Mycobacterium tuberculosis directly from sputum samples

Journal of Clinical Microbiology

Volumn 50, Issue 4, 2012, Pages 1362-1369

  Jain, Paras ^a   Hartman, Travis E ^a   Eisenberg, Nell ^a   O'Donnell, Max R ^a   Kriakov, Jordan ^a   Govender, Karnishree ^b   Makume, Mantha ^b   Thaler, David S ^a   Hatfull, Graham F ^c   Sturm, A Willem ^b   Larsen, Michelle H ^a   Moodley, Preshnie ^b   Jacobs, William R ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF KWAZULU NATAL   (South Africa)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; GREEN FLUORESCENT PROTEIN; ISONIAZID; KANAMYCIN; OXOFLOXACIN; RIFAMPICIN; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIOPHAGE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIOENGINEERING; CONTROLLED STUDY; DIAGNOSTIC TEST; FLUORESCENCE ANALYSIS; GENE EXPRESSION; HIGH INTENSITY FLUOROPHAGE; INCUBATION TIME; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; SPUTUM EXAMINATION;

MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 84858612722     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.06192-11     Document Type: Article

References (46)

1. Balaban NQ, Merrin J, Chait R, Kowalik L, Leibler S. 2004. Bacterial persistence as a phenotypic switch. Science 305:1622-1625. (Pubitemid 39296357)
2. Banada PP, et al. 2010. Containment of bioaerosol infection risk by the Xpert MTB/RIF assay and its applicability to point-of-care settings. J. Clin. Microbiol. 48:3551-3557.
3. Banaiee N, et al. 2001. Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico. J. Clin. Microbiol. 39:3883-3888. (Pubitemid 33029755)
4. Banaiee N, et al. 2003. Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages. J. Med. Microbiol. 52:557-561. (Pubitemid 36871893)
5. Bardarov S, et al. 2002. Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis. Microbiology 148(Pt. 10):3007-3017. (Pubitemid 35243700)
6. Barker LP, Porcella SF, Wyatt RG, Small PL. 1999. The Mycobacterium marinum G13 promoter is a strong sigma 70-like promoter that is expressed in Escherichia coli and mycobacteria species. FEMS Microbiol. Lett. 175:79-85. (Pubitemid 29246056)
7. Blakemore R, et al. 2010. Evaluation of the analytical performance of the Xpert MTB/RIF assay. J. Clin. Microbiol. 48:2495-2501.
8. Boehme CC, et al. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005-1015.
9. Brown KL, Sarkis GJ, Wadsworth C, Hatfull GF. 1997. Transcriptional silencing by the mycobacteriophage L5 repressor.EMBOJ. 16:5914-5921. (Pubitemid 27427287)
10. Cattamanchi A, et al. 2008. Poor performance of universal sample processing method for diagnosis of pulmonary tuberculosis by smear microscopy and culture in Uganda. J. Clin. Microbiol. 46:3325-3329.
11. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. 1994. Green fluorescent protein as a marker for gene-expression. Science 263:802-805. (Pubitemid 24093206)
12. Cohen T, et al. 2010. The prevalence and drug sensitivity of tuberculosis among patients dying in hospital in KwaZulu-Natal, South Africa: a postmortem study. PLoS Med. 7:e1000296.
13. Colangeli R, et al. 2005. The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol. Mol. Microbiol. 55:1829-1840. (Pubitemid 40445216)
14. Davanloo P, Rosenberg AH, Dunn JJ, Studier FW. 1984. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U. S. A. 81:2035-2039. (Pubitemid 14146599)
15. David HL, Clavel S, Clement F. 1980. Adsorption and growth of the bacteriophage D29 in selected mycobacteria. Ann. Inst. Pasteur Virol. 131: 167-184. (Pubitemid 10073573)
16. Garton NJ, et al. 2008. Cytological and transcript analyses reveal fat and lazy persister-like bacilli in tuberculous sputum. PLoS Med. 5:e75.
17. Golomb M, Chamberlin M. 1974. Characterization of T7-specific ribonucleic acid polymerase. IV. Resolution of the major in vitro transcripts by gel electrophoresis. J. Biol. Chem. 249:2858-2863.
18. Gumbo T, et al. 2007. Concentration-dependent Mycobacterium tuberculosis killing and prevention of resistance by rifampin. Antimicrob. Agents Chemother. 51:3781-3788. (Pubitemid 350057771)
19. Gunther EJ, Murray NE, Glazer PM. 1993. High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E. coli lysogen. Nucleic Acids Res. 21:3903-3904. (Pubitemid 23254524)
20. Hassim S, et al. 2010. Detection of a substantial rate of multidrug-resistant tuberculosis in an HIV-infected population in South Africa by active monitoring of sputum samples. Clin. Infect. Dis. 50:1053-1059.
