Dark pixel intensity determination and its applications in normalizing different exposure time and autofluorescence removal

Journal of Microscopy

Volumn 246, Issue 1, 2012, Pages 1-10

  Pang, Z ^a   Laplante, N E ^a   Filkins, R J ^a  

^a GENERAL ELECTRIC COMPANY   (United States)

Author keywords

Autofluorescence removal; Dark current; Dark pixel intensity; Exposure time; Readout noise; Standardization

Indexed keywords

CAMERAS; IMAGE ACQUISITION; PIXELS;

AUTOFLUORESCENCE; AUTOFLUORESCENCE REMOVAL; DARK PIXEL INTENSITY; EXPOSURE-TIME; GRAY-SCALE IMAGES; IMAGES PROCESSING; ITS APPLICATIONS; LINEAR RANGE; PIXEL INTENSITIES; READOUT NOISE;

STANDARDIZATION;

ARTICLE; AUTOFLUORESCENCE; CALCULATION; CAMERA; DIGITAL IMAGING; ELECTRON; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; IMAGE SUBTRACTION; LIGHT EXPOSURE; LIGHT INTERCEPTION; OPTICAL RESOLUTION; PIXEL INTENSITY; PRIORITY JOURNAL; SIGNAL NOISE RATIO;

EID: 84857995786     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2011.03581.x     Document Type: Article

References (20)

1. Aubin, J.E. (1979) Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27, 36-43.
2. Barash, E., Fomitchov, P.A., Filkins, R.J.M., Mayers M., Sood, A., Potyrailo, R.A., Xia, H. & Montalto, M. (2008) Absorption and emission red shift in stained cell and tissue samples. Am. Biotechnol. Lab. 26, 12-14.
3. Benson, R.C., Meyer, R.A., Zaruba, M.E. & McKhann, G.M. (1979) Cellular autofluorescence-is it due to flavins? J. Histochem. Cytochem. 27, 44-48.
4. Bissonnette, R., Zeng, H., McLean, D.I., Schreiber, W.E., Roscoe, D.L. & Lui, H. (1998) Psoriatic plaques exhibit red autofluorescence that is due to protoporphyrin IX. J. Invest. Dermatol. 111, 586-591.
5. Camp, R.L., Chung, G.G. & Rimm, D.L. (2002) Automated subcellular localization and quantification of protein expression in tissue microarrays. Nat. Med. 8, 1323-1327.
6. Chen, Q.-S., Defrise, M. & Deconinck, F. (1994) Symmetric phase-only matched filtering of Fourier-Mellin transforms for image registration and recognition. IEEE Trans. Pattern Anal. Mach. Intell. 16, 1156-1168.
7. Chung, G.G., Zerkowski, M.P., Ghosh, S., Camp, R.L. & Rimm, D.L. (2007) Quantitative analysis of estrogen receptor heterogeneity in breast cancer. Lab. Invest. 87, 662-669.
8. Deyl, Z., Macek, K., Adam, M. & Vancikova, O. (1980) Studies on the chemical nature of elastin fluorescence. Biochim. Biophys. Acta. 625, 248-254.
9. Fitzmaurice, M., Bordagaray, J.O., Engelmann, G.L., Richards-Kortum, R., Kolubayev, T., Feld, M.S., Ratliff, N.B. & Kramer, J.R. (1989) Argon ion laser-excited autofluorescence in normal and atherosclerotic aorta and coronary arteries: morphologic studies. Am. Heart. J. 118, 1028-1038.
10. Linkert, M., Rueden, C.T., Allan, C., et al. (2010) Metadata matters: access to image data in the real world. J. Cell Biol. 189, 777-782.
11. Mansfield, J.R., Gossage, K.W., Hoyt, C.C. & Levenson, R.M. (2005) Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging. J. Biomed. Opt. 10, 41207.
12. McCabe, A., Dolled-Filhart, M., Camp, R.L. & Rimm, D.L. (2005) Automated quantitative analysis (AQUA) of in situ protein expression, antibody concentration, and prognosis. J. Natl. Cancer Inst. 97, 1808-1815.
13. Pawley, J. (2000) The 39 steps: a cautionary tale of quantitative 3-D fluorescence microscopy. Biotechniques 28, 884-886, 888.
14. Rasband, W.S. (19972011) ImageJ. National Institutes of Health, Bethesda, Maryland, USA.
15. Sady, C., Khosrof, S. & Nagaraj, R. (1995) Advanced Maillard reaction and crosslinking of corneal collagen in diabetes. Biochem. Biophys. Res. Commun. 214, 793-797.
16. Schnell, S.A., Staines, W.A. & Wessendorf, M.W. (1999) Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J. Histochem. Cytochem. 47, 719-730.
17. Thevenaz, P., Ruttimann, U.E. & Unser, M. (1998) A pyramid approach to subpixel registration based on intensity. IEEE Trans. Image Process., 7, 27-41.
18. Van de Lest, C.H., Versteeg, E.M., Veerkamp, J.H. & Van Kuppevelt, T.H. (1995) Elimination of autofluorescence in immunofluorescence microscopy with digital image processing. J. Histochem. Cytochem. 43, 727-730.
19. Wilson, J. & Hawkes, J. (1998) Optoelectronics an Introduction. 3rd edn. Prentice Hall PTR, London.
20. Woolfe, F., Gerdes, M., Bello, M., Tao, X. & Can, A. (2011) Autofluorescence removal by non-negative matrix factorization. IEEE Trans. Image Process 20, 1085-1093.