Detection of PIGO-deficient cells using proaerolysin: A valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  Nakamura, Jun ^a   Gul, Husamettin ^a,b   Tian, Xu ^a   Bultman, Scott J ^c   Swenberg, James A ^a,d  

^a University of North Carolina at Chapel Hill   (United States)

^b GULHANE SCHOOL OF MEDICINE   (Turkey)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOTOXIN; GLYCOSYLPHOSPHATIDYLINOSITOL; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; MESYLIC ACID METHYL ESTER; PROAEROLYSIN; UNCLASSIFIED DRUG; BACTERIAL TOXIN; DIAGNOSTIC AGENT; PORE FORMING CYTOTOXIC PROTEIN;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL ASSAY; CELL STRAIN; CELL STRAIN DT40; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOTOXICITY; FRAMESHIFT MUTATION; GENE DELETION; GENE IDENTIFICATION; GENE INSERTION; GENE LOCATION; GENE SEQUENCE; MARKER GENE; MUTAGENESIS; MUTAGENICITY; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; NUCLEOTIDE SEQUENCE; PHENOTYPE; PIG O GENE; SEX CHROMOSOME; ANALYSIS OF VARIANCE; ANIMAL; AT RICH SEQUENCE; BIOSYNTHESIS; CELL LINE; CELL SURVIVAL; CHICKEN; DNA REPAIR; FLOW CYTOMETRY; METHODOLOGY; MUTAGEN TESTING; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

SUIDAE;

ANALYSIS OF VARIANCE; ANIMALS; AT RICH SEQUENCE; BACTERIAL TOXINS; CELL LINE; CELL SURVIVAL; CHICKENS; DNA REPAIR; FLOW CYTOMETRY; GLYCOSYLPHOSPHATIDYLINOSITOLS; METHYL METHANESULFONATE; MUTAGENESIS; MUTAGENICITY TESTS; PORE FORMING CYTOTOXIC PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84858134469     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0033563     Document Type: Article

References (39)

