Efficient expression of the anti-AahI' scorpion toxin nanobody under a new functional form in a Pichia pastoris system

Biotechnology and Applied Biochemistry

Volumn 59, Issue 1, 2012, Pages 15-21

  Ezzine, Aymen ^a   M'Hirsi El Adab, Sonia ^a   Bouhaouala Zahar, Balkiss ^b   Hmila, Issam ^b   Baciou, Laura ^c   Marzouki, Mohamed Najib ^a  

^a UNIVERSITY OF CARTHAGE   (Tunisia)

^b PASTEUR INSTITUTE OF TUNIS   (Tunisia)

^c UNIVERSITÉ PARIS SUD   (France)

Author keywords

AahI' toxin; heterologous protein; His Tag; N glycosylation; nanobody; Pichia pastoris

Indexed keywords

AAHI' TOXIN; HETEROLOGOUS PROTEINS; HIS-TAG; N-GLYCOSYLATION; NANOBODY; PICHIA PASTORIS;

AMINO ACIDS; ANTIBODIES; CELL CULTURE; CLONING; COVALENT BONDS; ESCHERICHIA COLI; PHYSIOLOGY; TOXIC MATERIALS;

YEAST;

ANTIBODY; COMPLEMENTARY DNA; NBAAHI22 ANTIBODY; NITRILOTRIACETATE NICKEL; SCORPION VENOM; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; GENE EXPRESSION; PICHIA; RECOMBINANT PROTEINS; SCORPIONS;

EID: 84857282320     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.67     Document Type: Article

References (38)

