Detection of mycobacterium tuberculosis in sputum by gas chromatography-mass spectrometry of methyl mycocerosates released by thermochemolysis

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  O'Sullivan, Denise M ^a,e   Nicoara, Simona C ^b   Mutetwa, Reggie ^c   Mungofa, Stanley ^c   Lee, Oona Y C ^d   Minnikin, David E ^d   Bardwell, Max W ^b   Corbett, Elizabeth L ^a,c   McNerney, Ruth ^a   Morgan, Geraint H ^b  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b OPEN UNIVERSITY   (United Kingdom)

^c BIOMEDICAL RESEARCH AND TRAINING INSTITUTE   (Zimbabwe)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^e LGC   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; MYCOCEROSIC ACID METHYL ESTER; PETROLEUM ETHER; PHTHIOCEROL DIMYCOCEROSATE; TETRAMETHYLAMMONIUM HYDROXIDE; UNCLASSIFIED DRUG; FATTY ACID; LIPID; MYCOCEROSIC ACID;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; HUMAN; INTERMETHOD COMPARISON; LIPID ANALYSIS; LUNG TUBERCULOSIS; MASS FRAGMENTOGRAPHY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; SENSITIVITY AND SPECIFICITY; SINGLE BLIND PROCEDURE; SPUTUM ANALYSIS; SPUTUM SMEAR; THERMOCHEMOLYTIC MASS FRAGMENTOGRAPHY; TRANSESTERIFICATION; CULTURE TECHNIQUE; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; SPUTUM; STANDARD; TEMPERATURE;

MYCOBACTERIUM TUBERCULOSIS;

CULTURE TECHNIQUES; FATTY ACIDS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; LIPIDS; MYCOBACTERIUM TUBERCULOSIS; REFERENCE STANDARDS; SPUTUM; TEMPERATURE;

EID: 84857768309     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0032836     Document Type: Article

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