Methods for growing and titrating african swine fever virus: Field and laboratory samples

Current Protocols in Cell Biology

Volumn , Issue SUPPL.53, 2011, Pages

  Carrascosa, Angel L ^a   Bustos, M Jose ^a   de Leon, Patricia ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

African swine fever virus (ASFV); COS 1; Cytopathic effect; Hemadsorption; IPAM; Plaque assay; Production; Purification; susceptibility; Swine macrophages; Target cells; Titration; VERO; WSL

Indexed keywords

AFRICAN SWINE FEVER VIRUS; ARTICLE; ERYTHROCYTE; GEL FILTRATION; LUNG ALVEOLUS MACROPHAGE; MONOCYTE; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; VERO CELL; VIROGENESIS; VIRUS ISOLATION; VIRUS TITRATION; AFRICAN SWINE FEVER; ANIMAL; CELL CULTURE; CELL LINE; CULTURE TECHNIQUE; CYTOPATHOGENIC EFFECT; DENSITY GRADIENT CENTRIFUGATION; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MACROPHAGE; METHODOLOGY; PHYSIOLOGY; SWINE; VIROLOGY; VIRUS CULTURE;

AFRICAN SWINE FEVER VIRUS; SUIDAE;

AFRICAN SWINE FEVER; AFRICAN SWINE FEVER VIRUS; ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CYTOPATHOGENIC EFFECT, VIRAL; MACROPHAGES; MONOCYTES; SWINE; VIRAL PLAQUE ASSAY; VIRUS CULTIVATION;

EID: 84858300396     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb2614s53     Document Type: Article

References (19)

1. Aguero, M., Fernandez, J., Romero, L., Sanchez Mascaraque, C., Arias, M., and Sanchez-Vizcaino, J.M. 2003. Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples. J. Clin. Microbiol. 41:4431-4434.
2. Barderas, M.G., Wigdorovitz, A., Merelo, F., Beitia, F., Alonso, C., Borca, M.V., and Escribano, J.M. 2000. Serodiagnosis of African swine fever using the recombinant protein p30 expressed in insect larvae. J. Virol. Methods. 89:129-136.
3. Bustos, M.J., Nogal, M.L., Revilla, Y., and Carrascosa, A.L. 2002. Plaque assay for African swine fever virus on swine macrophages. Arch. Virol. 147:1453-1459.
4. Carrascosa, A.L., Santaren, J.F., and Vinuela, E. 1982. Production and titration of African swine fever virus in porcine alveolar macrophages. J. Virol. Methods 3:303-310.
5. Carrascosa, A.L., del Val, M., Santaren, J.F., and Vinuela, E. 1985. Purification and properties of African swine fever virus. J. Virol. 54:337-344.
6. Casal, I., Enjuanes, L., and Vinuela, E. 1984. Porcine leukocyte cellular subsets sensitive to African swine fever virus in vitro. J. Virol. 52:37-46.
7. Dixon, L.K., Abrams, C.C., Bowick, G., Goatley, L.C., Kay-Jackson, P.C., Chapman, D., Liverani, E., Nix, R., Silk, R., and Zhang, F. 2004. African swine fever virus proteins involved in evading host defence systems. Vet. Immunol. Immunopathol. 100:117-134.
8. Enjuanes, L., Carrascosa, A.L., Moreno, M.A., and Vinuela, E. 1976. Titration of African swine fever (ASF) virus. J. Gen. Virol. 32:471-477.
9. Galindo, I., Almaźan, F., Bustos, M.J., Vi{ogonek}nuela, E., and Carrascosa, A.L. 2000. African Swine Fever Virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells. Virology 266:340-351.
10. Garcia, R., Almazan, F., Rodriguez, J.M., Alonso, M., Vinuela, E., and Rodriguez, J.F. 1995. Vectors for the genetic manipulation of African swine fever virus. J. Biotechnol. 40:121-131.
11. Granja, A.G., Nogal, M.L., Hurtado, C., Del Aguila, C., Carrascosa, A.L., Salas, M.L., Fresno, M., and Revilla, Y. 2006. The viral protein A238L inhibits TNF-{alpha} expression through a CBP/p300 transcriptional coactivators pathway. J. Immunol. 176:451-462.
12. Hurtado, C., Bustos, M.J., and Carrascosa, A.L. 2010. The use of COS-1 cells for studies of field and laboratory African swine fever virus samples. J. Virol. Methods 164:131-134.
13. Oura, C.A., Powell, P.P., and Parkhouse, R.M. 1998. Detection of African swine fever virus in infected pig tissues by immunocytochemistry and in sity hybridisation. J. Virol. Methods 72:205-217.
14. Parker, J. and Plowright, W. 1968. Plaque formation by African swine fever virus. Nature 219:524-525.
15. Pastor, M.J., Laviada, M.D., Sanchez-Vizcaino, J.M., and Escribano, J.M. 1989. Detection of African swine fever virus antibodies by immunoblotting assay. Can. J. Vet. Res. 53:105-107.
16. Pastor, M.J., Arias, M., Alcaraz, C., De Diego, M., and Escribano, J.M. 1992. A sensitive dot immunobinding assay for serodiagnosis of African swine fever virus with application in field conditions. J. Vet. Diagn. Invest. 4:254-257.
17. Vinuela, E. 1985. African swine fever virus. Curr. Top. Microbiol. Immunol. 116:151-170.
18. Wilkinson, P.J. 1989. African swine fever virus. In Virus Infections of Porcines (M.B. Pensaert, ed.) pp. 17-37. Elsevier Sciences Publishers, Amsterdam.
19. Zsak, L., Borca, M.V., Risatti, G.R., Zsak, A., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., and Rock, D.L. 2005. Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay. J. Clin. Microbiol. 43:112-119.