Properties of an 'LNA'-modified ricin RNA aptamer

Biochemical and Biophysical Research Communications

Volumn 419, Issue 1, 2012, Pages 60-65

  Förster, Charlotte ^a,c   Zydek, Martin ^b   Rothkegel, Maika ^a   Wu, Zhiyang ^a   Gallin, Claudia ^a   Geßner, Reinhard ^c   Lisdat, Fred ^b   Fürste, Jens P ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b TECHNICAL UNIVERSITY OF APPLIED SCIENCES WILDAU   (Germany)

^c Klinik und Poliklinik für Visceral Transplantations Thorax und Gefäßchirurgie   (Germany)

Author keywords

Binding affinity; LNA modification; Nuclease sensitivity; Ricin RNA aptamer; Thermostability

Indexed keywords

2' FLUORO RNA; 2' O 4'C METHYLENE BETA DEXTRO RIBOFURANOSE NUCLEIC ACID; 2' O METHYL RNA; APTAMER; DOUBLE STRANDED RNA; LOCKED NUCLEIC ACID; RIBONUCLEASE; RIBONUCLEASE A; RIBONUCLEASE T1; RIBONUCLEASE V1; RICIN; RNA APTAMER; SINGLE STRANDED RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN RNA BINDING; RNA STABILITY; TEMPERATURE; THERMOSTABILITY;

APTAMERS, NUCLEOTIDE; BASE SEQUENCE; GUANOSINE; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; RICIN; SELEX APTAMER TECHNIQUE;

EID: 84857655812     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.01.127     Document Type: Article

References (22)

1. Ellington A.D., Szostak J.W. In vitro selection of RNA molecules that bind specific ligands. Nature 1990, 346:818-822.
2. Tuerk C., Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249:505-510.
3. Hermann T., Patel D.J. Adaptive recognition by nucleic acid aptamers. Science 2000, 287:820-825.
4. Nimjee S.M., Rusconi C.P., Sullenger B.A. Aptamers: an emerging class of therapeutics. Annu. Rev. Med. 2005, 56:555-583.
5. Gryziewicz L. Regulatory aspects of drug approval for macular degeneration. Adv. Drug Deliv. Rev. 2005, 57:2092-2098.
6. Petersen M., Wengel J. LNA: a versatile tool for therapeutics and genomics trends. Biotechnology 2003, 21:74-81.
7. Petersen M., Bondensgaard K., Wengel J., Jacobsen J.P. Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids. J. Am. Chem. Soc. 2002, 124:5974-5982.
8. Vester B., Wengel J. LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA. Biochemistry 2004, 43:13233-13241.
9. Nielsen K.E., Rasmussen J., Kumar R., Wengel J., Jacobsen J.P., Petersen M. NMR studies of fully modified locked nucleic acid (LNA) hybrids: solution structure of an LNA:RNA hybrid and characterization of an LNA:DNA hybrid. Bioconj. Chem. 2004, 15:449-457.
10. Petersen M., Nielsen C.B., Nielsen K.E., Jensen G.A., Bondensgaard K., Singh S.K., Rajwanshi V.K., Koshkin A.A., Dahl B.M., Wengel J., Jacobsen J.P. The conformations of locked nucleic acids (LNA). J. Mol. Recognit. 2000, 13:44-53.
11. Hesselberth J.R., Miller D., Robertus J., Ellington A.D. In vitro selection of RNA molecules that inhibit the activity of ricin A-chain. J. Biol. Chem. 2000, 275:4937-4942.
12. Föster C., Oberthüer D., Gao J., Eichert A., Quast F.G., Betzel C., Nitsche A., Erdmann V.A., Füste J.P. Crystallization and preliminary X-ray diffraction data of an LNA 7-mer duplex derived from a ricin aptamer. Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 2009, 65:881-885.
13. Schmidt K.S., Borkowski S., Kurreck J., Stephens A.W., Bald R., Hecht M., Friebe M., Dinkelborg L., Erdmann V.A. Application of locked nucleic acids to improve aptamer in vivo stability and targeting function. Nucleic Acids Res. 2004, 32:5757-5765.
14. Tombelli S., Minunni M., Mascini M. Biosensors for RNA aptamers-protein interaction. Methods Mol. Biol. 2008, 419:109-119.
15. de-los-Santos-álvarez N., Lobo-Castanón M., Miranda-Ordieres A., Tunón-Blanco P. Aptamers as recognition elements for label-free analytical devices. Trends Anal. Chem. 2008, 437-446.
16. Lisdat F., Utepbergenov D., Haseloff R.F., Blasig I.E., Stocklein W., Scheller F.W., Brigelius-Flohe R. An optical method for the detection of oxidative stress using protein-RNA interaction. Anal. Chem. 2001, 73:957-962.
17. Eichert A., Behling K., Betzel C., Erdmann V.A., Fürste J.P., Förster C. The crystal structure of an 'All Locked' nucleic acid duplex. Nucleic Acids Res. 2010, 38:6729-6736.
18. Grune M., Fürste J.P., Klussmann S., Erdmann V.A., Brown L.R. Detection of multiple conformations of the E-domain of 5S rRNA from Escherichia coli in solution and in crystals by NMR spectroscopy. Nucleic Acids Res. 1996, 24:2592-2596.
19. Schlegel M.K., Essen L.O., Meggers E. Duplex structure of a minimal nucleic acid. J. Am. Chem. Soc. 2008, 130:8158-8159.
20. He W., Hatcher E., Balaeff A., Beratan D.N., Gil R.R., Madrid M., Achim C. Solution structure of a peptide nucleic acid duplex from NMR data: features and limitations. J. Am. Chem. Soc. 2008, 130:13264-13273.
21. Rasmussen H., Kastrup J.S., Nielsen J.N., Nielsen J.M., Nielsen P.E. Crystal structure of a peptide nucleic acid (PNA) duplex at 1.7A resolution. Nat. Struct. Biol. 1997, 4:98-101.
22. Egli M., Pallan P.S., Pattanayek R., Wilds C.J., Lubini P., Minasov G., Dobler M., Leumann C.J., Eschenmoser A. Crystal structure of homo-DNA and nature's choice of pentose over hexose in the genetic system. J. Am. Chem. Soc. 2006, 128:10847-10856.