Label-free spatial analysis of free and enzyme-bound NAD(P)H in the presence of high concentrations of melanin

Journal of Fluorescence

Volumn 22, Issue 1, 2012, Pages 349-355

  Herbrich, Sebastian ^a   Gehder, Matthias ^a   Krull, Rainer ^a   Gericke, Karl Heinz ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Fluorescence lifetime imaging; Forward convolution; Melanin; NAD(P)H; Spore

Indexed keywords

ASPERGILLUS OCHRACEUS; AUTOFLUORESCENCE; BIOLOGICAL SAMPLES; EMISSION FILTERS; EXCITATION WAVELENGTH; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE LIFETIMES; FUNGAL SPORES; HIGH CONCENTRATION; INSTRUMENT RESPONSE FUNCTIONS; LABEL FREE; MELANIN; MODEL ORGANISMS; MULTI-EXPONENTIAL DECAY; NAD(P)H; SPATIAL ANALYSIS; SPATIAL DIFFERENTIATION; SPORE; SUB-CELLULAR; TWO-PHOTON EXCITATION MICROSCOPIES;

CONVOLUTION; FLUORESCENCE;

FLUOROPHORES;

ENZYME; MELANIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; ASPERGILLUS OCHRACEUS; CHEMISTRY; CYTOLOGY; METABOLISM; METHODOLOGY; SPECTROFLUOROMETRY;

ASPERGILLUS OCHRACEUS; ENZYMES; MELANINS; NADP; SPECTROMETRY, FLUORESCENCE;

ASPERGILLUS OCHRACEUS; BACTERIA (MICROORGANISMS); FUNGI;

EID: 84857641837     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-011-0965-5     Document Type: Article

References (24)

1. Lakowicz JR (2006) Principles of fluorescence spectroscopy. Vol, 3rd edn. Springer, New York
2. Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59(1):89-113 (Pubitemid 351813025)
3. Imanishi Y, Lodowski KH, Koutalos Y (2007) Two-photon microscopy: shedding light on the chemistry of vision†. Biochemistry 46(34):9674-9684 (Pubitemid 47328573)
4. Meredith P, Sarna T (2006) The physical and chemical properties of eumelanin. Pigment Cell Res 19(6):572-594 (Pubitemid 44674110)
5. Durrell LW (1964) The composition and structure of walls of dark fungus spores. Mycopathologia 23(4):339-345
6. Bell AA, Wheeler MH (1986) Biosynthesis and functions of fungal melanins. Annu Rev Phytopathol 24(1):411-451
7. Quentmeier S, Quentmeier CC, Walla PJ, Gericke K-H (2009) Two-color two-photon excitation of intrinsic protein fluorescence: label-free observation of proteolytic digestion of bovine serum albumin. Chemphyschem 10(9-10):1607-1613
8. Tran ML, Powell BJ, Meredith P (2006) Chemical and structural disorder in eumelanins: a possible explanation for broadband absorbance. Biophys J 90(3):743-752
9. Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML (1992) Fluorescence lifetime imaging of free and protein-bound NADH. Proc Natl Acad Sci USA 89(4):1271-1275
10. Niesner R, Narang P, Spiecker H, Andresen V, Gericke K-H, Gunzer M (2008) selective detection of NADPH oxidase in polymorphonuclear cells by means of NAD(P)H-based fluorescence lifetime imaging. J Biophys, 2008
11. Niesner R, Bülent P, Schlüsche P, Gericke K-H (2004) Noniterative biexponential fluorescence lifetime imaging in the investigation of cellular metabolism by means of NAD(P)H autofluorescence. Chemphyschem 5(8):1141-1149
12. Elson D et al (2004) Time-domain fluorescence lifetime imaging applied to biological tissue. Photochem Photobiol Sci 3(8):795-801 (Pubitemid 39302981)
13. Gerritsen HC, Asselbergs MAH, Agronskaia AV, Van Sark WGJHM (2002) Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution. J Microsc 206(3):218-224 (Pubitemid 34639419)
14. Niesner R, Gericke K-H (2008) Fluorescence lifetime imaging in biosciences: technologies and applications. Front Phys 3(1)
15. Denicke S, Ehlers J-E, Niesner R, Quentmeier S, Gericke K-H (2007) Steady-state and time-resolved two-photon fluorescence microscopy: a versatile tool for probing cellular environment and function. Phys Scripta 76(3):C115
16. Carriles R, Schafer DN, Sheetz KE, Field JJ, Cisek R, Barzda V, Sylvester AW, Squier JA (2009) Invited review article: imaging techniques for harmonic and multiphoton absorption fluorescence microscopy. Rev Sci Instrum 80(8):081101
17. Zipfel WR, Williams RM, Webb WW (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21 (11):1369-1377 (Pubitemid 37356624)
18. Ehlers A, Riemann I, Stark M, König K (2007) Multiphoton fluorescence lifetime imaging of human hair. Microsc Res Tech 70 (2):154-161 (Pubitemid 46292250)
19. Habenicht A, Hjelm J, Mukhtar E, Bergström F, Johansson LBÅ (2002) Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments. Chem Phys Lett 354(5-6):367-375
20. Zheltikov AM (2006) Let there be white light: supercontinuum generation by ultrashort laser pulses. Phys Usp 49(6):605
21. Available from: http://www.picoquant.com/datasheets/photon-counting/PDM- Series.pdf.
22. Forest SE, Lam WC, Millar DP, Nofsinger JB, Simon JD (2000) A model for the activated energy transfer within eumelanin aggregates. J Phys Chem B 104(4):811-814
23. Zhang Q, Piston DW, Goodman RH (2002) Regulation of corepressor function by nuclear NADH. Science 295(5561):1895-1897 (Pubitemid 34214127)
24. Arcangeli C, Yu W, Cannistraro S, Gratton E (2000) Two-photon autofluorescence microscopy and spectroscopy of Antarctic fungus: new approach for studying effects of UV-B irradiation. Biopolymers 57(4):218-225 (Pubitemid 30415069)