A novel protein refolding protocol for the solubilization and purification of recombinant peptidoglycan-associated lipoprotein from Xylella fastidiosa overexpressed in Escherichia coli

Protein Expression and Purification

Volumn 82, Issue 2, 2012, Pages 284-289

  Santos, Clelton A ^a   Beloti, Lilian L ^a   Toledo, Marcelo A S ^a   Crucello, Aline ^a   Favaro, Marianna T P ^a   Mendes, Juliano S ^a   Santiago, André S ^a   Azzoni, Adriano R ^a,b   Souza, Anete P ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Outer membrane protein; Pal; Peptidoglycan; Protein refolding; Xylella fastidiosa

Indexed keywords

BACTERIAL PROTEIN; LIPOPROTEIN; PEPTIDOGLYCAN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; PROTEIN REFOLDING; PROTEIN SECONDARY STRUCTURE; SEQUENCE HOMOLOGY; SOLUBILITY; XYLELLA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CHROMATOGRAPHY, GEL; ESCHERICHIA COLI; LIPOPROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDOGLYCAN; PROTEIN BINDING; PROTEIN REFOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; SEQUENCE HOMOLOGY, AMINO ACID; SOLUBILITY; XYLELLA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; XYLELLA FASTIDIOSA;

EID: 84857127405     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.01.010     Document Type: Article

References (31)

