Development of a recombinant ELISA using yeast (Pichia pastoris)-expressed polypeptides for detection of antibodies against avian influenza A subtype H5

Journal of Virological Methods

Volumn 180, Issue 1-2, 2012, Pages 18-25

  Shehata, A A ^a,b   Fiebig, P ^a   Sultan, H ^b   Hafez, M ^c   Liebert, U G ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b Menoufia University   (Egypt)

^c Berlin University   (Germany)

Author keywords

Avian influenza; ELISA; H5N1; Pichia pastoris; Recombinant haemagglutinin

Indexed keywords

AVIAN INFLUENZA VACCINE; INFLUENZA VIRUS HEMAGGLUTININ; POLYPEPTIDE; VIRUS ANTIBODY;

AGAR DIFFUSION; ANTIBODY DETECTION; ARTICLE; AVIAN INFLUENZA; AVIAN INFLUENZA VIRUS; BLOOD SAMPLING; BROILER; CONTROLLED STUDY; DUCK; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; HEMAGGLUTINATION INHIBITION TEST; INFLUENZA VIRUS A H5N2; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; CHICKENS; DUCKS; HEMAGGLUTINATION INHIBITION TESTS; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; INFLUENZA A VIRUS, H5N1 SUBTYPE; INFLUENZA A VIRUS, H5N2 SUBTYPE; INFLUENZA IN BIRDS; INFLUENZA VACCINES; PEPTIDES; PICHIA; POULTRY DISEASES; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

ANAS; AVES; GALLUS GALLUS; INFLUENZA A VIRUS; ORTHOMYXOVIRIDAE; PICHIA PASTORIS;

EID: 84857063551     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.12.004     Document Type: Article

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