1. Eckart, M. R., and, Bussineau, C. M., (1996) Curr. Opin. Biotech. 7, 525-530.
2. Sleep, D., Belfield, G. P., Ballance, D. J., Steven, J., Jones, S., Evans, L. R., Moir, P. D., and, Goodey, A. R., (1991) Nat. Biotechnol. 9, 183-187.
3. Higgins, D. R., and, Cregg, J. M., (1998) Introduction to Pichia pastoris. In Pichia Protocols. (, D. R. Higginsand, and, J. M. Cregg, eds.). pp. 1-15, Humana Press, Totowa, New Jersey.
4. Hollenberg, C. P., and, Gellissen, G., (1997) Curr. Opin. Biotechnol. 8, 554-560.
5. Romanos, M. A., Scorer, C. A., and, Clare, J. J., (1992) 8, 423-488.
6. Cregg, J. M., (1999) Expression in the methylotrophic yeast Pichia pastoris. In Gene expression systems: Using nature for the art of expression (, Fernandez, J., and, Hoeffler, J., eds.) pp. 158-184, Academic Press, San Diego, California.
7. Cregg, J. M., Madden, K. R., Barringer, K. J., Thill, G. P., and, Stillman, C. A., (1989) Mol. Cell. Biol. 9, 1316-1323.
8. Cregg, J. M., Vedvick, T. S., and, Raschke, W. C., (1993) Nat. Biotechnol. 11, 905-911.
9. Cregg, J. M,., Lin-Cereghino, J., Shi, J., and, Higgins, D.R., (2000) Mol. Biotechnol. 16, 23-52.
10. Laroche, Y., Storme, V., De Meutter, J., Messens, J., and, Lauwereys, M., (1994) Nat. Biotechnol. 12, 1119-1124.
11. Wesolowski, J., Alzogaray, V., Reyelt, J., Unger, M., Juarez, K., Urrutia, M., Cauerhff, A., Danquah, W., Rissiek, B., Sceuplein, F., Schwarz, N., Adriouch, S., Boyer, O., Seman, M., Licea, A., Serreze, D. V., Goldbaum, F. A., Haag, F., and, Koch-Nolte, F., (2009) Med. Microbiol. Immunol. 198, 157-174.
12. Muyldermans, S., Cambillau, C., and, Wyns, L., (2001) Trends Biochem. Sci. 26, 230-235.
13. Omidfar, K., Rasaee, M. J., Modjtahedi, H., Forouzandeh, M., Taghikhani, M., Bakhtiari, A., Paknejad, M., and, Kashanian, S., (2004) Tumor Biol. 25, 179-187.
14. van der Linden, R. H. J., Frenken, L. G. J., Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., Ron, L. D., Wilson, S., Davis, P., and, Verrips, C. T., (1999) Biochim. Biophys. Acta 1431, 37-46.
15. Molloy, P., Brydon, L., Porter, A. J., and, Harris, W. J., (1995) J. Appl. Bacteriol. 78, 359-365.
16. Ashley, N. V., and, Roach, D. J., (1990) J. Chem. Technol. Biotechnol. 49, 381-394.
17. Graham, B. M., Porter, A. J., and, Harris, W. J., (1995) J. Chem. Technol. Biotechnol. 63, 279-289.
18. Got, P. A., and, Scherrmann, J. M., (1997) Pharm. Res. 14, 1516-1523.
19. Janda, K. D., Lo, L. C., Lo, C. H. L., Sim, M. M., Wang, R., Wong, C. H., and, Lerner, R. A., (1997) Science 275, 945-948.
20. Frenken, L. G. J., Hessing, J. G. M., van den Hondel, C. A. M. J. J., and, Verrips, C. T., (1998) Res. Immunol. 149, 589-599.
21. Joosten, V., Lokman, C., Hondel, A. V. D., and, Punt, P. J., (2003) Microbial Cell Factories 2, 1-15.
22. Miranda, F., Kopeyan, C., Rochat, C., Rochat, H., and, Lissitzky, S., (1970) Eur. J. Biochem. 16, 514-523.
23. Rochat, H., Rochat, C., Miranda, F., Lissitzky, S., and, Edman, P., (1970) Eur. J. Biochem. 17, 262-266.
24. Hmila, I., Ben Abderrazek, R., Dirk, S., Benlasfar, Z., Conrath, K., El Ayeb, M., Muyldermans, S., and, Bouhaouala, B., (2008) Mol. Immunol. 45, 3847-3856.
25. Sambrook, J., Fritsch, E. F., and, Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York.
26. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and, Klenk, D. C., (1985) Anal. Biochem. 150, 76-85.
27. Harmsen, M. M., van Solt, C. B., and, Fijten, H. P. D., (2009) Appl. Microbiol. Biotechnol. 84, 1087-1094.
28. Frenken, L. G. J., van der Linden, R. H. J., Hermans, P. W. J. J., Wil Bos, J., Ruuls, R. C., de Geus, B., and, Theo Verrips, C., (2000) J. Biotechnol. 78, 11-21.
29. Muyldermans, S., (2001) Rev. Mol. Biotechnol. 74, 277-302.
30. Harmsen, M. M., and, De Haard, H. J., (2007) Appl. Microbiol. Biotechnol. 77, 13-22.
31. Cereghino, J. L., and, Cregg, J. M., (2000) FEMS Microbiol. Rev. 24, 45-66.
32. Damasceno, L. M., Itzcoatl, P., Chang, H. J., Cohen, L., Ritter, G., Old, L. J., and, Batt, C. A., (2004) Protein Expression Purif. 37, 18-26.
33. Ghahroudi, M., Desmyter, A., Wyns, L., Hamers, W. R., and, Muyldermans, S., (1997) FEBS Lett. 414, 521-526.
34. Omidfar, K., Rasaee, M. J., Modjtahedi, H., Forouzandeh, M., Taghikhani, M., and, Golmakany, N., (2004) Tumor Biol. 25, 296-305.
35. Rahbarizadeh, F., Rasaee, M. J., Forouzandeh, M., and, Allameh, A. A., (2005) Protein Expression Purif. 44, 32-38.
36. Joosten, V., Gouka, R. J., vanden Hondel, C. A., Verrips, C. T., and, Lokman, B. C., (2005) Appl. Microbiol. Biotechnol. 66, 384-392.
37. Nguyen, V. K., Zou, X., Lauwereys, M., Brys, L., Bruggemann, M., and, Muyldermans, S., (2003) B cells. Immunol. 109, 93-101.
38. Rahbarizadeh, F., Rasaee, M. J., Forouzandeh, M., and, Allameh, A., (2006) Pichia pastoris. Mol. Immunol. 43, 426-435.