Evaluation of proton beam radiation sensitivity of proliferating choroidal endothelial and retinal ganglion cells with clonogenic assay

Cutaneous and Ocular Toxicology

Volumn 31, Issue 1, 2012, Pages 14-19

  Balaiya, Sankarathi ^a   Malyapa, Robert ^a   Hsi, Wen ^a   Murthy, Ravi K ^a   Chalam, Kakarla V ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

AMD; CEC; Clonogenic assay; Proton beam; RGC

Indexed keywords

CRYSTAL VIOLET;

ARTICLE; CELL DIVISION; CELL SURVIVAL; CHOROID; CLONOGENIC ASSAY; COMPARATIVE STUDY; ENDOTHELIUM CELL; PROTON RADIATION; RADIOSENSITIVITY; RETINA GANGLION CELL; SENSITIVITY ANALYSIS;

ANIMALS; CELL LINE; CELL SURVIVAL; CHOROID; CYCLOTRONS; ENDOTHELIAL CELLS; MACACA MULATTA; PROTONS; RATS; RETINAL GANGLION CELLS;

EID: 84856907006     PISSN: 15569527     EISSN: 15569535     Source Type: Journal    
DOI: 10.3109/15569527.2011.594697     Document Type: Article

References (17)

1. rd Chew EY. Age-related macular degeneration. Lancet 2008; 372: 1835-1845.
2. Green WR. Histopathology of age-related macular degeneration. Mol Vis 1999;5:27-36
3. Laser photocoagulation of subfoveal neovascular lesions of age-related macular degeneration. Updated fndings from two clinical trials. Macular Photocoagulation Study Group. Arch Ophthalmol 1993;111:1200-129.
4. Photodynamic therapy of subfoveal choroidal neovascularization in age-related macular degeneration with verteporfn: one-year results of 2 randomized clinical trials-TAP report. Treatment of age-related macular degeneration with photodynamic therapy (TAP) Study Group. Arch Ophthalmol 1999;17: 1329-1345.
5. Isola V, Pece A, Parodi MB. Choroidal ischemia after photodynamic therapy with verteporfn for choroidal neovascularization. Am J Ophthalmol 2006;142:680-683. (Pubitemid 44442046)
6. De Gowin RL, Lewis LJ, Hoak JC, Mueller AL, Gibson DP. Radiosensitivity of human endothelial cells in culture. J Lab Clin Med 1974;84:42-48.
7. Finger PT, Chakravarthy U, Augsburger JJ. Radiotherapy and the treatment of age-related macular degeneration. External beam radiation therapy is efective in the treatment of age-related macular degeneration. Arch Ophthalmol 1998;116:1507-1511.
8. Hart PM, Chakravarthy U, Mackenzie G, Chisholm IH, Bird AC, Stevenson MR et al. Visual outcomes in the subfoveal radiotherapy study: a randomized controlled trial of teletherapy for age-related macular degeneration. Arch Ophthalmol 2002;120:1029-1038. (Pubitemid 34856070)
9. Eter N, Wegener A, Schüller H, Spitznas M. Radiotherapy for age related macular degeneration causes transient lens transparency changes. Br j Ophthalmol 2000;84:757-760. (Pubitemid 30438556)
10. Ciulla TA, Danis RP, Klein SB, Malinovsky VE, Soni PS, Pratt LM et al. Proton therapy for exudative age-related macular degeneration: a randomized, sham-controlled clinical trial. Am j Ophthalmol 2002;134:905-906. (Pubitemid 35425461)
11. Hall EJ. (1994). Radiobiology for the Radiobiologist. Philadelphia: JB Lippincott company, 30-39.
12. Franken NA, Rodermond HM, Stap J, Haveman J, van Bree C. Clonogenic assay of cells in vitro. Nat Protoc 2006;1:2315-2319.
13. Fridrichsen E, Slater J, Loredo L, Oeinck SC, Blacharski PA, Garcia CA et al. Proton beam irradiation of subfoveal choroidal neovascularization in age-related macular degeneration. Invest Ophthalmol Vis Sci 1995; 36(Suppl):224.
14. Bakirtzis G, Briscoe C V, Peddie C, Riches AC. Expression of delayed cell death and DNA repair in human epithelial cell lines following exposure to ultraviolet radiation. Rad Protect Dosi 2000;91:77-80.
15. Rubin DB, Drab EA, Ward WF, Smith LJ, Fowell SM. Enzymatic responses to radiation in cultured vascular endothelial and smooth muscle cells. Radiat Res 1984;99:420-432. (Pubitemid 14050062)
16. Chalam KV, Balaiya S, Malyappa RS, Hsi W, Brar VS, Murthy RK. Evaluation of choroidal endothelial cell proliferation after exposure to varying doses of proton beam radiation. Retina (Philadelphia, Pa) 2011;31:169-176.
17. Wood JPM, Lascaratos G, Bron AJ, Osborne NN. Te infuence of visible light exposure on cultured RGC-5 cells. Mol Vis 2008;14:334-344. (Pubitemid 351279914)