Purification of alginate and feasible production of monoclonal antibodies by the alginate-immobilized hybridoma cells

Journal of Bioscience and Bioengineering

Volumn 113, Issue 2, 2012, Pages 233-238

  Selimoglu, S Mert ^a   Ayyildiz Tamis, Duygu ^a   Gurhan, Ismet Deliloglu ^a   Elibol, Murat ^a  

^a EGE UNIVERSITY   (Turkey)

Author keywords

Alginate; Bioprocess; Hybridoma cells; Immobilization; Monoclonal antibody; Purification

Indexed keywords

BIOPROCESSES; CELL TYPES; HYBRIDOMA CELLS; MONOCLONAL ANTIBODY PRODUCTION; POLYPHENOLS; PURIFICATION METHOD; SERUM CONCENTRATION; SHEDDING LIGHT;

ALGINATE; BIOCOMPATIBILITY; CELLS; CYTOLOGY; MONOCLONAL ANTIBODIES; PHENOLS; PROFITABILITY; PURIFICATION; RADIOACTIVE WASTE VITRIFICATION;

CELL IMMOBILIZATION;

ALGINIC ACID; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; FEASIBILITY STUDY; HYBRIDOMA; HYBRIDOMA CELL CULTURE; MOUSE; NONHUMAN; PURIFICATION;

ALGINATES; ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE, TUMOR; CELLS, IMMOBILIZED; GLUCURONIC ACID; HEXURONIC ACIDS; HYBRIDOMAS; MICE; POLYPHENOLS;

EID: 84856767562     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.09.020     Document Type: Article

References (31)

1. Walsh, G.: Biopharmaceuticals: Biochemistry and Biotechnology, 2nd ed., pp. 409-435. John Wiley & Sons, UK (2003).
2. Papoutsakis E.T. Fluid mechanical damage of animal cells in bioreactors. Trends Biotechnol. 1991, 9:427-437.
3. Posillico E.G. Microencapsulation technology for large-scale antibody production. Bio/Technology 1986, 4:114-117.
4. Breguet V., Gugerli R., Stockar U.V., Marison I.W. CHO immobilization in alginate/poly-L-lysine microcapsules: an understanding of potential and limitations. Cytotechnology 2007, 53:81-93.
5. King G., Daugulis A., Faulkner P. Alginate-polylysine microcapsules of controlled membrane molecular weight cutoff for mammalian cells culture engineering. Biotechnol. Prog. 1987, 3:231-241.
6. Griffiths B. Perfusion systems for cell cultivation. Large scale mammalian cell culture technology 1990, 217-250. Marcel Dekker, New York, Basel, Hong Kong. A.S. Lubiniecki (Ed.).
7. Ng A.C., Berry J.M., Butler M. Optimization of physical parameters for cell attachment and growth on macroporous microcarriers. Biotechnol. Bioeng. 1995, 50:627-635.
8. Sinacore M.S., Creswick B.C., Buehler R. Entrapment and growth of murine hybridoma cells in calcium alginate microbeads. Nat. Biotechnol. 1989, 7:1275-1279.
9. Lee G.M., Palsson B.O. Immobilization can improve the stability of hybridoma antibody productivity in serum-free media. Biotechnol. Bioeng. 1990, 36:1049-1055.
10. Lee G.M., Varma A., Palsson B.O. Production of monoclonal antibody using free-suspended and immobilized hybridoma cells: effect of serum. Biotechnol. Bioeng. 1991, 38:821-830.
11. Lee G.M., Kim S.J., Palsson B.O. Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific. Cytotechnology 1994, 16:1-15.
12. Jen A.C., Wake M.C., Mikos A.G. Hydrogels for cell immobilization. Biotechnol. Bioeng. 1996, 50:357-364.
13. Huebner H., Buchholz R. Microencapsulation. Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation 1999, 1785-1798. John Wiley & Sons, USA. M.C. Flickinger, S.W. Drew (Eds.).
14. Orive G., Hernandez R.M., Gascon A.R., Calafiore R., Chang T.M.S., Vos P.D., Hortelano G., Hunkeler D., Lacik I., Pedraz J.L. History, challenges and perspectives of cell microencapsulation. Trends Biotechnol. 2004, 22:87-92.
15. 2 concentration on gelation kinetics. J. Biosci. Bioeng. 1999, 88:686-689.
16. Roscoe J.P., Owsianka A.M. Alginate: a reversible semi-solid medium for investigating cell transformation. Br. J. Cancer 1982, 46:965-974.
17. Kupchik H.Z., Langer R.S., Haberern C., Deriny S.E., Brien M.O. A new method for the three-dimensional in vitro growth of human cancer cells. Exp. Cell Res. 1983, 147:454-460.
18. Arus L., Orive G., Hernandez R., Rodriguez A., Rojas A., Pedraz J.L. The influence of cellular seeding density in the microencapsulation of hybridoma cells. J. Biomater. Sci. Polym. Ed. 2005, 16:521-529.
19. Dusseault J., Tam S.K., Menard M., Polizu S., Jourdan G., Yahia L.H., Halle J.P. Evaluation of alginate purification methods: effect on polyphenol, endotoxin, and protein contamination. Biomed. Mater. Res. Part A 2006, 76:243-251.
20. Braek G.S., Murano E., Paoletti S. Alginate as immobilization material II: determination of polyphenol contaminants by fluorescence spectroscopy, and evaluation methods for their removal. Biotechnol. Bioeng. 1989, 33:90-94.
21. Klöck G., Frank H., Houben R. Production of purified alginates suitable for use in immunoisolated transplantation. Appl. Microbiol. Biotechnol. 1994, 40:638-643.
22. Shirai Y., Hashimoto K., Yamaji H., Tokashiki M. Continuous production of monoclonal antibody with immobilized hybridoma cells in an expanded bed fermenter. Appl. Microbiol. Biotechnol. 1987, 26:495-499.
23. Lee G.M., Palsson B.O. Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI-1640 medium. Biotechnol. Techniques 1990, 4:341-344.
24. Lee G.M., Kaminski M.S., Palsson B.O. Observations consistent with autocrine stimulation of hybridoma cell growth and implications for large-scale antibody production. Biotechnol. Lett. 1992, 14:257-262.
25. Lijakovic I.P., Bugarski D., Plavsic M., Bugarski B. Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. Process Biochem. 2007, 42:167-174.
26. Ayyi{dotless}ldi{dotless}z D., Selimoglu M., Elibol M., Deliloglu-Gürhan S.I. Cultivation of calcium-alginate encapsulated myeloma cells in bioreactor. Int. J. Natural Eng. Sci. 2009, 3:48-53.
27. Reuveny S., Velez D., Miller L., Macmillan J.D. Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors. J. Immunol. Methods 1986, 86:61-69.
28. Dean R.C.J., Karkare S.B., Ray N.G., Runstadler P.W.J., Venkatasubramanian K. Large-scale culture of hybridoma and mammalian cells in fluidized bed bioreactors. Ann. NY Acad. Sci. 1987, 506:129-146.
29. Hagedorn J., Kargi F. Production of monoclonal antibodies by hybridoma cells in a flat sheet membrane bioreactor. Biotechnol. Prog. 1990, 6:220-224.
30. Heath C.A., Dilwith R., Belfort G. Methods for increasing monoclonal antibody production in suspension and entrapped cell cultures: biochemical and flow cytometric analysis as a function of medium serum content. J. Biotechnol. 1990, 15:71-90.
31. Selimoglu S.M., Elibol M. Alginate as an immobilization material for MAb production via encapsulated hybridoma cells. Crit. Rev. Biotechnol. 2010, 30:145-159.