Development of hydrolysis probe-based real-time PCR for identification of virulent gene targets of Burkholderia pseudomallei and B. mallei-a retrospective study on archival cases of service members with melioidosis and glanders

Military Medicine

Volumn 177, Issue 2, 2012, Pages 216-221

  Zhang, Binxue ^a   Wear, Douglas J ^b   Kim, Hs ^a   Weina, Peter ^c   Stojadinovic, Alexander ^d   Izadjoo, Mina ^a  

^a HENRY M JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE   (United States)

^b ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^c WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^d WALTER REED ARMY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ARTICLE; AUTOPSY; BACTERIUM IDENTIFICATION; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; GENETICS; GLANDERS; HUMAN; ISOLATION AND PURIFICATION; MELIOIDOSIS; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SOLDIER; UNITED STATES; VALIDATION STUDY;

AUTOPSY; BACTERIAL TYPING TECHNIQUES; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; DNA PRIMERS; GLANDERS; HUMANS; MELIOIDOSIS; MILITARY PERSONNEL; REAL-TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; UNITED STATES;

EID: 84856629548     PISSN: 00264075     EISSN: None     Source Type: Journal    
DOI: 10.7205/MILMED-D-11-00232     Document Type: Article

References (21)

1. White NJ: Melioidosis. Lancet 2003; 361: 1715-22.
2. Jacob F, Turnbull HM, Arkwright JA, Dobrachian GM: A study of chronic glanders in man with report of a case. Br J Dermatol 2006; 33: 39-55.
3. Rainbow L, Hartl CA, Winstanley C: Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei. J Med Microbiol 2002; 51: 374-84.
4. Warawa J, Woods DE: Type III secretion system cluster 3 is required for maximal virulence of Burkholderia pseudomallei in a hamster infection model. FEMS Microbiol Lett 2005; 242: 101-8.
5. Ulrich MP, Norwood DA, Christensen DR, Ulrich RL: Using real-time PCR to specifically detect Burkholderia mallei. J Med Microbiol 2006; 55: 551-9.
6. Novak RT, Glass MB, Gee JE, et al: Development and evaluation of a realtime PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J Clin Microbiol 2006; 44: 85-90.
7. Whitemore A, Krishnaswami CS: An account of the discovery of hitherto undescribed infective disease occurring among the population of Rangoon. (Cross Ref.) Indian Med Gaz 1912; 47: 262-7.
8. Whitmore A: An account of a Glanders-like disease occurring in Rangoon. (Cross Ref.) J Hyg (Lond) 1913; 13: 1-34.
9. Reckseidler SL, DeShazer D, Sokol PA, Woods DE: Detection of bacterial virulence genes by substractive hybridization: identification of cap-sular polysaccharide of Burkholderia pseudomallei as a major virulence determinant. Infect Immun 2001; 69: 34-44.
10. DeShazer D, Waag DM, Fritz DL, Woods DE: Identification of Burkholderia mallei polysaccharide gene cluster by substractive hybridization and demonstration that the encoded capsule is an essential virulence determinant. Microb Pathog 2001; 30: 253-69.
11. Moore RA, Reckseidler-Zenteno S, Kim H, et al: Contribution of gene loss to the pathogenic evolution of Burkholderia pseudomallei and Burkholderia mallei. Infect Immun 2004; 72: 4172-87.
12. Thibault FM, Valade E, Vidal DR: Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. J Clin Microbiol 2004; 42: 5871-4.
13. Gee JE, Sacchi CT, Glass MB, et al: Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 2003; 41: 4647-54.
14. Godoy D, Randle G, Simpson AJ, et al: Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 2003; 41: 2068-79.
15. Weyant RS, Moss CW, Weaver DG, et al: Identification of unusual pathogenic gram-negative aerobic and facultatively anaerobic bacteria, Ed 2, pp 486-7. Baltimore, MD, Williams & Wilkins, 1995.
16. Winstanley C, Hales BA, Hart CA: Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. J Med Microbiol 1999; 48: 649-56.
17. Chantratita N, Meumann E, Thanwisai A, et al: Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis. J Clin Microbiol 2008; 46: 568-73.
18. Wattiau P, Van Hessche M, Neubauer H, et al: Identificaiton of Burkholderia pseudomallei and related bacteria by multiple-locus sequence typing-derived PCR and real-time PCR. J Clin Microbiol 2007; 45: 1045-8.
19. Siwoski A, Ishkanian A, Garnis C, Zhang L, Rosin M, Lam WL: An efficient method for the assessment of DNA quality of archival microdis-sected specimens. Mod Pathol 2002; 15: 889-92.
20. Zhang B, Izadjoo M, Horkayne-Szakaly I, Morrison A, Wear DJ: Medulloblastoma and Brucellosis-Molecular evidence of Brucella sp in association with central nervous system cancer. J Cancer 2011; 2: 136-41.
21. Van Beers EH, Joosse SA, Ligtenberg MJ, et al: A multiplex PCR predictor for aCGH success of FFPE samples. Br J Cancer 2006; 94: 333-7.