Flow cytometric enumeration of Plasmodium berghei-infected red blood cells stained with SYBR Green I

Acta Tropica

Volumn 122, Issue 1, 2012, Pages 113-118

  Somsak, Voravuth ^a,b   Srichairatanakool, Somdet ^b   Yuthavong, Yongyuth ^a   Kamchonwongpaisan, Sumalee ^a   Uthaipibull, Chairat ^a  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b Faculty of Medicine Chiang Mai University   (Thailand)

Author keywords

Flow cytometry; Malaria; Plasmodium berghei; SYBR Green I

Indexed keywords

CHLOROQUINE; CYANINE DYE; PYRIMETHAMINE; SYBR GREEN I; UNCLASSIFIED DRUG;

BIOASSAY; BLOOD; DETECTION METHOD; DRUG DEVELOPMENT; MALARIA; MICROSCOPY; PARASITE; PARASITIC DISEASE;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIMALARIAL ACTIVITY; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DILUTION; DRUG SCREENING; ERYTHROCYTE; FLOW CYTOMETRY; IN VIVO STUDY; MICROSCOPY; MOUSE; NONHUMAN; PARASITEMIA; PLASMODIUM BERGHEI; PLASMODIUM BERGHEI INFECTION;

ANIMALS; ANTIMALARIALS; ERYTHROCYTES; FLOW CYTOMETRY; FLUORESCENT DYES; MICE; ORGANIC CHEMICALS; PARASITEMIA; PARASITOLOGY; PLASMODIUM BERGHEI; STAINING AND LABELING;

ANIMALIA; PLASMODIUM BERGHEI; PLASMODIUM PARASITES; RODENTIA;

EID: 84857121694     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2011.12.010     Document Type: Article

References (21)

1. Bei A.K., Desimone T.M., Badiane A.S., Ahouidi A.D., Dieye T., Ndiaye D., Sarr O., Ndir O., Mboup S., Duraisingh M.T. A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum. Am. J. Hematol. 2010, 85:234-237.
2. Bhakdi S.C., Sratongno P., Chimma P., Rungruang T., Chuncharunee A., Neumann H.P., Malasit P., Pattanapanyasat K. Re-evaluating acridine orange for rapid flow cytometric enumeration of parasitemia in malaria-infected rodents. Cytometry A 2007, 71:662-667.
3. Bland J.M., Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986, 1:307-310.
4. Bland J.M., Altman D.G. Measuring agreement in method comparison studies. Stat. Methods Med. Res. 1999, 8:135-160.
5. Bowers K.M., Bell D., Chiodini P.L., Barnwell J., Incardona S., Yen S., Luchavez J., Watt H. Inter-rater reliability of malaria parasite counts and comparison of methods. Malar. J. 2009, 8:267.
6. Fleischer B. Editorial: 100years ago: Giemsa's solution for staining of plasmodia. Trop. Med. Int. Health 2004, 9:755-756.
7. Hein-Kristensen L., Wiese L., Kurtzhals J.A., Staalsoe T. In-depth validation of acridine orange staining for flow cytometric parasite and reticulocyte enumeration in an experimental model using Plasmodium berghei. Exp. Parasitol. 2009, 123:152-157.
8. Izumiyama S., Omura M., Takasaki T., Ohmae H., Asahi H. Plasmodium falciparum: development and validation of a measure of intraerythrocytic growth using SYBR Green I in a flow cytometer. Exp. Parasitol. 2009, 121:144-150.
9. Jacobberger J.W., Horan P.K., Hare J.D. Cell cycle analysis of asexual stages of erythrocytic malaria parasites. Cell Prolif. 1992, 25:431-445.
10. Janse C.J., van Vianen P.H., Tanke H.J., Mons B., Ponnudurai T., Overdulve J.P. Plasmodium species: flow cytometry and microfluorometry assessments of DNA content and synthesis. Exp. Parasitol. 1987, 64:88-94.
11. Jimenez-Diaz M.B., Mulet T., Gomez V., Viera S., Alvarez A., Garuti H., Vazquez Y., Fernandez A., Ibanez J., Jimenez M., Gargallo-Viola D., Angulo-Barturen I. Quantitative measurement of Plasmodium-infected erythrocytes in murine models of malaria by flow cytometry using bidimensional assessment of SYTO-16 fluorescence. Cytometry A 2009, 75:225-235.
12. Jimenez-Diaz M.B., Rullas J., Mulet T., Fernandez L., Bravo C., Gargallo-Viola D., Angulo-Barturen I. Improvement of detection specificity of Plasmodium-infected murine erythrocytes by flow cytometry using autofluorescence and YOYO-1. Cytometry A 2005, 67:27-36.
13. Jouin H., Daher W., Khalife J., Ricard I., Puijalon O.M., Capron M., Dive D. Double staining of Plasmodium falciparum nucleic acids with hydroethidine and thiazole orange for cell cycle stage analysis by flow cytometry. Cytometry A 2004, 57:34-38.
14. Ludbrook J. Statistical techniques for comparing measurers and methods of measurement: a critical review. Clin. Exp. Pharmacol. Physiol. 2002, 29:527-536.
15. Martini J., Gramaglia I., Intaglietta M., van der Heyde H.C. Impairment of functional capillary density but not oxygen delivery in the hamster window chamber during severe experimental malaria. Am. J. Pathol. 2007, 170:505-517.
16. Peters W., Robinson B.L. The chemotherapy of rodent malaria XLVII. Studies on pyronaridine and other Mannich base antimalarials. Ann. Trop. Med. Parasitol. 1992, 86:455-465.
17. Rengarajan K., Cristol S.M., Mehta M., Nickerson J.M. Quantifying DNA concentrations using fluorometry: a comparison of fluorophores. Mol. Vis. 2002, 8:416-421.
18. Sanni L.A., Fonseca L.F., Langhorne J. Mouse models for erythrocytic-stage malaria. Methods Mol. Med. 2002, 72:57-76.
19. Smilkstein M., Sriwilaijaroen N., Kelly J.X., Wilairat P., Riscoe M. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrob. Agents Chemother. 2004, 48:1803-1806.
20. Vitzthum F., Geiger G., Bisswanger H., Brunner H., Bernhagen J. A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system. Anal. Biochem. 1999, 276:59-64.
21. Xie L., Li Q., Johnson J., Zhang J., Milhous W., Kyle D. Development and validation of flow cytometric measurement for parasitaemia using autofluorescence and YOYO-1 in rodent malaria. Parasitology 2007, 134:1151-1162.