Detection of Legionella by quantitative-polymerase chain reaction (qPCR) for monitoring and risk assessment

BMC Microbiology

Volumn 11, Issue , 2011, Pages

  Krøjgaard, Louise H ^a,b   Krogfelt, Karen A ^a   Albrechtsen, Hans Jørgen ^b   Uldum, Søren A ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CASE REPORT; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; HUMAN; LEGIONELLA; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; MALE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RISK ASSESSMENT; BACTERIAL COUNT; ENVIRONMENTAL MONITORING; GENETICS; ISOLATION AND PURIFICATION; LIMIT OF DETECTION; METHODOLOGY;

LEGIONELLA; LEGIONELLA PNEUMOPHILA;

COLONY COUNT, MICROBIAL; ENVIRONMENTAL MONITORING; LEGIONELLA; LEGIONELLA PNEUMOPHILA; LIMIT OF DETECTION; POLYMERASE CHAIN REACTION; RISK ASSESSMENT;

EID: 84856433195     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-11-254     Document Type: Article

References (19)

1. Legionnaires' Disease prevention and Control. Rosa F, Troy Michigan: Business News Publishing Company 1993
2. Ecological distribution of Legionella pneumophila. Fliermans CB, Cherry WB, Orrison LH, Smith SJ, Tison DL, Pope DH, Appl Environ Microbiol 1981 41 1 9 16 7013702 (Pubitemid 11134835)
3. Legionella and prevention of legionellosis. Bartram J, Chartier Y, Lee JV, Pond K, Surman-Lee S, (editors), World Health Organization 2007
4. Legionnaires disease in Europe 2007-2008. Joseph CA, Ricketts KD, Euro Surveill 2010 15 8
5. A community outbreak of Legionnaires' disease associated with a cooling tower in Vic and Gurb, Catalonia (Spain) in 2005. Ferre MR, Arias C, Oliva JM, Pedrol A, Garcia M, Pellicer T, Roura P, Dominguez A, Eur J Clin Microbiol Infect Dis 2009 28 2 153 159 10.1007/s10096-008-0603-6 18752009
6. Cluster of Legionnaires disease linked to an industrial plant in southeast Norway, June-July 2008. Borgen K, Aaberge L, Werner-Johansen O, Gjosund K, Storsrud B, Haugsten S, Nygard K, Krogh T, Høiby EA, Caugant DA, Kanestrøm A, Simonsen Ø Blystad H, Euro Surveill 2008 13 38
7. A large Legionnaires' disease outbreak in Pamplona, Spain: early detection, rapid control and no case fatality. Castilla J, Barricarte A, Aldaz J, Garcia CM, Ferrer T, Pelaz C, Pineda S, Baladron B, Martin I, Goni B, Aratajo P, Chamorro J, Lameiro F, Torroba L, Dorronsoro L, Martinez-Artola V, Esparza MJ, Gastaminza MA, Fraile P, Aldaz P, Epidemiol Infect 2008 136 6 823 832 17662166 (Pubitemid 351524017)
8. European Guidelines for Control and Prevention of Travel Associated Legionnaires' Disease: the Italian experience. Rota MC, Caporali MG, Massari M, Euro Surveill 2004 9 2
9. ISO 11731-2:2006 Dansk Standard Water quality-Detection and enumeration of Legionella-Part 2: Direct membrane filtration method for waters with low bacterial counts.
10. Cluster of Legionnaires disease in a newly built block of flats, Denmark, December 2. Krojgaard LH, Krogfelt KA, Albrechtsen HJ, Uldum SA, Euro Surveill 2011 16 1
11. Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. Jensen JS, Borre MB, Dohn B, J Clin Microbiol 2003 41 1 261 266 10.1128/JCM.41.1.261-266.2003 12517858 (Pubitemid 36104199)
12. Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods. Bonetta S, Bonetta S, Ferretti E, Balocco F, Carraro E, J Appl Microbiol 2010 108 5 1576 1583 10.1111/j.1365-2672.2009.04553.x 19796090
13. Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Wellinghausen N, Frost C, Marre R, Appl Environ Microbiol 2001 67 9 3985 3993 10.1128/AEM.67.9.3985-3993.2001 11525995 (Pubitemid 33641688)
14. Quantitative Real-Time Legionella PCR for environmental water samples:Data interpretation. Joly P, falconnet P-A, André J, Weill N, Reyrolle M, Vandenesch F, Maurin M, Etienne J, Jarraud S, Appl Environ Microbiol 2006 72 4 2801 2808 10.1128/AEM.72.4.2801-2808.2006 16597985
15. Quantitative detection of Legionella pneumophila in water samples by immunomagnetioc purification and real-time PCR amplification of the dotA gene. Yanez MA, Carrasco.Serrano C, Barberá VM, Catalán V, Appl Environ Microbiol 2005 71 7 3433 3441 10.1128/AEM.71.7.3433-3441.2005 16000746 (Pubitemid 40980505)
16. An international trial of quantitative PCR for monitoring Legionella in artificial water systems. Lee JV, Lai S, Exner M, Lenz J, Gaia V, Casati S, Hartemann P, Luck C, Pangon B, Ricci ML, Scaturro M, Fontana S, Sabria M, Sanchez I, Assaf S, Surman-Lee S, J Appl Microbiol 2011 110 4 1032 1044
17. Control of Legionella pneumophila in a hospital water system by chlorine dioxide. Walker JT, Mackerness CW, Mallon D, Makin T, Williets T, Keevil CW, J Ind Microbiol 1995 15 4 384 390 10.1007/BF01569995 8605076 (Pubitemid 26022311)
18. Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. Yanez MA, Nocker A, Soria-Soria E, Murtula R, Martinez L, Catalan V, J Microbiol Methods 2011 85 2 124 130 10.1016/j.mimet.2011.02.004 21329735
19. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'nuclease PCR. Nogva HK, Drømtorp SM, Nissen H, Rudi K, Biotechniques 2003 34 4 804 808 12703305