PolyA RT-PCR-based quantification of microRNA by using universal TaqMan probe

Biotechnology Letters

Volumn 34, Issue 4, 2012, Pages 627-633

  Luo, Xin ^a   Zhang, Jin ^a   Wang, Huijun ^a   Du, Yingying ^a   Yang, Lu ^a   Zheng, Fengyun ^a   Ma, Duan ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

microRNA; PolyA RT PCR; SYBR Green; TaqMan probe

Indexed keywords

CELL LINES; HIGH-THROUGHPUT; MICRORNAS; MOUSE TISSUES; PATHOLOGICAL CONDITIONS; POLYA RT-PCR; PROBE-BASED; QUANTIFICATION METHODS; SYBR GREEN; TAQMAN PROBES;

CELL CULTURE; HISTOLOGY; POLYMERASE CHAIN REACTION; TISSUE;

PROBES;

MICRORNA; POLYADENYLIC ACID;

ANIMAL; ARTICLE; EVALUATION; GENETICS; METHODOLOGY; MOLECULAR BIOLOGY; MOUSE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION;

ANIMALS; MICE; MICRORNAS; MOLECULAR BIOLOGY; OLIGONUCLEOTIDE PROBES; POLY A; POLYMERASE CHAIN REACTION;

EID: 84858008308     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0813-3     Document Type: Article

References (14)

1. Barad O, Meiri E, Avniel A, Aharonov R, Barzilai A et al (2004) MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues. Genome Res 14(12): 2486-2494.
2. Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endorinol 25(2): 169-193.
3. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33(20): e179.
4. Chen Y, Gelfond JA, McManus LM, Shireman PK (2009) Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis. BMC Genomics 10: 407.
5. Chen C, Tan R, Wong L, Fekete R, Halsey J (2011) Quantitation of microRNAs by real-time RT-qPCR. Methods Mol Biol 687: 113-134.
6. Ivey KN, Muth A, Arnold J, King FW et al (2008) MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. Cell Stem Cell 2(3): 219-229.
7. Jiang J, Lee EJ, Gusev Y, Schmittgen TD (2005) Real-time expression profiling of microRNA precursors in human cancer cell lines. Nucleic Acids Res 33(17): 5394-5403.
8. Mattie MD, Benz CC, Bowers J, Sensinger K, Wong L, Scott GK, Fedele V, Ginzinger D, Getts R, Haqq C (2006) Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies. Mol Cancer 5: 24.
9. Nelson PT, Baldwin DA, Scearce LM, Oberholtzer JC, Tobias JW, Mourelatos Z (2004) Microarray-based, high-throughput gene expression profiling of microRNAs. Nat Methods 1(2): 155-161.
10. Raymond CK, Roberts BS, Garrett-Engele P, Lim LP, Johnson JM (2005) Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 11(11): 1737-1744.
11. Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP (2002) MicroRNAs in plants. Genes Dev 16(13): 1616-1626.
12. Schaefer A, Jung M, Miller K, Lein M, Kristiansen G, Erbersdobler A, Jung K (2010) Suitable reference genes for relative quantification of miRNA expression in prostate cancer. Exp Mol Med 42(11): 749-758.
13. Shi R, Chiang VL (2005) Facile means for quantifying microRNA expression by real-time PCR. BioTechniques 39(4): 519-525.
14. Zhang J, Du YY, Lin YF, Chen YT, Yang L, Wang HJ, Ma D (2008) The cell growth suppressor, mir-126, targets IRS-1. Biochem Biophys Res Commun 377(1): 136-140.