Rehydration driven RNAi: A novel approach for effectively delivering dsRNA to mosquito larvae

Journal of Medical Entomology

Volumn 49, Issue 1, 2012, Pages 215-218

  Lopez Martinez, Giancarlo ^a   Meuti, Megan ^a   Denlinger, David L ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Culex pipiens; gene silencing; heat shock protein; RNA interference

Indexed keywords

DOUBLE STRANDED RNA; HEAT SHOCK PROTEIN 90; WATER;

ANIMAL; ARTICLE; CULEX; GENETIC TRANSFECTION; GENETICS; LARVA; METABOLISM; METHODOLOGY; RNA INTERFERENCE;

ANIMALS; CULEX; HSP90 HEAT-SHOCK PROTEINS; LARVA; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; TRANSFECTION; WATER;

CULEX PIPIENS;

EID: 84856601542     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1603/ME11122     Document Type: Article

References (17)

1. Bellés, X. 2010. Beyond Drosophila: RNAi in vivo and functional genomics in insects. Annu. Rev. Entomol. 55: 111-28.
2. Benoit, J. B., G. Lopez-Martinez, Z. P. Phillips, K. R. Patrick, and D. L. Denlinger. 2010. Heat shock proteins contribute to mosquito dehydration tolerance. J. Insect Physiol. 56: 151-156.
3. Benoit, J. B., G. López-Martínez, K. R. Patrick, Z. P. Phillips, T. B. Krause, and D. L. Denlinger. 2011. Drinking a hot blood meal elicits a protective heat shock response in mosquitoes. Proc. Natl. Acad. Sci. U.S.A. 108: 8026-8029.
4. Blitzer, E. J., I. Vyazunova, and Q. Lan. 2005. Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti. Insect Mol. Biol. 14: 301-307. (Pubitemid 40801938)
5. Cancino-Rodezno, A., C. Alexander, R. Villaseñor, S. Pacheco, H. Porta, Y. Pauchet, M. Soberón, S. S. Gill, and A. Bravo. 2010. The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis. Insect Biochem. Mol. Biol. 40: 58-63.
6. Guo, S., and K. J. Kemphues. 1995. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81: 611-20.
7. How, S. E., B. Yingyongnarongkul, M. A. Fara, J. J. Díaz- Mochón, S. Mittoo, and M. Bradley. 2004. Polyplexes and lipoplexes for mammalian gene delivery: from traditional to microarray screening. Comb. Chem. High. T. Scr. 7: 423-30. (Pubitemid 39108264)
8. Huvenne, H., and G. Smagghe. 2010. Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. J. Insect Physiol. 56: 227-235.
9. Kim, M. J., and D. L. Denlinger. 2010. RNA interference directed against ribosomal protein S3a suggests a link between this gene and arrested ovarian development during adult diapause in Culex pipiens. Insect Mol. Biol. 19: 27-33.
10. Magalhaes, T., D. E. Brackney, J. C. Beier, and B. D. Foy. 2008. Silencing an Anopheles gambiae catalase and sulfhydryl oxidase increases mosquito mortality after a blood meal. Arch. Insect Biochem. Physiol. 68: 134-143.
11. Patrick, M. L., and T. J. Bradley. 2000. The physiology of salinity tolerance in larvae of two species of Culex mosquitoes: the role of compatible solutes. J. Exp. Biol. 203: 821-830. (Pubitemid 30144450)
12. Peng, R., V. I. Maklokova, J. H. Chandrasshekhar, and Q. Lan. 2011. In vivo functional genomics studies of sterol carrier protein-2 gene in the yellow fever mosquito. PlosOne 6: e18030.
13. Robich, R. M., and D. L. Denlinger. 2005. Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony. Proc. Natl. Acad. Sci. U.S.A. 102: 15912-15917. (Pubitemid 41552842)
14. Sanchez-Vargas, I., E. A. Travanty, K. M. Keene, A.W.E. Franz, B. J. Beaty, C. D. Blair, and K. E. Olson. 2004. RNA interference, arthropod-borne viruses, and mosquitoes. Virus Res. 102: 65-74. (Pubitemid 38447251)
15. Sim, C., and D. L. Denlinger. 2008. Insulin signaling and FOXO regulate the overwintering diapause of the mosquito Culex pipiens. Proc. Natl. Acad. Sci. U.S.A. 105: 6777-6781. (Pubitemid 351754583)
16. Zhang, X., J. Zhang, and K. Y. Zhu. 2010. Chitosan/doublestranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambie). Insect Mol. Biol. 19: 683-693.
17. Zhou, X., M. M. Wheeler, F. M. Oi, and M. E. Scharf. 2008. RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA. Insect Biochem. Mol. Biol. 38: 805-815.