Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation

Bioresource Technology

Volumn 110, Issue , 2012, Pages 510-516

  Cheirsilp, Benjamas ^a   Torpee, Salwa ^a  

^a PRINCE OF SONGKLA UNIVERSITY   (Thailand)

Author keywords

Biodiesel; Chlorella sp.; Lipid; Microalgae; Nannochloropsis sp.

Indexed keywords

BATCH CULTIVATION; BIODIESEL PRODUCTION; CHLORELLA SP; CULTURE CONDITIONS; ENHANCED GROWTH; FATTY ACID COMPOSITION; FED-BATCH CULTIVATION; GLUCOSE CONCENTRATION; HETEROTROPHIC CULTURE; LIGHT INTENSITY; LIPID CONTENT; LIPID PRODUCTIONS; MICRO-ALGAE; MIXOTROPHIC; NANNOCHLOROPSIS SP;

ALGAE; BIODIESEL; GLUCOSE; LIPIDS; MICROORGANISMS;

ECOLOGY;

ARACHIDIC ACID; BIODIESEL; CHLOROPHYLL; DECANOIC ACID; GLUCOSE; HEPTADECANOIC ACID; LAURIC ACID; LINOLEIC ACID; LINOLENIC ACID; LIPID; MYRISTIC ACID; OLEIC ACID; PALMITIC ACID; PALMITOLEIC ACID; STEARIC ACID;

BIOFUEL; BIOMASS; GLUCOSE; GROWTH RATE; HETEROTROPHY; LIGHT INTENSITY; LIPID; MICROALGA; MIXOTROPHY; PHOTOAUTOTROPHY;

ALGAL CELL CULTURE; ALGAL GROWTH; ARTICLE; BIOFUEL PRODUCTION; BIOMASS PRODUCTION; CHEATOCEROS; CHLORELLA; CHLORELLA PROTOTHECOIDES; CHLORELLA SOROKINIANA; CHLORELLA VULGARIS; CONCENTRATION (PARAMETERS); CULTURE OPTIMIZATION; FED BATCH CULTURE; GROWTH INHIBITION; HETEROTROPHIC CULTURE; INTERMETHOD COMPARISON; LIGHT INTENSITY; LIPOGENESIS; MICROALGA; MIXOTROPHIC CULTURE; NANNOCHLOROPSIS; NONHUMAN; PHOTOAUTOTROPHIC CULTURE; PRIORITY JOURNAL;

BIOMASS; GLUCOSE; LIGHT; LIPIDS; MICROALGAE;

CHLORELLA SP.; NANNOCHLOROPSIS SP.;

EID: 84858276062     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2012.01.125     Document Type: Article

References (35)

