Systematic use of universal 16S rRNA gene polymerase chain reaction (PCR) and sequencing for processing pleural effusions improves conventional culture techniques

Medicine

Volumn 91, Issue 2, 2012, Pages 103-110

  Insa, Rosario ^a   Marín, Mercedes ^a,b,c   Martín, Adoración ^a   Martín Rabadán, Pablo ^a,b,c   Alcalá, Luís ^a,b,c   Cercenado, Emilia ^a,b,c   Calatayud, Laura ^b,d   Liñares, Josefina ^b,c,d   Bouza, Emilio ^a,b,c  

^a HOSPITAL GENERAL UNIVERSITARIO GREGORIO MARAÑÓN   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c Red Española de Investigación en Patología Infecciosa REIPI   (Spain)

^d HOSPITAL UNIVERSITARI DE BELLVITGE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ADOLESCENT; ADULT; AGED; ARTICLE; CHILD; CONTROLLED STUDY; CULTURE TECHNIQUE; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FALSE POSITIVE RESULT; FEMALE; GENE SEQUENCE; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MICROBIOLOGICAL EXAMINATION; MOLECULAR DIAGNOSIS; MYCOBACTERIOSIS; PLEURA EFFUSION; PLEURA FLUID; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRESCHOOL CHILD; PRIORITY JOURNAL; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIA; BACTERIAL INFECTIONS; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; FEMALE; HUMANS; MALE; MIDDLE AGED; PLEURAL EFFUSION; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; YOUNG ADULT;

EID: 84857881653     PISSN: 00257974     EISSN: 15365964     Source Type: Journal    
DOI: 10.1097/MD.0b013e31824dfdb0     Document Type: Article

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