In vitro model of invasive pulmonary aspergillosis in the human alveolus

Methods in Molecular Biology

Volumn 845, Issue , 2012, Pages 361-367

  Gregson, Lea ^a   Hope, William W ^a   Howard, Susan J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Aspergillosis; Aspergillus; Tissue culture

Indexed keywords

ARTICLE; ASPERGILLUS FUMIGATUS; BIOLOGICAL MODEL; CULTURE TECHNIQUE; ENDOTHELIUM CELL; EPITHELIUM CELL; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; LUNG ALVEOLUS; LUNG ASPERGILLOSIS; METHODOLOGY; MICROBIOLOGY; PATHOLOGY;

ASPERGILLUS FUMIGATUS; CELL CULTURE TECHNIQUES; ENDOTHELIAL CELLS; EPITHELIAL CELLS; HUMANS; INVASIVE PULMONARY ASPERGILLOSIS; MODELS, BIOLOGICAL; PULMONARY ALVEOLI;

ASPERGILLUS;

EID: 84858122222     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-539-8_24     Document Type: Chapter

References (6)

1. Birkness, K. A., Deslauriers, M., Bartlett, J. H., White, E. H., King, C. H., and Quinn, F. D. (1999) An in vitro tissue culture bilayer model to examine early events in Mycobacterium tuberculosis infection. Infect Immun 67, 653–8.
2. Bermudez, L. E., Sangari, F. J., Kolonoski, P., Petrofsky, M., and Goodman, J. (2002) The efficiency of the translocation of Mycobacterium tuberculosis across a bilayer of epithelial and endothelial cells as a model of the alveolar wall is a consequence of transport within mononuclear phagocytes and invasion of alveolar epithelial cells. Infect Immun 70, 140–6.
3. Hope, W. W., Kruhlak, M. J., Lyman, C. A., Petraitiene, R., Petraitis, V., Francesconi, A., Kasai, M., Mickiene, D., Sein, T., Peter, J., Kelaher, A. M., Hughes, J. E., Cotton, M. P., Cotten, C. J., Bacher, J., Tripathi, S., Bermudez, L., Maugel, T. K., Zerfas, P. M., Wingard, J. R., Drusano, G. L., and Walsh, T. J. (2007) Pathogenesis of Aspergillus fumigatus and the kinetics of galactomannan in an in vitro model of early invasive pulmonary aspergillosis: implications for antifungal therapy. J Infect Dis 195, 455–66.
4. Lestner, J. M., Howard, S. J., Goodwin, J., Gregson, L., Majithiya, J., Walsh, T. J., Jensen, G. M., and Hope, W. W. (2010). Pharmacokinetics and pharmacodynamics of amphotericin B deoxycholate, liposomal amphotericin B, and amphotericin B lipid complex in an in vitro model of invasive pulmonary aspergillosis. Antimicrob Agents Chemother 54, 3432–41.
5. Wasylnka, J. A., and Moore, M. M. (2002) Uptake of Aspergillus fumigatus conidia by phagocytic and nonphagocytic cells in vitro: quantitation using strains expressing green fluorescent protein. Infect Immun 70, 3156–63.
6. Wasylnka, J. A., and Moore, M. M. (2003) Aspergillus fumigatus conidia survive and germinate in acidic organelles of A549 epithelial cells. J Cell Sci 116, 1579–87.