Stem-loop RT-qPCR for miRNAs

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 95, 2011, Pages

  Kramer, Martha F ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

MIQE; MiRNA; RT qPCR

Indexed keywords

COMPLEMENTARY DNA; MICRORNA; NUCLEIC ACID; NUCLEOTIDE;

ACCURACY; ARTICLE; BINDING AFFINITY; BINDING SITE; CLINICAL PROTOCOL; DNA SYNTHESIS; GENE STRUCTURE; GENE TARGETING; GENETIC ANALYSIS; HYDROLYSIS; LIMIT OF QUANTITATION; NOMENCLATURE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA BINDING; RNA PROBE; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; STANDARDIZATION; STRATEGIC PLANNING; TEMPERATURE SENSITIVITY; DILUTION; MELTING POINT; MOLECULAR PROBE; PROCESS DEVELOPMENT; REACTION ANALYSIS; RNA ANALYSIS; RNA SEQUENCE; RNA TRANSCRIPTION;

EID: 84857656168     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb1510s95     Document Type: Article

References (9)

1. Burroughs, A.M., Ando, Y., de Hoon, M.J.4., Tomaru, Y., Nishibu, T., Ukekawa, R., Funakoshi, T., Kurokawa, T., Suzuki, H., Hayashizaki1, Y., and Daubl, C.O. 2010. A comprehensive survey of 3' animal miRNA modification events and a possible role for 3' adenylation in modulating miRNA targeting effectiveness. Genome Res. 20:1398-1410.
2. Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M.W., Shipley, G.L., Vandesompele, J., and Wittwer, C.T. 2009. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55:1-12.
3. Chen, C., Ridzon, D.A., Broomer, A.J., Zhou, Z., Lee, D.H., Nguyen, J.T., Barbisin, M., Xu, N.L., Mahuvakar, V.R., Andersen, M.R., Lao, K.Q., Livak, K.J., and Guegler, K.J. 2005. Real-time quantification of microRNAs by stem-loop RTPCR. Nucleic Acids Res. 33:e179.
4. Cui, C.,Griffiths, A., Li, G., Silva, L.,Kramer, M.F., Gaasterland, T., Wang, X.J., and Coen, D.M. 2006. Prediction and identification of herpes simplex virus 1-encoded microRNAs. J. Virol. 80:5499-5508.
5. Cullen, B. 2004. Transcription and processing of human microRNA precursors. Mol. Cell 16:861-865.
6. Ibrahim, F., Rymarquis, L.A., Kim, E.J., Becker, J., Balassa, E., Green, P.J., and Cerutti, H. 2010. Uridylation of mature miRNAs andpromotes their degradation in Chlamydomonas. Proc. Natl. Acad. Sci. U.S.A. 107:3906-3911.
7. Jurak, I., Kramer, M.F.,Mellor, J.C., van Lint, A.L., Roth, F.P., Knipe, D.M., and Coen, D.M. 2010. Numerous conserved and divergent microRNAs expressed by herpes simplex viruses 1 and 2. J. Virol. 84:4659-4672.
8. Logan, J., Edwards, K., and Saunders, N. 2009. Real-Time PCR: Current Technology and Applications. Caister Academic Press, Norfolk, UK.
9. Umbach, J.L., Kramer, M.F., Jurak, I., Karnowski, H.W., Coen, D.M., and Cullen, B.R. 2008. MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs. Nature 454:780-783.