Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children

Journal of Clinical Virology

Volumn 53, Issue 4, 2012, Pages 308-313

  Hayden, Randall T ^a   Gu, Zhengming ^a   Rodriguez, Alicia ^a   Tanioka, Lisa ^c   Ying, Claire ^c   Morgenstern, Markus ^a   Bankowski, Matthew J ^b,c  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^b UNIVERSITY OF HAWAII   (United States)

^c Diagnostic Laboratory Services (The Queen's Medical Center)   (United States)

Author keywords

Immunocompromised; Multiplex real time PCR; Respiratory tract; Viral detection

Indexed keywords

ADENOVIRUS; ADOLESCENT; ADULT; ARTICLE; BOCAVIRUS; CHILD; CONTROLLED STUDY; CORONAVIRUS; ENTEROVIRUS; HUMAN; IMMUNOCOMPROMISED PATIENT; INFANT; INFLUENZA VIRUS A; INFLUENZA VIRUS B; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 3; PARAINFLUENZA VIRUS 4; PRESCHOOL CHILD; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY VIRUS; RHINOVIRUS; SCHOOL CHILD; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION;

ADOLESCENT; CHILD; CHILD, PRESCHOOL; HUMANS; IMMUNOCOMPROMISED HOST; INFANT; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; RESPIRATORY SYSTEM; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; VIRUS DISEASES; VIRUSES;

EID: 84858155877     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2011.12.020     Document Type: Article

References (36)

