A secreted placental alkaline phosphatase-based reporter assay system for screening of compounds acting at an octopamine receptor stably expressed in a mammalian cell line

Bioscience, Biotechnology and Biochemistry

Volumn 76, Issue 1, 2012, Pages 209-211

  Ohta, Hiroto ^a   Oshiumi, Hiroshi ^a   Hayashi, Naotaka ^b   Imai, Tetsuya ^b   Ozoe, Yoshihisa ^c   Morimura, Shigeru ^a   Kida, Kenji ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

^b Otsuka AgriTechno Co Ltd   (Japan)

^c SHIMANE UNIVERSITY   (Japan)

Author keywords

CAMP; Insecticide; Octopamine receptor; Reporter assay; Secreted placental alkaline phosphatase (SEAP)

Indexed keywords

ALKALINE PHOSPHATASE; CAMP; CAMP-RESPONSE ELEMENTS; HIGH-THROUGHPUT SCREENING; MAMMALIAN CELL LINES; OCTOPAMINE RECEPTOR; REPORTER ASSAY; REPORTER GENE ASSAYS;

CELL CULTURE; GENES; INSECT CONTROL; INSECTICIDES;

PHOSPHATASES;

ALKALINE PHOSPHATASE; INSECTICIDE; NORSYNEPHRINE RECEPTOR; RECEPTOR;

ARTICLE; CELL STRAIN HEK293; DRUG ANTAGONISM; DRUG POTENTIATION; DRUG SCREENING; ENZYMOLOGY; FEMALE; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PLACENTA; PREGNANCY; REPORTER GENE; SECRETION;

ALKALINE PHOSPHATASE; DRUG EVALUATION, PRECLINICAL; FEMALE; GENE EXPRESSION; GENES, REPORTER; HEK293 CELLS; HUMANS; INSECTICIDES; PLACENTA; PREGNANCY; RECEPTORS, BIOGENIC AMINE;

BOMBYX MORI; MAMMALIA;

EID: 84856619548     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.110690     Document Type: Article

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