1. Baba TW, Giroir BP, Humphries EH, (1985) Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151.
2. Sonoda E, Morrison C, Yamashita YM, Takata M, Takeda S, (2001) Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40. Philos Trans R Soc Lond B Biol Sci 356: 111-117.
3. Dhar PK, Sonoda E, Fujimori A, Yamashita YM, Takeda S, (2001) DNA repair studies: Experimental evidence in support of chicken DT40 cell line as a unique model. J Environ Pathol Toxicol Oncol 20: 273-283.
4. Winding P, Berchtold MW, (2001) The chicken B cell line DT40: A novel tool for gene disruption experiments. J Immunol Methods 249: 1-16.
5. Yamazoe M, Sonoda E, Hochegger H, Takeda S, (2004) Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40. DNA Repair (Amst) 3: 1175-1185.
6. Ridpath JR, Nakamura A, Tano K, Luke AM, Sonoda E, et al. (2007) Cells deficient in the FANC/BRCA pathway are hypersensitive to plasma levels of formaldehyde. Cancer Res 67: 11117-11122.
7. Ridpath JR, Takeda S, Swenberg JA, Nakamura J, (2010) Convenient, multi-well plate-based DNA damage response analysis using DT40 mutants is applicable to a High-throughput genotoxicity assay with characterization of modes of action. Environ Mol Mutagen 52: 153-160.
8. Strauss GH, Albertini RJ, (1979) Enumeration of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo. Mutat Res 61: 353-379.
9. Aidoo A, Morris SM, Casciano DA, (1997) Development and utilization of the rat lymphocyte hprt mutation assay. Mutat Res 387: 69-88.
10. Von Tungeln LS, Williams LD, Doerge DR, Shaddock JG, McGarrity LJ, et al. (2007) Transplacental drug transfer and frequency of Tk and Hprt lymphocyte mutants and peripheral blood micronuclei in mice treated transplacentally with zidovudine and lamivudine. Environ Mol Mutagen 48: 258-269.
11. Miura D, Dobrovolsky VN, Kasahara Y, Katsuura Y, Heflich RH, (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat. Environ Mol Mutagen 49: 614-621.
12. Bryce SM, Bemis JC, Dertinger SD, (2008) In vivo mutation assay based on the endogenous Pig-a locus. Environ Mol Mutagen 49: 256-264.
13. Kawagoe K, Takeda J, Endo Y, Kinoshita T, (1994) Molecular cloning of murine pig-a, a gene for GPI-anchor biosynthesis, and demonstration of interspecies conservation of its structure, function, and genetic locus. Genomics 23: 566-574.
14. Watanabe R, Inoue N, Westfall B, Taron CH, Orlean P, et al. (1998) The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1. EMBO J 17: 877-885.
15. Nishimura J, Murakami Y, Kinoshita T, (1999) Paroxysmal nocturnal hemoglobinuria: An acquired genetic disease. Am J Hematol 62: 175-182.
16. Brodsky RA, Hu R, (2006) PIG-A mutations in paroxysmal nocturnal hemoglobinuria and in normal hematopoiesis. Leuk Lymphoma 47: 1215-1221.
17. Kinoshita T, Fujita M, Maeda Y, (2008) Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress. J Biochem 144: 287-294.
18. Low MG, (1989) The glycosyl-phosphatidylinositol anchor of membrane proteins. Biochim Biophys Acta 988: 427-454.
19. Cross GA, (1990) Glycolipid anchoring of plasma membrane proteins. Annu Rev Cell Biol 6: 1-39.
20. Miura D, Dobrovolsky VN, Mittelstaedt RA, Kasahara Y, Katsuura Y, et al. (2008) Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants. Environ Mol Mutagen 49: 622-630.
21. Parker MW, van der Goot FG, Buckley JT, (1996) Aerolysin-the ins and outs of a model channel-forming toxin. Mol Microbiol 19: 205-212.
22. Diep DB, Nelson KL, Raja SM, Pleshak EN, Buckley JT, (1998) Glycosylphosphatidylinositol anchors of membrane glycoproteins are binding determinants for the channel-forming toxin aerolysin. J Biol Chem 273: 2355-2360.
23. MacKenzie CR, Hirama T, Buckley JT, (1999) Analysis of receptor binding by the channel-forming toxin aerolysin using surface plasmon resonance. J Biol Chem 274: 22604-22609.
24. Abrami L, Fivaz M, Glauser PE, Parton RG, van der Goot FG, (1998) A pore-forming toxin interacts with a GPI-anchored protein and causes vacuolation of the endoplasmic reticulum. J Cell Biol 140: 525-540.
25. Maeda Y, Ashida H, Kinoshita T, (2006) CHO glycosylation mutants: GPI anchor. Methods Enzymol 416: 182-205.
26. Hong Y, Maeda Y, Watanabe R, Inoue N, Ohishi K, et al. (2000) Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol. J Biol Chem 275: 20911-20919.
27. Jacobs L, Bean CL, Marx JA, (1983) Optimal phenotypic expression times for HPRT mutants induced in foreskin-, skin-, and lung-derived human diploid fibroblasts. Environ Mutagen 5: 717-731.
28. Doak SH, Jenkins GJ, Johnson GE, Quick E, Parry EM, et al. (2007) Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens. Cancer Res 67: 3904-3911.
29. de Latour RP, Mary JY, Salanoubat C, Terriou L, Etienne G, et al. (2008) Paroxysmal nocturnal hemoglobinuria: natural history of disease subcategories. Blood 112: 3099-3106.
30. Tiede A, Bastisch I, Schubert J, Orlean P, Schmidt RE, (1999) Biosynthesis of glycosylphosphatidylinositols in mammals and unicellular microbes. Biol Chem 380: 503-523.
31. Klungland A, Laake K, Hoff E, Seeberg E, (1995) Spectrum of mutations induced by methyl and ethyl methanesulfonate at the hprt locus of normal and tag expressing Chinese hamster fibroblasts. Carcinogenesis 16: 1281-1285.
32. Calléja F, Jansen JG, Vrieling H, Laval F, van Zeeland AA, (1999) Modulation of the toxic and mutagenic effects induced by methyl methanesulfonate in Chinese hamster ovary cells by overexpression of the rat N-alkylpurine-DNA glycosylase. Mutat Res 425: 185-194.
33. Elder RH, Jansen JG, Weeks RJ, Willington MA, Deans B, et al. (1998) Alkylpurine-DNA-N-glycosylase knockout mice show increased susceptibility to induction of mutations by methyl methanesulfonate. Mol Cell Biol 18: 5828-5837.
34. Nivard MJ, Pastink A, Vogel EW, (1992) Molecular analysis of mutations induced in the vermilion gene of Drosophila melanogaster by methyl methanesulfonate. Genetics 131: 673-682.
35. Nivard MJ, Pastink A, Vogel EW, (1993) Impact of DNA nucleotide excision repair on methyl methanesulfonate-induced mutations in Drosophila melanogaster. Carcinogenesis 14: 1585-1590.
36. Simonelli V, Narciso L, Dogliotti E, Fortini P, (2005) Base excision repair intermediates are mutagenic in mammalian cells. Nucleic Acids Res Aug 2;33 (14): 4404-11.
37. Russo D, Fronza G, Ottaggio L, Monti P, Inga A, et al. (2009) High frequency of genomic deletions induced by Me-lex, a sequence selective N3-adenine methylating agent, at the Hprt locus in Chinese hamster ovary cells. Mutat Res 671: 58-66.
38. Hong Y, Ohishi K, Inoue N, Kang JY, Shime H, et al. (2002) Requirement of N-glycan on GPI-anchored proteins for efficient binding of aerolysin but not Clostridium septicum alpha-toxin. EMBO J 21: 5047-5056.
39. Furth EE, Thilly WG, Penman BW, Liber HL, Rand WM, (1981) Quantitative assay for mutation in diploid human lymphoblasts using microtiter plates. Anal Biochem 110: 1-8.