1. Bellau-Pujol S., Vabret A., Legrand L., Dina J., Gouarin S., Petitjean-Lecherbonnier J., et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 2005, 126(1/2):53-63.
2. Boivin G., Cote S., Dery P., De Serres G., Bergeron M.G. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J Clin Microbiol 2004, 42(1):45-51.
3. Bonroy C., Vankeerberghen A., Boel A., De Beenhouwer H. Use of a multiplex real-time PCR to study the incidence of human metapneumovirus and human respiratory syncytial virus infections during two winter seasons in a Belgian paediatric hospital. Clin Microbiol Infect 2007, 13(5):504-509.
4. Brittain-Long R., Nord S., Olofsson S., Westin J., Anderson L.M., Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 2008, 41(1):53-56.
5. Hindiyeh M., Hillyard D.R., Carroll K.C. Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens. Am J Clin Pathol 2001, 116(2):218-224.
6. Kehl S.C., Henrickson K.J., Hua W., Fan J. Evaluation of the Hexaplex assay for detection of respiratory viruses in children. J Clin Microbiol 2001, 39(5):1696-1701.
7. Templeton K.E., Scheltinga S.A., Beersma M.F., Kroes A.C., Claas E.C. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol 2004, 42(4):1564-1569.
8. Mahony J., Chong S., Merante F., Yaghoubian S., Sinha T., Lisle C., et al. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay. J Clin Microbiol 2007, 45(9):2965-2970.
9. Nolte F.S., Marshall D.J., Rasberry C., Schievelbein S., Banks G.G., Storch G.A., et al. MultiCode-PLx system for multiplexed detection of seventeen respiratory viruses. J Clin Microbiol 2007, 45(9):2779-2786.
10. Caliendo A.M. Multiplex PCR and emerging technologies for the detection of respiratory pathogens. Clin Infect Dis 2011, 52(Suppl. 4):S326-S330.
11. Mahony J.B. Detection of respiratory viruses by molecular methods. Clin Microbiol Rev 2008, 21(4):716-747.
12. Mahony J.B. Nucleic acid amplification-based diagnosis of respiratory virus infections. Expert Rev Anti Infect Ther 2010, 8(11):1273-1292.
13. Allander T., Tammi M.T., Eriksson M., Bjerkner A., Tiveljung-Lindell A., Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 2005, 102(36):12891-12896.
14. Falsey A.R., Erdman D., Anderson L.J., Walsh E.E. Human metapneumovirus infections in young and elderly adults. J Infect Dis 2003, 187(5):785-790.
15. Jartti T., van den Hoogen B., Garofalo R.P., Osterhaus A.D., Ruuskanen O. Metapneumovirus and acute wheezing in children. Lancet 2002, 360(9343):1393-1394.
16. Kahn J.S. Human metapneumovirus: a newly emerging respiratory pathogen. Curr Opin Infect Dis 2003, 16(3):255-258.
17. van den Hoogen B.G., de Jong J.C., Groen J., Kuiken T., de Groot R., Fouchier R.A., et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 2001, 7(6):719-724.
18. van der Hoek L., Pyrc K., Jebbink M.F., Vermeulen-Oost W., Berkhout R.J., Wolthers K.C., et al. Identification of a new human coronavirus. Nat Med 2004, 10(4):368-373.
19. Woo P.C., Lau S.K., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., et al. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol 2005, 79(2):884-895.
20. Lassauniere R., Kresfelder T., Venter M. A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses. J Virol Methods 2010, 165(2):254-260.
21. Balada-Llasat J.M., LaRue H., Kelly C., Rigali L., Pancholi P. Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults. J Clin Virol 2011, 50(1):42-45.
22. Schindera C., Kraemer A.L., Regamey N., Aebi C., Gorgievski-Hrisoho M., Barbani M.T. Immunofluorescence versus xTAG multiplex PCR for the detection of respiratory picornavirus infections in children. J Clin Virol 2010, 48(3):223-225.
23. Bezerra P.G., Britto M.C., Correia J.B., Duarte Mdo C., Fonceca A.M., Rose K., et al. Viral and atypical bacterial detection in acute respiratory infection in children under five years. PLoS One 2011, 6(4):e18928.
24. Arens M.Q., Buller R.S., Rankin A., Mason S., Whetsell A., Agapov E., et al. Comparison of the Eragen multi-code respiratory virus panel with conventional viral testing and real-time multiplex PCR assays for detection of respiratory viruses. J Clin Microbiol 2010, 48(7):2387-2395.
25. Sanghavi S.K., Bullotta A., Husain S., Rinaldo C.R. Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections. J Med Virol 2012, 84(1):162-169.
26. Guittet V., Brouard J., Vabret A., Lafay F., Guillois B., Duhamel J.F., et al. Rhinovirus and acute respiratory infections in hospitalized children retrospective study 1998-2000. Arch Pediatr 2003, 10(5):417-423.
27. Calvo C., Garcia-Garcia M.L., Blanco C., Vazquez M.C., Frias M.E., Perez-Brena P., et al. Multiple simultaneous viral infections in infants with acute respiratory tract infections in Spain. J Clin Virol 2008, 42(3):268-272.
28. Semple M.G., Cowell A., Dove W., Greensill J., McNamara P.S., Halfhide C., et al. Dual infection of infants by human metapneumovirus and human respiratory syncytial virus is strongly associated with severe bronchiolitis. J Infect Dis 2005, 191(3):382-386.
29. Rand K.H., Rampersaud H., Houck H.J. Comparison of two multiplex methods for detection of respiratory viruses: FilmArray RP and xTAG RVP. J Clin Microbiol 2011, 49(7):2449-2453.
30. Tapparel C., Junier T., Gerlach D., Cordey S., Van Belle S., Perrin L., et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 2007, 8:224.
31. Ali S.A., Gern J.E., Hartert T.V., Edwards K.M., Griffin M.R., Miller E.K., et al. Real-world comparison of two molecular methods for detection of respiratory viruses. Virol J 2011, 8:332.
32. Lieberman D., Shimoni A., Keren-Naus A., Steinberg R., Shemer-Avni Y. Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling. J Clin Microbiol 2009, 47(11):3439-3443.
33. Lieberman D., Shimoni A., Keren-Naus A., Steinberg R., Shemer-Avni Y. Pooled nasopharyngeal and oropharyngeal samples for the identification of respiratory viruses in adults. Eur J Clin Microbiol Infect Dis 2010, 29(6):733-735.
34. Smieja M., Castriciano S., Carruthers S., So G., Chong S., Luinstra K., et al. Development and evaluation of a flocked nasal midturbinate swab for self-collection in respiratory virus infection diagnostic testing. J Clin Microbiol 2010, 48(9):3340-3342.
35. She R.C., Polage C.R., Caram L.B., Taggart E.W., Hymas W.C., Woods C.W., et al. Performance of diagnostic tests to detect respiratory viruses in older adults. Diagn Microbiol Infect Dis 2010, 67(3):246-250.
36. Mahony J.B., Blackhouse G., Babwah J., Smieja M., Buracond S., Chong S., et al. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol 2009, 47(9):2812-2817.