Simultaneous isolation of total RNA, DNA, and protein using samples obtained by EBUS-TBNA

Journal of Bronchology and Interventional Pulmonology

Volumn 18, Issue 4, 2011, Pages 301-305

  Nakajima, Takahiro ^a,b,c   Anayama, Takashi ^a   Koike, Terumoto ^a   Waddell, Thomas ^a   Keshavjee, Shaf ^a   Kimura, Hideki ^a,b   Yoshino, Ichiro ^a,c   Yasufuku, Kazuhiro ^a,d  

^a TORONTO GENERAL HOSPITAL   (Canada)

^b Chiba Cancer Center   (Canada)

^c CHIBA UNIVERSITY   (Japan)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

DNA; Endobronchial ultrasound guided transbronchial needle aspiration; Molecular testing; Protein; RNA

Indexed keywords

DNA; PROTEOME; RNA; TRANSCRIPTOME;

ARTICLE; COMPARATIVE STUDY; DNA ISOLATION; ENDOBRONCHIAL ULTRASONOGRAPHY; GENOME; HUMAN; HUMAN TISSUE; LUNG NON SMALL CELL CANCER; MAJOR CLINICAL STUDY; MUTATION; PRIORITY JOURNAL; PROTEIN ISOLATION; RNA ISOLATION; TRANSBRONCHIAL ASPIRATION;

EID: 84856918774     PISSN: 19446586     EISSN: 19488270     Source Type: Journal    
DOI: 10.1097/LBR.0b013e31823302b7     Document Type: Article

References (21)

1. Maemondo M, Inoue A, Kobayashi K, et al. Gefitinib or chemotherapy for non-small-cell lung cancer with mutated EGFR. N Engl J Med. 2010;362:2380-2388.
2. Kwak EL, Bang YJ, Camidge DR, et al. Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer. N Engl J Med. 2010;363:1693-1703.
3. Garber K. ALK. Lung cancer, and personalized therapy: portent of the future? J Natl Cancer Inst. 2010;102:672-667.
4. Hirsch FR, Wynes MW, Gandara DR, et al. The tissue is the issue: personalized medicine for non-small cell lung cancer. Clin Cancer Res. 2010;16:4909-4911.
5. Gu P, Zhao YZ, Jiang LY, et al. Endobronchial ultrasound-guided transbronchial needle aspiration for staging of lung cancer: a systematic review and metaanalysis. Eur J Cancer. 2009;45:1389-1396.
6. Adams K, Shah PL, Edmonds L, et al. Test performance of endobronchial ultrasound and transbronchial needle aspiration biopsy for mediastinal staging in patients with lung cancer: systematic review and metaanalysis. Thorax. 2009;64:757-762.
7. Nakajima T, Yasufuku K, Suzuki M, et al. Assessment of epidermal growth factor receptor mutation by endobronchial ultrasound-guided transbronchial needle aspiration. Chest. 2007;132:597-602. (Pubitemid 47282394)
8. Mohamed S, Yasufuku K, Nakajima T, et al. Analysis of cell cycle-related proteins in mediastinal lymph nodes of patients with N2-NSCLC obtained by EBUS-TBNA: relevance to chemotherapy response. Thorax. 2008;63:642-647. (Pubitemid 351969259)
9. Nakajima T, Yasufuku K, Suzuki M, et al. Assessment of chemosensitivity-related aberrant methylation of nonsmall cell lung cancer by EBUS-TBNA. J Bronchol Intervent Pulmonol. 2009;16:10-14.
10. Sakairi Y, Nakajima T, Yasufuku K, et al. EML4-ALK fusion gene assessment using metastatic lymph node samples obtained by endobronchial ultrasoundguided transbronchial needle aspiration. Clin Cancer Res. 2010;16:4938-4945.
11. Nakajima T, Yasufuku K, Nakagawara A, et al. Multigene mutation analysis of metastatic lymph nodes in non-small cell lung cancer diagnosed by EBUS-TBNA. Chest. 2011. (In press) doi: 10.1378/chest.10-3186.
12. Nakajima T, Yasufuku K, Suzuki M, et al. Thymidylate synthase, dihydropyrimidine dehydrogenase, thymidone phosphorylase, orotate phosphoribosyltransferase mRNA expression in lung cancer metastatic lymph node samples obtained by endobronchial ultrasound-guided transbronchial needle aspiration: a pilot study. Clin Lung Cancer. 2011. (In press) doi:10.1016/j.cllc.2011.06.001.
13. Vallières E, Shepherd FA, Crowley J, et al. The IASLC Lung Cancer Staging Project: proposals regarding the relevance of TNM in the pathologic staging of small cell lung cancer in the forthcoming (seventh) edition of the TNM classification for lung cancer. J Thorac Oncol. 2009;4:1049-1059.
14. Nakajima T, Yasufuku K, Iyoda A, et al. The evaluation of lymph node metastasis by endobronchial ultrasound-guided transbronchial needle aspiration: crucial for selection of surgical candidates with metastatic lung tumors. J Thorac Cardiovasc Surg. 2007;134: 1485-1490. (Pubitemid 350101585)
15. Rusch VW, Asamura H, Watanabe H, et al. The IASLC lung cancer staging project: a proposal for a new international lymph node map in the forthcoming seventh edition of the TNM classification for lung cancer. J Thorac Oncol. 2009;4:568-577.
16. Hummon AB, Lim SR, Difilippantonio MJ, et al. Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage. Biotechniques. 2007;42: 467-470.
17. Tolosa JM, Schjenken JE, Civiti TD, et al. Columnbased method to simultaneously extract DNA, RNA, and proteins from the same sample. Biotechniques. 2007;43:799-804. (Pubitemid 350303587)
18. Triant DA, Whitehead A. Simultaneous extraction of high-quality RNA and DNA from small tissue samples. J Hered. 2009;100:246-250.
19. Florell SR, Coffin CM, Holden JA, et al. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol. 2001; 14:116-128. (Pubitemid 32173395)
20. Keating DT, Malizia AP, Sadlier D, et al. Lung tissue storage: optimizing conditions for future use in molecular research. Exp Lung Res. 2008;34:455-466.
21. Lawson MH, Rassl DM, Cummings NM, et al. Tissue banking of diagnostic lung cancer biopsies for extraction of high quality RNA. J Thorac Oncol. 2010;5:956-963.