Identification of consistent alkylation of cysteine-less peptides in a proteomics experiment

Biochemical and Biophysical Research Communications

Volumn 419, Issue 2, 2012, Pages 305-308

  Woods, Alisa G ^a   Sokolowska, Izabela ^a   Darie, Costel C ^a  

^a CLARKSON UNIVERSITY   (United States)

Author keywords

Database search; Iodoacetamide; Mass spectrometry; Peptide alkylation; Proteomics

Indexed keywords

CYSTEINE; PEPTIDE;

ALKYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEOMICS;

ALKYLATING AGENTS; ALKYLATION; AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; CYSTEINE; DATABASES, PROTEIN; IODOACETAMIDE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 84857995363     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.02.016     Document Type: Article

References (9)

1. Aebersold R., Mann M. Mass spectrometry-based proteomics. Nature 2003, 422:198-207.
2. Boja E.S., Fales H.M. Overalkylation of a protein digest with iodoacetamide. Anal. Chem. 2001, 73:3576-3582.
3. Darie C.C., Biniossek M.L., Jovine L., Litscher E.S., Wassarman P.M. Structural characterization of fish egg vitelline envelope proteins by mass spectrometry. Biochemistry 2004, 43:7459-7478.
4. Shevchenko A., Wilm M., Vorm O., Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 1996, 68:850-858.
5. Lapko V.N., Smith D.L., Smith J.B. Identification of an artifact in the mass spectrometry of proteins derivatized with iodoacetamide. J. Mass Spectrom. 2000, 35:572-575.
6. Lundell N., Schreitmuller T. Sample preparation for peptide mapping - A pharmaceutical quality-control perspective. Anal. Biochem. 1999, 266:31-47.
7. Windsor W.T., Syto R., Tsarbopoulos A., Zhang R., Durkin J., Baldwin S., Paliwal S., Mui P.W., Pramanik B., Trotta P.P., et al. Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10. Biochemistry 1993, 32:8807-8815.
8. Yang Z., Attygalle A.B. LC/MS characterization of undesired products formed during iodoacetamide derivatization of sulfhydryl groups of peptides. J. Mass Spectrom. 2007, 42:233-243.
9. Sokolowska I., Woods A.G., Gawinowicz M.A., Roy U., Darie C.C. Identification of potential tumor differentiation factor (TDF) receptor from steroid-responsive and steroid-resistant breast cancer cells. J. Biol. Chem. 2012, 287:1719-1733.