Creation of a producent, optimization of expression, and purifi cation of recombinant Yersinia Pseudotuberculosis L-asparaginase

Bulletin of Experimental Biology and Medicine

Volumn 152, Issue 2, 2011, Pages 219-223

  Sidoruk, K V ^a   Pokrovsky, V S ^b   Borisova, A A ^b   Omeljanuk, N M ^b   Aleksandrova, S S ^b   Pokrovskaya, M V ^b   Gladilina, Ju A ^b   Bogush, V G ^a   Sokolov, N N ^b  

^a Institute of Genetics   (Russian Federation)

^b INSTITUTE OF BIOMEDICAL CHEMISTRY   (Russian Federation)

Author keywords

Antitumor enzyme; L asparaginase; Yersinia pseudotuberculosis

Indexed keywords

ASPARAGINASE; BACTERIAL ENZYME; RECOMBINANT ENZYME;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DRUG ISOLATION; DRUG PURIFICATION; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; YERSINIA PSEUDOTUBERCULOSIS;

ASPARAGINASE; ESCHERICHIA COLI; YERSINIA PSEUDOTUBERCULOSIS;

YERSINIA PSEUDOTUBERCULOSIS;

EID: 84856523472     PISSN: 00074888     EISSN: 15738221     Source Type: Journal    
DOI: 10.1007/s10517-011-1493-7     Document Type: Article

References (8)

1. O. Yu. Abakumova, O. V. Podobed, A. A. Borisova, et al., Biomed. Khim., 54, No. 6, 712-719 (2008).
2. Ju. A. Gladilina, N. N. Sokolov, and Yu. V. Krasotkina, Ibid., No. 4, 482-486 (2008).
3. M. V. Pokrovskaya, N. N. Sokolov, V. S. Pokrovsky, et al., A Method for Detection of L-Asparaginase Producer Bacterial Strains. Decision on Granting an Invention Patent of April 7, 2010, in response to application No. 2008122007.
4. D. Cappelletty, L. R. Chiarelli, M. V. Pasquetto, et al., Biochem. Biophys. Res. Commun., 377, No. 4, 1222-1226 (2008).
5. E. F. Hartree, Anal. Biochem., 192, 215-218 (1991).
6. J. Krasotkina, A. A. Borisova, Y. V. Gervaziev, and N. N. Sokolov, Biotechnol. Appl. Biochem., 39, Pt. 2, 215-221 (2004).
7. A. A. Pritsa, K. T. Papazisis, A. H. Kortsaris, et al., Anticancer Drugs, No. 12, 137-142 (2001).
8. R. B. Reinert, L. M. Oberle, S. A. Wek, et al., J. Biol. Chem., 281, No. 42, 31,222-31,233 (2006).