Detecting autophagy in arabidopsis roots by membrane-permeable cysteine protease inhibitor E-64d and endocytosis tracer FM4-64

Plant Signaling and Behavior

Volumn 6, Issue 12, 2011, Pages 1946-1949

  Oh Ye, Yuumi ^a   Inoue, Yuko ^b   Moriyasu, Yuji ^a  

^a SAITAMA UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Arabidopsis; Autolysosome autophagosome; Autophagy; Cysteine protease; Endocytosis; Protease inhibitor; Vacuole

Indexed keywords

ALOXISTATIN; DRUG DERIVATIVE; FM 4 64; FM 4-64; LEUCINE; PYRIDINIUM DERIVATIVE; QUATERNARY AMMONIUM DERIVATIVE;

ARABIDOPSIS; ARTICLE; AUTOPHAGY; CELL CULTURE; CELL VACUOLE; CHEMISTRY; CULTURE MEDIUM; CYTOLOGY; ENDOCYTOSIS; LYSOSOME; METABOLISM; METHODOLOGY; PLANT ROOT; STAINING;

ARABIDOPSIS; AUTOPHAGY; CELLS, CULTURED; CULTURE MEDIA; ENDOCYTOSIS; LEUCINE; LYSOSOMES; PLANT ROOTS; PYRIDINIUM COMPOUNDS; QUATERNARY AMMONIUM COMPOUNDS; STAINING AND LABELING; VACUOLES;

ARABIDOPSIS; NICOTIANA TABACUM;

EID: 84856652465     PISSN: 15592316     EISSN: 15592324     Source Type: Journal    
DOI: 10.4161/psb.6.12.18297     Document Type: Article

References (30)

1. Dunn WA Jr. Autophagy and related mechanisms of lysosome-mediated protein degradation. Trends in Cell Biol 1994; 4:139-43.
2. Klionsky DJ, Ohsumi Y. Vacuolar import of proteins and organelles from the cytoplasm. Annu Rev Cell Dev Biol 1999; 15:1-32.
3. Ahlberg J, Glaumann H. Uptake-microautophagy-and degradation of exogenous proteins by isolated rat liver lysosomes: Effects of pH, ATP and inhibitors of proteolysis. Exp Mol Pathol 1985; 42:78-88.
4. Tuttle DL, Dunn WA Jr. Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris. J Cell Sci 1995; 108:25-35.
5. Sakai Y, Koller A, Rangell LK, Keller GA, Subramani S. Peroxisome degradation by microautophagy in Pichia pastoris: identification of specific steps and morphological intermediates. J Cell Biol 1998; 141:625-36.
6. Klionsky DJ, Emr SD. Autophagy as a regulated pathway of cellular degradation. Science 2000; 290:1717-21.
7. Robinson DG, Hinz G. Vacuole biogenesis and protein transport to the plant vacuole: a comparison with the yeast vacuole and the mammlian lysosome. Protoplasma 1997; 197:1-25.
8. Herman EM, Larkins BA. Protein storage bodies and vacuoles. Plant Cell 1999; 11:601-13.
9. Marty F. Plant vacuoles. Plant Cell 1999; 11:587-99.
10. Moriyasu Y, Hillmer S. Autophagy and vacuole formation. In: Robinson DG, Rogers JC, Eds. Vacuolar Compartments, Annu Plant Reviews. Sheffield Academic Press 2000; 5:71-89.
11. Moriyasu Y, Klionsky DJ. Autophagy in plants. In: Klionsky DJ, Ed. Autophagy. Georgetown, TX: Landes Bioscience 2004; 208-15.
12. Bassham DC, Laporte M, Marty F, Moriyasu Y, Ohsumi Y, Olsen LJ, et al. Autophagy in development and stress responses of plants. Autophagy 2006; 2:2-11.
13. Bassham DC. Plant autophagy-more than a starvation response. Curr Opin Plant Biol 2007; 10:587-93.
14. Kominami E, Hashida S, Khairallah EA, Katunuma N. Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin. J Biol Chem 1983; 258:6093-100.
15. Takeshige K, Baba M, Tsuboi S, Noda T, Ohsumi Y. Autophagy in yeast demonstrated with proteinasedeficient mutants and conditions for its induction. J Cell Biol 1992; 119:301-11.
16. Moriyasu Y, Ohsumi Y. Autophagy in tobacco suspension- cultured cells in response to sucrose starvation. Plant Physiol 1996; 111:1233-41.
17. Moriyasu Y, Inoue Y. Use of protease inhibitors for detecting autophagy. In: Klionsky DJ, Ed. Methods in Enzymology, vol 451. Elsevier Inc., Academic Press 2008; 557-80.
18. Takatsuka C, Inoue Y, Higuchi T, Hillmer S, Robinson DG, Moriyasu Y. Autophagy in tobacco BY-2 cells cultured under sucrose starvation conditions: isolation of the autolysosome and its characterization. Plant Cell Physiol 2011; In press.
19. Yano K, Matsui S, Tsuchiya T, Maeshima M, Kutsuna N, Hasegawa S, et al. Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in cultured tobacco cells. Plant Cell Physiol 2004; 45:951-7.
20. Yamada K, Fuji K, Shimada T, Nishimura M, Hara-Nishimura I. Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final stop of the endocytotic pathway. Plant J 2005; 41:888-98.
21. Liou W, Geuze HJ, Geelen MJH, Slot JW. The autophagic and endocytic pathways converge at the nascent autophagic vacuoles. J Cell Biol 1997; 136:61-70.
22. Saito C, Ueda T, Abe H, Wada Y, Kuroiwa T, Hisada A, et al. A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis. Plant J 2002; 29:245-55.
23. Inoue Y, Suzuki T, Hattori M, Yoshimoto K, Ohsumi Y, Moriyasu Y. AtATG genes, homologs of yeast autophagy genes, are involved in constitutive autophagy in Arabidopsis root tip cells. Plant Cell Physiol 2006; 47:1641-52.
24. Yano K, Suzuki T, Moriyasu Y. Constitutive autophagy in plant root cells. Autophagy 2007; 3:360-2.
25. Inoue Y, Moriyasu Y. Autophagy is not a main contributor to the degradation of phospholipids in tobacco cells cultured under sucrose starvation conditions. Plant Cell Physiol 2006; 47:471-80.
26. Marty F. Cytochemical studies on GERL, provacuoles and vacuoles in root meristematic cells of Euphorbia. Proc Natl Acad Sci USA 1978; 75:852-6.
27. Amelunxen F, Heinze U. Zur Entwicklung der Vacuole in Testa-Zellun des Leinsamens [On the development of the vacuole in the testa cells on Linum seeds]. Eur J Cell Biol 1984; 35:343-54.
28. Yano K, Hattori M, Moriyasu Y. A novel type of autophagy occurs together with vacuole genesis in miniprotoplasts prepared from tobacco culture cells. Autophagy 2007; 3:215-21.
29. Yoshimoto K, Hanaoka H, Sato S, Kato T, Tabata S, Noda T, et al. Processing of ATG8a, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy. Plant Cell 2004; 16:2967-83.
30. Kirisako T, Baba M, Ishihara N, Miyazawa K, Ohsumi M, Yoshimori T, et al. Formation process of autophagosome is traced with Apg8/Aut7 in yeast. J Cell Biol 1999; 147:435-46.