Circulating endothelial cells and endothelial activation in essential thrombocythemia: Results from CD146+ immunomagnetic enrichment-flow cytometry and soluble E-selectin detection

American Journal of Hematology

Volumn 87, Issue 3, 2012, Pages 319-320

  Belotti, Angelo ^a   Elli, Elena ^a   Speranza, Tiziana ^a   Lanzi, Eraldo ^a   Pioltelli, Pietro ^a   Pogliani, Enrico ^a  

^a SAN GERARDO HOSPITAL   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CD146 ANTIGEN; CD45 ANTIGEN; CELL MARKER; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; HYDROXYUREA;

ANGIOGENESIS; CELL ACTIVATION; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HYPERCOAGULABILITY; IMMUNOMAGNETIC SEPARATION; IMMUNOPATHOGENESIS; LETTER; MULTINUCLEAR CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; THROMBOCYTHEMIA;

ALKYLATING AGENTS; ANTICOAGULANTS; ANTIGENS, CD146; ASPIRIN; DRUG THERAPY, COMBINATION; E-SELECTIN; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; FEMALE; FLOW CYTOMETRY; FOLLOW-UP STUDIES; HUMANS; HYDROXYUREA; IMMUNOMAGNETIC SEPARATION; IMMUNOPHENOTYPING; JANUS KINASE 2; MALE; MIDDLE AGED; MUTATION, MISSENSE; NEOVASCULARIZATION, PATHOLOGIC; THROMBOCYTHEMIA, ESSENTIAL;

EID: 84857063225     PISSN: 03618609     EISSN: 10968652     Source Type: Journal    
DOI: 10.1002/ajh.22264     Document Type: Letter

References (11)

1. Fadini GP, Avogaro A. Cell-based methods for ex vivo evaluation of human endothelial biology. Cardiovasc Res 2010; 87: 12-21.
2. Goon PKY, Lip GYH, Boos CJ, et al. Circulating endothelial cells, endothelial progenitor cells, and endothelial microparticles in cancer. Neoplasia 2006; 2: 79-88.
3. Alonci A, Allegra A, Bellomo G, et al. Evaluation of circulating endothelial cells, VEGF and VEGFR2 serum levels in patients with chronic myeloproliferative diseases. Hematol Oncol 2008; 26: 235-239.
4. Shantsila E, Lip GYH. Circulating endothelial cells in health and disease: How do we best quantify them? J Thromb Haemost 2008; 6: 1021-1024.
5. Widemann A, Sabatier F, Arnaud L, et al. CD146-based immunomagnetic enrichment followed by multiparameter flow cytometry: A new approach to counting circulating endothelial cells. J Thromb Haemost 2008; 6: 869-876.
6. Mancuso P, Burlini A, Pruneri G, et al. Resting and activated endothelial cells are increased in the peripheral blood of cancer patients. Blood 2001; 97: 3658-3661.
7. Beerepoot LV, Mehra N, Vermaat JS, et al. Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients. Ann Oncol 2004; 15: 139-145.
8. Treliński J, Wierzbowska A, Krawczyńska A, et al. Circulating endothelial cells in essential thrombocythemia and polycythemia vera: Correlation with JAK2-V617F mutational status, angiogenic factors and coagulation activation markers. Int J Hematol 2010; 91: 792-798.
9. Barbui T, Barosi G, Birgegard G, et al. Philadelphia-negative classical myeloproliferative neoplasms: Critical concepts and management recommendations from European LeukemiaNet. J Clin Oncol 2011; 29: 761-770.
10. Trappenburg MC, van Schilfgaarde M, Marchetti M, et al. Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombocythemia. Haematologica 2009; 94: 911-918.
11. Baxter EJ, Scott LM, Campbell PJ, et al. Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders. Lancet 2005;365:1054-1061.