21. Helb D, et al. 2010. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48:229-237.
22. Hickey MJ, et al. 1996. Luciferase in vivo expression technology: use of recombinant mycobacterial reporter strains to evaluate antimycobacterial activity in mice. Antimicrob. Agents Chemother. 40:400-407. (Pubitemid 26053139)
23. Jacobs WR, Jr, et al. 1993. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science 260:819-822.
24. Jain P, et al. 2011. Reporter phage and breath tests: emerging phenotypic assays for diagnosing active tuberculosis, antibiotic resistance, and treatment efficacy. J. Infect. Dis. 204:S1142-S1150.
25. Keeler E, et al. 2006. Reducing the global burden of tuberculosis: the contribution of improved diagnostics. Nature 444(Suppl. 1):49-57.
26. Kent PT, Kubica GP. 1985. Isolation procedures, public health mycobacteriology: a guide for the level III laboratory. U.S. Dept. of Health and Human Services, Public Health Service, Centers for Disease Control, Atlanta, GA.
27. Maus CE, Plikaytis BB, Shinnick TM. 2005. Molecular analysis of crossresistance to capreomycin, kanamycin, amikacin, and viomycin in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 49:3192-3197. (Pubitemid 41060560)
28. Melzer M. 2011. An automated molecular test for Mycobacterium tuberculosis and resistance to rifampin (Xpert MTB/RIF) is sensitive and can be carried out in less than 2 h. Evid. Based Med. 16:19.
29. Moore DA, et al. 2004. Microscopic observation drug susceptibility assay, a rapid, reliable diagnostic test for multidrug-resistant tuberculosis suitable for use in resource-poor settings. J. Clin. Microbiol. 42:4432-4437. (Pubitemid 39362351)
30. Nagai T, et al. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20: 87-90. (Pubitemid 34044921)
31. Nesbit CE, Levin ME, Donnelly Wu MK, Hatfull GF. 1995. Transcriptional regulation of repressor synthesis in mycobacteriophage L5. Mol. Microbiol. 17:1045-1056.
32. Pearson RE, Jurgensen S, Sarkis GJ, Hatfull GF, Jacobs WR, Jr. 1996. Construction of D29 shuttle phasmids and luciferase reporter phages for detection of mycobacteria. Gene 183:129-136. (Pubitemid 27007501)
33. Piuri M, Jacobs WR, Jr, Hatfull GF. 2009. Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis. PLoS One 4:e4870.
34. Rastogi N, Goh KS, David HL. 1989. Drug susceptibility testing in tuberculosis: a comparison of the proportion methods using Lowenstein-Jensen, Middlebrook 7H10 and 7H11 agar media and a radiometric method. Res. Microbiol. 140:405-417. (Pubitemid 19252251)
35. Riska PF, et al. 1999. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. J. Clin. Microbiol. 37:1144-1149. (Pubitemid 29140627)
36. Rondón L, et al. 2011. Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. J. Clin. Microbiol. 49:1838-1842.
37. Rybniker J, et al. 2010. Insights into the function of the WhiB-like protein of mycobacteriophage TM4-a transcriptional inhibitor of WhiB2. Mol. Microbiol. 77:642-657.
38. Sambandamurthy VK, et al. 2006. Mycobacterium tuberculosis ΔRD1ΔpanCD: a safe and limited replicating mutant strain that protects immunocompetent and immunocompromised mice against experimental tuberculosis. Vaccine 24:6309-6320. (Pubitemid 44247236)
39. Sandgren A, et al. 2009. Tuberculosis drug resistance mutation database. PLoS Med. 6:e2.
40. Snapper SB, Melton RE, Mustafa S, Kieser T, Jacobs WR, Jr. 1990. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4:1911-1919.
41. Steingart KR, et al. 2006. Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review. Lancet Infect. Dis. 6:664-674. (Pubitemid 44462695)
42. Stover CK, et al. 1991. New use of BCG for recombinant vaccines. Nature 351:456-460. (Pubitemid 21896614)
43. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89. (Pubitemid 20219811)
44. Van Rie A, Page-Shipp L, Scott L, Sanne I, Stevens W. 2010. Xpert((R)) MTB/RIF for point-of-care diagnosis of TB in high-HIV burden, resource-limited countries: hype or hope? Expert Rev. Mol. Diagn. 10:937-946.
45. Wang F, et al. 2010. Mycobacterium tuberculosis dihydrofolate reductase is not a target relevant to the antitubercular activity of isoniazid. Antimicrob. Agents Chemother. 54:3776-3782.
46. WHO. 2011. Fluorescent light-emitting diode (LED) microscopy for diagnosis of tuberculosis. World Health Organization, Geneva, Switzerland. http://whqlibdoc.who.int/publications/2011/9789241501613-eng.pdf.