On-chip isotachophoresis for separation of ions and purification of nucleic acids

Journal of Visualized Experiments

Volumn , Issue 61, 2012, Pages

  Garcia Schwarz, Giancarlo ^a   Rogacs, Anita ^a   Bahga, Supreet S ^a   Santiago, Juan G ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

Bioengineering; Electrokinetics; Isotachophoresis; Issue 61; Microfluidics; Sample preparation

Indexed keywords

EID: 84857869705     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3890     Document Type: Review

References (18)

1. Jung, B., Bharadwaj, R., & Santiago, J.G. On-chip millionfold sample stacking using transient isotachophoresis. Anal. Chem. 78 (7), 2319 (2006).
2. Jung, B., Zhu, Y., & Santiago, J.G. Detection of 100 am fluorophores using a high-sensitivity on-chip CE system and transient isotachophoresis. Anal. Chem. 79 (1), 345 (2007).
3. Everaerts, F.M., Beckers, J.L., & Verheggen, T.P.E.M. Isotachophoresis: Theory, instrumentation, and applications. Elsevier Science & Technology., (1976).
4. Khurana, T.K. & Santiago, J.G. Preconcentration, separation, and indirect detection of nonfluorescent analytes using fluorescent mobility markers. Anal. Chem. 80 (1), 279 (2008).
5. Bercovici, M., Kaigala, G.V., Backhouse, C.J., & Santiago, J.G. Fluorescent carrier ampholytes assay for portable, label-free detection of chemical toxins in tap water. Anal. Chem. 82 (5), 1858 (2010).
6. Bercovici, M., Kaigala, G.V., & Santiago, J.G. Method for analyte identification using isotachophoresis and a fluorescent carrier ampholyte assay. Anal. Chem. 82 (5), 2134 (2010).
7. Kaigala, G.V., et al. Miniaturized system for isotachophoresis assays. Lab Chip. 10 (17), 2242 (2010).
8. Chambers, R.D. & Santiago, J.G. Imaging and quantification of isotachophoresis zones using nonfocusing fluorescent tracers. Anal. Chem. 81 (8), 3022 (2009).
9. Schoch, R.B., Ronaghi, M., & Santiago, J.G. Rapid and selective extraction, isolation, preconcentration, and quantitation of small RNAs from cell lysate using on-chip isotachophoresis. Lab Chip. 9 (15), 2145 (2009).
10. Persat, A., Marshall, L.A., & Santiago, J.G. Purification of nucleic acids from whole blood using isotachophoresis. Anal. Chem. 81 (22), 9507 (2009).
11. Bercovici, M., et al. Rapid detection of urinary tract infections using isotachophoresis and molecular beacons. Anal. Chem. 83 (11), 4110 (2011).
12. Marshall, L.A. & Santiago, J.G. Extraction of DNA from malaria-infected erythrocytes using isotachophoresis. Anal. Chem. 83 (24), 9715 (2011).
13. Persat, A., Chambers, R.D., & Santiago, J.G. Basic principles of electrolyte chemistry for microfluidic electrokinetics. Part I: Acid-base equilibria and pH buffers. Lab Chip. 9 (17), 2437 (2009).
14. Garcia-Schwarz, G., Bercovici, M., Marshall, L.A., & Santiago, J.G. Sample dispersion in isotachophoresis. J. Fluid Mech. 1 (1), 1 (2011).
15. Kohlrausch, F. Über concentrations-verschiebungen durch electrolyse im inneren von lösungen und lösungsgemischen. Ann. Phys. 298 (10), 209 (1897).
16. Alberty, R.A. Moving boundary systems formed by weak electrolytes. Theory of simple systems formed by weak acids and bases. J. Am. Chem. Soc. 72 (6), 2361 (1950).
17. Jovin, T.M. Multiphasic zone electrophoresis. I. Steady-state moving-boundary systems formed by different electrolyte combinations. Biochem. 12 (5), 871 (1973).
18. Persat, A. & Santiago, J.G. MicroRNA profiling by simultaneous selective isotachophoresis and hybridization with molecular beacons. Anal. Chem. (2011).