Convenient and rapid removal of detergent from glycolipids in detergent-resistant membrane microdomains

Journal of Lipid Research

Volumn 53, Issue 3, 2012, Pages 599-608

  Suzuki, Yusuke ^a,b   Kabayama, Kazuya ^a  

^a TOKAI UNIVERSITY   (Japan)

^b NIHON UNIVERSITY   (Japan)

Author keywords

Detergent removal; Ganglioside; Glycosphingolipid; MALDI mass spectrometry

Indexed keywords

1,2 DICHLOROETHANE; ACETONE; ACETONITRILE; AMPHOLYTE; BUTANOL; CHLOROFORM; CYCLOHEXANE; DETERGENT; DICHLOROETHANE; DICHLOROMETHANE; DIISOPROPYL ETHER; ETHER; GANGLIOSIDE; GANGLIOSIDE GM3; GLYCOLIPID; GLYCOSPHINGOLIPID; HEPTANE; HEXANE; METHANOL; ORGANIC SOLVENT; PYRIDINE; SIALIC ACID; TETRAHYDROFURAN; TOLUENE; TRITON X 100; XYLENE;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CELL MEMBRANE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY; HYDROGEN BOND; LIMIT OF DETECTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROADIPOCYTE; SUCROSE GRADIENT;

3T3-L1 CELLS; ANIMALS; CHROMATOGRAPHY, THIN LAYER; DETERGENTS; GANGLIOSIDES; GLYCOLIPIDS; MEMBRANE MICRODOMAINS; MICE; SOLVENTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 84857704580     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D020545     Document Type: Article

References (23)

1. Kolter, T., R. L. Proia, and K. Sandhoff. 2002. Combinatorial ganglioside biosynthesis. J. Biol. Chem. 277: 25859-25862. (Pubitemid 34967063)
2. Hakomori, S. 2003. Structure, organization, and function of glycosphingolipids in membrane. Curr. Opin. Hematol. 10: 16-24. (Pubitemid 36020713)
3. Iwabuchi, K., H. Nakayama, C. Iwahara, and K. Takamori. 2010. Significance of glycosphingolipid fatty acid chain length on membrane microdomain-mediated signal transduction. FEBS Lett. 584: 1642-1652.
4. Sonnino, S., A. Prinetti, L. Mauri, V. Chigorno, and G. Tettamanti. 2006. Dynamic and structural properties of sphingolipids as driving forces for the formation of membrane domains. Chem. Rev. 106: 2111-2125.
5. Kabayama, K., T. Sato, K. Saito, N. Loberto, A. Prinetti, S. Sonnino, M. Kinjo, Y. Igarashi, and J. Inokuchi. 2007. Dissociation of the insulin receptor and caveolin-1 complex by ganglioside GM3 in the state of insulin resistance. Proc. Natl. Acad. Sci. USA. 104: 13678-13683. (Pubitemid 350003380)
6. Kabayama, K., T. Sato, F. Kitamura, S. Uemura, B. W. Kang, Y. Igarashi, and J. Inokuchi. 2005. TNFalpha-induced insulin resistance in adipocytes as a membrane microdomain disorder: involvement of ganglioside GM3. Glycobiology. 15: 21-29. (Pubitemid 40202036)
7. Ohmi, Y., O. Tajima, Y. Ohkawa, Y. Yamauchi, Y. Sugiura, and K. Furukawa. 2011. Gangliosides are essential in the protection of inflammation and neurodegeneration via maintenance of lipid rafts: elucidation by a series of ganglioside-deficient mutant mice. J. Neurochem. 116: 926-935.
8. Thompson, T. E., and T. W. Tillack. 1985. Organization of glycosphingolipids in bilayers and plasma membranes of mammalian cells. Annu. Rev. Biophys. Biophys. Chem. 14: 361-386.
9. Arreaza, G., K. A. Melkonian, M. LaFevre-Bernt, and D. A. Brown. 1994. Triton X-100-resistant membrane complexes from cultured kidney epithelial cells contain the Src family protein tyrosine kinase p62yes. J. Biol. Chem. 269: 19123-19127.
10. Hakomori, S. 1998. Cancer-associated glycosphingolipid antigens: their structure, organization, and function. Acta Anat. (Basel). 161: 79-90. (Pubitemid 28478161)
11. Loo, R. R., N. Dales, and P. C. Andrews. 1996. The effect of detergents on proteins analyzed by electrospray ionization. Methods Mol. Biol. 61: 141-160.
12. Rundlett, K. L., and D. W. Armstrong. 1996. Mechanism of signal suppression by anionic surfactants in capillary electrophoresis-electrospray ionization mass spectrometry. Anal. Chem. 68: 3493-3497.
13. Zhang, N., and L. Li. 2004. Effects of common surfactants on protein digestion and matrix-assisted laser desorption/ionization mass spectrometric analysis of the digested peptides using two-layer sample preparation. Rapid Commun. Mass Spectrom. 18: 889-896. (Pubitemid 38520254)
14. Yeung, Y. G., and E. R. Stanley. 2010. Rapid detergent removal from peptide samples with ethyl acetate for mass spectrometry analysis. Curr. Protoc. Protein Sci. Chapter 16: Unit 16.12.
15. Rey, M., H. Mrazek, P. Pompach, P. Novak, L. Pelosi, G. Brandolin, E. Forest, V. Havlicek, and P. Man. 2010. Effective removal of nonionic detergents in protein mass spectrometry, hydrogen/deuterium exchange, and proteomics. Anal. Chem. 82: 5107-5116.
16. Yeung, Y. G., E. Nieves, R. H. Angeletti, and E. R. Stanley. 2008. Removal of detergents from protein digests for mass spectrometry analysis. Anal. Biochem. 382: 135-137.
17. Antharavally, B. S., K. A. Mallia, M. M. Rosenblatt, A. M. Salunkhe, J. C. Rogers, P. Haney, and N. Haghdoost. 2011. Efficient removal of detergents from proteins and peptides in a spin column format. Anal. Biochem. 416: 39-44.
18. Rubin, C. S., E. Lai, and O. M. Rosen. 1977. Acquisition of increased hormone sensitivity during in vitro adipocyte development. J. Biol. Chem. 252: 3554-3557. (Pubitemid 8113952)
19. Suzuki, Y., M. Yanagisawa, T. Ariga, and R. K. Yu. 2011. Histone acetylation-mediated glycosyltransferase gene regulation in mouse brain during development. J. Neurochem. 116: 874-880.
20. Shi, C., F. Han, Y. Xiong, and C. Tian. 2009. A gas chromatographymass spectrometry method to monitor detergents removal from a membrane protein sample. Protein Expr. Purif. 68: 221-225.
21. Balagopalan, L., E. Sherman, V. A. Barr, and L. E. Samelson. 2011. Imaging techniques for assaying lymphocyte activation in action. Nat. Rev. Immunol. 11: 21-33.
22. Lidke, D. S., and B. S. Wilson. 2009. Caught in the act: quantifying protein behaviour in living cells. Trends Cell Biol. 19: 566-574.
23. Tanaka, K. A., K. G. Suzuki, Y. M. Shirai, S. T. Shibutani, M. S. Miyahara, H. Tsuboi, M. Yahara, A. Yoshimura, S. Mayor, T. K. Fujiwara, et al. 2010. Membrane molecules mobile even after chemical fixation. Nat. Methods. 7: 865-866.