1. Becker E.W. Microalgae: Biotechnology and Microbiology 1994, Cambridge University Press, New York.
2. Borowitzka M.A. Commercial production of microalgae: ponds, tanks, tubes and fermenters. J. Biotechnol. 1999, 70:313-321.
3. Cerón García M.C., Sánchez Mirón A., Fernández Sevilla J.M., Molina Grima E., García Camacho F. Mixotrophic growth of the microalga Phaeodactylum tricornutum influence of different nitrogen and organic carbon sources on productivity and biomass composition. Process Biochem. 2005, 40(1):297-305.
4. Chen F., Johns M.R. Substrate inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Process Biochem. 1994, 29:245-252.
5. Chojnacka K., Noworyta A. Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures. Enzyme Microb. Technol. 2004, 34:461-465.
6. Converti A., Casazza A.A., Ortiz E.Y., Perego P., Borghi M.D. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem. Eng. Process. 2009, 48(6):1146-1151.
7. Dumrattana P., Tansakul P. Effect of photoperiod on growth and hydrocarbon content of Botryococcus braunii cultured in effluent from seafood processing plant. Songklanakarin J. Sci. Technol. 2006, 28(1):99-105.
8. Doan T.T.Y., Sivaloganathan B., Obbard J.P. Screening of marine microalgae for biodiesel feedstock. Biomass Bioenergy 2011, 35(7):2534-2544.
9. Feng P., Deng Z., Hu Z., Fan L. Lipid accumulation and growth of Chlorella zofingiensis in flat plate photobioreactors outdoors. Bioresour. Technol. 2011, 102(22):10577-10584.
10. Folch J., Lees M., Stanley G.H.S. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957, 226:497-509.
11. Haass D., Tanner W. Regulation of hexose transport in Chlorella vulgaris. Plant Physiol. 1974, 53:291-302.
12. Heredia-Arroyo T., Wei W., Hu B. Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides. Appl. Biochem. Biotechnol. 2010, 162:1978-1995.
13. Hu H., Gao K. Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources. Biotechnol. Lett. 2003, 25:421-425.
14. Jeon Y.C.H., Cho C.H.W., Yun Y.S. Combined effects of light intensity and acetate concentration on the growth of unicellular microalga Haematococcus pluvialis. Enzyme Microb. Technol. 2006, 39:490-495.
15. Jham G.N., Teles F.F.F., Campos L.G. Use of aqueous HCl/MeOH as esterification reagent for analysis of fatty acids derived from soybean lipids. J. Am. Oil Chem. Soc. 1982, 59(3):132-133.
16. Lee Y.K. Algal nutrition. Heterotrophic carbon nutrition. Handbook of Microalgal Culture. Biotechnology and Applied Phycology 2004, 116. Blackwell Publishing, Oxford, UK. A. Richmond (Ed.).
17. Lewitus A.J., Kana T.M. Light respiration in six estuarine phytoplankton clones: contrasts under autotrophic and mixotrophic growth conditions. J. Phycol. 1995, 31:754-761.
18. Liang Y.N., Sarkany N., Cui Y. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol. Lett. 2009, 31:1043-1049.
19. Liu J., Huang J., Sun Z., Zhong Y., Jiang Y., Chen F. Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: assessment of algal oils for biodiesel production. Bioresour. Technol. 2011, 102(1):106-110.
20. Marquez F.J., Nishio N., Nagai S., Sasaki K. Enhancement of biomass and pigment production during growth of Spirulina platensis in mixotrophic culture. J. Chem. Technol. Biotechnol. 1995, 62:159-164.
21. Marquez F.J., Sasaki K., Kakizono T., Nishio N., Nagai S. Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. J. Ferment. Bioeng. 1993, 5:408-410.
22. Martínez M.E., Camacho F., Jiménez J.M., Espínola J.B. Influence of light intensity on the kinetic and yield parameters of Chlorella pyrenoidosa mixotrophic growth. Process Biochem. 1997, 32(3):93-98.
23. Metzger P., Largeau C. Chemicals of Botryococcus braunii. Chemicals from Microalgae 1999, 205-260. Taylor & Francis, London. Z. Cohen (Ed.).
24. Miao X., Wu Q. Biodiesel production from heterotrophic microalgal oil. Bioresour. Technol. 2006, 97:841-846.
25. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959, 31:426-429.
26. Minowa T., Yokoyama S.Y., Kishimoto M., Okakurat T. Oil production from algal cells of Dunaliella tertiolecta by direct thermochemical liquefaction. Fuel 1995, 74:1735-1738.
27. Ogbonna J.C., Tanaka H.C. Cyclic autotrophic/heterotrophic cultivation of photosynthetic cells: a method of achieving continuous cell growth under light/dark cycles. Bioresour. Technol. 1998, 65:62-72.
28. Sánchez S., Martínez M.E., Espejo M.T., Pacheco R., Espinola F., Hodaifa G. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. J. Appl. Phycol. 2001, 13:443-449.
29. Shi X.M., Zhang X.W., Chen F. Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources. Enzyme Microb. Technol. 2000, 27:312-318.
30. Tansakul P., Savaddiraksa Y., Prasertsan P., Tongurai C. Cultivation of the hydrocarbon-rich alga, Botyococcus braunii in secondary treated effluent from a sea food processing plant. Thai J. Agric. Sci. 2005, 38(1-2):71-76.
31. Villarejo A., Orús M.I., Martínez F. Coordination of photosynthetic and respiratory metabolism in Chlorella vulgaris UAM 101 in the light. Physiol. Plant. 1995, 94:680-686.
32. Xu H., Miao X., Wu Q. High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J. Biotechnol. 2006, 126:499-507.
33. 2 concentration change in Nannochloropsis sp. culture medium. Aquacult. Eng. 1995, 14(4):357-365.
34. Yeesang C., Cheirsilp B. Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresour. Technol. 2011, 102(3):3034-3040.
35. Zheng Y., Chi Z., Lucker B., Chen S. Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production. Bioresour. Technol. 2012, 103(1):484-488.