Discrimination of Trichophyton tonsurans and Trichophyton equinum by PCR-RFLP and by β-tubulin and Translation Elongation Factor 1-α sequencing

Medical Mycology

Volumn 50, Issue 7, 2012, Pages 760-764

  Rezaei Matehkolaei, Ali ^a,b   Makimura, Koichi ^c   De Hoog, G Sybren ^d,e   Shidfar, Mohammad Reza ^a   Satoh, Kazuo ^c   Najafzadeh, Mohammad Javad ^f   Mirhendi, Hossein ^a  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c TEIKYO UNIVERSITY   (Japan)

^d CBS KNAW FUNGAL BIODIVERSITY CENTRE   (Netherlands)

^e UNIVERSITY OF AMSTERDAM   (Netherlands)

^f MASHHAD UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

tubulin; ITS; PCR RFLP; Translation elongation factor 1 ; Trichophyton equinum; Trichophyton tonsurans

Indexed keywords

BETA TUBULIN; ELONGATION FACTOR 1ALPHA;

ARTICLE; BACTERIAL STRAIN; CONSENSUS SEQUENCE; DERMATOPHYTE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SINGLE NUCLEOTIDE POLYMORPHISM; TRICHOPHYTON MENTAGROPHYTES; TRICHOPHYTON TONSURANS;

ANIMALS; BASE SEQUENCE; DNA, FUNGAL; DNA, RIBOSOMAL SPACER; HUMANS; INDEL MUTATION; MOLECULAR SEQUENCE DATA; MYCOLOGY; PEPTIDE ELONGATION FACTOR 1; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; TRICHOPHYTON; TUBULIN;

ARTHRODERMATACEAE; EQUIDAE; TRICHOPHYTON EQUINUM; TRICHOPHYTON TONSURANS;

EID: 84858375532     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693786.2012.661885     Document Type: Article

References (15)

1. Brasch J, Logering B, Graser Y. Tinea capitis caused by Trichophyton equinum . Acta Derm Venereol 2009; 89: 204-205.
2. Woodgyer A. The curious adventures of T richophyton equinum in the realm of molecular biology: A modern fairy tale. Med Mycol 2004; 42: 397-403.
3. Ilkit M, Ali Saracli M, Kurdak H, et al . Clonal outbreak of T richophyton tonsurans tinea capitis gladiatorum among wrestlers in Adana, Turkey. Med Mycol 2010; 48: 480-485.
4. Jackson CJ, Barton RC, Evans EG. Species identifi cation and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol 1999; 37: 931-936.
5. Mochizuki T, Tanabe H, Kawasaki M, Ishizaki H, Jackson CJ. Rapid identifi cation of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions. J Dermatol Sci 2003; 32: 25-32.
6. Summerbell RC, Moore MK, Starink-Willemse M, Van Iperen A. ITS barcodes for Trichophyton tonsurans and T. equinum . Med Myco 2007; 45: 193-200.
7. Makimura K, Tamura Y, Mochizuki T, et al . Phylogenetic classifi cation and species identifi cation of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 1999; 37: 920-924.
8. White TJ, Bruns T, Taylor J. Amplifi cation and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.). PCR Protocols: A Guide to Methods and Applications . London: Academic Press, 1990: 315-322.
9. O ' Donnell K, Cigelnik E. Two different intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 1997; 7: 103-116.
10. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from fi lamentous ascomycetes. Appl Environ Microbiol 1995; 61: 1323-1330.
11. Rezaei-Matehkolaei A, Makimura K, De Hoog GS, et al . Multilocus differentiation of the relative dermatophytes Microsporum canis, M. ferrugineum and M. audouinii . J Med Microbiol 2012; 61: 57-63.
12. Graser Y, Kuijpers A, Presber W, De Hoog GS. Molecular taxonomy of Trichophyton mentagrophytes and T. tonsurans . Med Mycol 1999; 37: 315-330.
13. Summerbell RC, Haugland R, Li A, Gupta AK. Ribosomal RNA gene internal transcribed spacer 1 and 2 sequences of asexual, anthropophilic dermatophytes related to Trichophyton rubrum . J Clin Microbio 1999; 37: 4005-4011.
14. Graser Y, Scott J, Summerbell R. The new species concept in dermatophytes-a polyphasic approach. Mycopathologia 2008; 166: 239-256.
15. Abdel-Rahman SM, Sugita T, Gonz a lez GM, et al . Divergence among an international population of Trichophyton tonsurans isolates. Mycopathologia 2010; 169: 1-13.