Differences in responsivity of original cupric reducing antioxidant capacity and cupric-bathocuproine sulfonate assays to antioxidant compounds

Analytical Biochemistry

Volumn 423, Issue 1, 2012, Pages 36-38

  Esin Celik S ^a   Özyürek, Mustafa ^a   Güçlü, Kubilay ^a   Apak, Reşat ^a  

^a ISTANBUL UNIVERSITY   (Turkey)

Author keywords

Bathocuproine sulfonate (BCS); CUPRAC antioxidant assay; Neocuproine (Nc)

Indexed keywords

ACETONE; DICHLOROMETHANE; FLAVONOIDS; PHYSICAL CHEMISTRY;

ANTIOXIDANT ASSAYS; ANTIOXIDANT CAPACITY; ANTIOXIDANT COMPOUNDS; BATHOCUPROINE; BETA CAROTENE; CHELATING LIGANDS; NEOCUPROINE; RESPONSIVITY;

ANTIOXIDANTS;

ACETONE; ALPHA TOCOPHEROL; BATHOCUPROINE SULFONATE; BETA CAROTENE; BILIRUBIN; CHELATING AGENT; DICHLOROMETHANE; NARINGENIN; OLIVE OIL; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; CARROT; CHELATION; CHEMICAL ANALYSIS; FOOD; GRAPEFRUIT; HUMAN; NONHUMAN; PRIORITY JOURNAL; SERUM; URINE;

EID: 84857253362     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.011     Document Type: Article

References (13)

1. R. Apak, K. Güçlü, M. Özyürek, S.E. Karademir, Novel total antioxidant index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, J. Agric. Food Chem. 52 (2004) 7970-7981. (Pubitemid 40053683)
2. M. Özyürek, B. Bektaşoǧlu, K. Güçlü, N. Güngör, R. Apak, Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-b-cyclodextrin using the CUPRAC (cupric reducing antioxidant capacity) method, Anal. Chim. Acta 630 (2008) 28-39.
3. S. Gorinstein, M. Leontowicz, H. Leontowicz, K. Najman, J. Namiesnik, Y.S. Park, S.T. Jung, S.G. Kang, S. Trakhtenberg, Supplementation of garlic lowers lipids and increases antioxidant capacity in plasma of rats, Nutr. Res. 26 (2006) 362-368. (Pubitemid 44279166)
4. C. Campos, R. Guzmán, E. López-Fernandez, A. Casado, Evaluation of the copper(II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: the CUPRAC-BCS assay, Anal. Biochem. 392 (2009) 37-44.
5. G. Da Cruz, Use of bathocuproine for the evaluation of the antioxidant power in liquids and solutions, US patent 6,613,577 (2003).
6. R. Apak, K. Güçlü, M. Özyürek, S.E. Karademir, M. Altun, Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: the CUPRAC method, Free Radic. Res. 39 (2005) 949-961. (Pubitemid 41227031)
7. S.E. Çelik, M. Özyürek, K. Güçlü, R. Apak, Solvent effects on the antioxidant capacity of lipophilic and hydrophilic antioxidants measured by CUPRAC, ABTS/persulphate, and FRAP methods, Talanta 81 (2010) 1300-1309.
8. A.J. Young, G.M. Lowe, Antioxidant and prooxidant properties of carotenoids, Arch. Biochem. Biophys. 385 (2001) 20-27. (Pubitemid 32988978)
9. L. Mueller, V. Böhm, Antioxidant activity of β-carotene compounds in different in vitro assays, Molecules 16 (2011) 1055-1069.
10. I.F.F. Benzie, J.J. Strain, Ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Anal. Biochem. 239 (1996) 70-76.
11. F.M. Pirisi, P. Cabras, C. Falqui Cao, M. Migliorini, M. Muggelli, Phenolic compounds in virgin olive oil: 2. Reappraisal of the extraction, HPLC separation, and quantification procedures, J. Agric. Food Chem. 48 (2000) 1191-1196. (Pubitemid 30243684)
12. X. Ding, H. Xie, Y.J. Kang, The significance of copper chelators in clinical and experimental application, J. Nutr. Biochem. 22 (2011) 301-310.
13. G. Litwinienko, K.U. Ingold, Solvent effects on the rates and mechanisms of reaction of phenols with free radicals, Acc. Chem. Res. 40 (2007) 222-230.