1. R. Godlewska, K. Wiśniewska, Z. Pietras, and E.K. Jagusztyn-Krynicka Peptidoglycan-associated lipoprotein (Pal) of Gram-negative bacteria: function, structure, role in pathogenesis and potential application in immunoprophylaxis FEMS Microbiol. Lett. 298 2009 1 11
2. L.M. Parsons, F. Lin, and J. Orban Peptidoglycan recognition by Pal, an outer membrane lipoprotein Biochemistry 45 2006 2122 2128
3. J.C. Lazzaroni, and R. Portalier The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL) Mol. Microbiol. 6 1992 735 742
4. E. Cascales, A. Bernadac, M. Gavioli, J.C. Lazzaroni, and R. Lloubes Pal lipoprotein of Escherichia coli plays a major role in outer membrane integrity J. Bacteriol. 184 2002 754 759
5. J. Hellman, J.D. Roberts Jr., M.M. Tehan, J.E. Allaire, and H.S. Warren Bacterial peptidoglycan-associated lipoprotein is released into the bloodstream in gram-negative sepsis and causes inflammation and death in mice J. Biol. Chem. 277 2002 14274 14280
6. M.D. Liang, A. Bagchi, H.S. Warren, M.M. Tehan, J.A. Trigilio, L.K. Beasley-Topliffe, B.L. Tesini, J.C. Lazzaroni, M.J. Fenton, and J. Hellman Bacterial peptidoglycan-associated lipoprotein: a naturally occurring toll-like receptor 2 agonist that is shed into serum and has synergy with lipopolysaccharide J. Infect. Dis. 191 2005 939 948
7. R. Ihalin, M. Karched, K. Eneslatt, and S. Asikainen Characterization of immunoaffinity purified peptidoglycan-associated lipoprotein of Actinobacillus actinomycetemcomitans J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 831 2006 116 125
8. K.R. Fortney, R.S. Young, M.E. Bauer, B.P. Katz, A.F. Hood, R.S. Munson Jr., and S.M. Spinola Expression of peptidoglycan-associated lipoprotein is required for virulence in the human model of Haemophilus ducreyi infection Infect. Immun. 68 2000 6441 6448
9. J.F. Dubuisson, A. Vianney, N. Hugouvieux-Cotte-Pattat, and J.C. Lazzaroni Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence Microbiology 151 2005 3337 3347
10. A.J. Heilpern, and M.K. Waldor CTXphi infection of Vibrio cholerae requires the tolQRA gene products J. Bacteriol. 182 2000 1739 1747
11. Y.C. Yeh, L.R. Comolli, K.H. Downing, L. Shapiro, and H.H. McAdams The Caulobacter Tol-Pal complex is essential for outer membrane integrity and the positioning of a polar localization factor J. Bacteriol. 192 2010 4847 4858
12. M. McMahon, T.F. Murphy, J. Kyd, and Y. Thanavala Role of an immunodominant T cell epitope of the P6 protein of nontypeable Haemophilus influenzae in murine protective immunity Vaccine 23 2005 3590 3596
13. T.F. Murphy, C. Kirkham, and A.J. Lesse Construction of a mutant and characterization of the role of the vaccine antigen P6 in outer membrane integrity of nontypeable Haemophilus influenzae Infect. Immun. 74 2006 5169 5176
14. M.C. Ray, P. Germon, A. Vianney, R. Portalier, and J.C. Lazzaroni Identification by genetic suppression of Escherichia coli TolB residues important for TolB-Pal interaction J. Bacteriol. 182 2000 821 824
15. E. Cascales, and R. Lloubes Deletion analyses of the peptidoglycan- associated lipoprotein Pal reveals three independent binding sequences including a TolA box Mol. Microbiol. 51 2004 873 885
16. T. Clavel, P. Germon, A. Vianney, R. Portalier, and J.C. Lazzaroni TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA Mol. Microbiol. 29 1998 359 367
17. G.W. Zlotnick, V.T. Sanfilippo, J.A. Mattler, D.H. Kirkley, R.A. Boykins, and R.C. Seid Jr. Purification and characterization of a peptidoglycan- associated lipoprotein from Haemophilus influenzae J. Biol. Chem. 263 1988 9790 9794
18. E. Bouveret, H. Bénédetti, A. Rigal, E. Loret, and C. Lazdunski In vitro characterization of peptidoglycan-associated lipoprotein (PAL)-peptidoglycan and PAL-TolB interactions J. Bacteriol. 181 1999 6306 6311
19. C.J. Chang, M. Garnier, L. Zreik, V. Rossetti, and J.M. Bové Culture and serological detection of xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain of Xylella fastidiosa Curr. Microbiol. 27 1993 137 142
20. S. Chatterjee, R.P. Almeida, and S. Lindow Living in two worlds: the plant and insect lifestyles of Xylella fastidiosa Annu. Rev. Phytopathol. 46 2008 243 271
21. T. Baba, T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori, Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection, Mol. Syst. Biol. 2 (2006) 2006.0008.
22. H.B. Nicholas, K.B. Jr. Nicholas, D.W. Deerfield, Genedoc: analysis and visualization of genetic variation, EMBnet.news 4 (1997) 1-4.
23. L. Whitmore, and B.A. Wallace DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data Nucleic Acids Res. 32 2004 W668 W673
24. M. Leduc, D. Joseleau-Petit, and L.I. Rothfield Interactions of membrane lipoproteins with the murein sacculus of Escherichia coli as shown by chemical cross-linking studies of intact cells FEMS Microbiol. Lett. 51 1989 11 14
25. S.R. Loftus, D. Walker, M.J. Maté, D.A. Bonsor, R. James, G.R. Moore, and C. Kleanthous Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9 Proc. Natl. Acad. Sci. USA 103 2006 12353 12358
26. D.A. Bonsor, I. Grishkovskaya, E.J. Dodson, and C. Kleanthous Molecular mimicry enables competitive recruitment by a natively disordered protein J. Am. Chem. Soc. 129 2007 4800 4807
27. T. Mizuno A novel peptidoglycan-associated lipoprotein found in the cell envelope of Pseudomonas aeruginosa and Escherichia coli J. Biochem. 86 1979 991 1000
28. O.D. Monera, C.M. Kay, and R.S. Hodges Protein denaturation with guanidine hydrochloride or urea provides a different estimate of stability depending on the contributions of electrostatic interactions Protein Sci. 3 1994 1984 1991
29. T. Mizuno A novel peptidoglycan-associated lipoprotein (PAL) found in the outer membrane of Proteus mirabilis and other Gram-negative bacteria J. Biochem. 89 1981 1039 1049
30. L.J. McGuffin, K. Bryson, and D.T. Jones The PSIPRED protein structure prediction server Bioinformatics 16 2000 404 405
31. H. Lilie, E. Schwarz, and R. Rudolph Advances in refolding of proteins produced in E. coli Curr. Opin. Biotechnol. 9 1998 497 501