Improved mesenchymal stem cells attachment and in vitro cartilage tissue formation on chitosan-modified poly(l-lactide-co-epsilon-caprolactone) scaffold

Tissue Engineering - Part A

Volumn 18, Issue 3-4, 2012, Pages 242-251

  Yang, Zheng ^a,b   Wu, Yingnan ^b   Li, Chao ^c   Zhang, Tianting ^b   Zou, Yu ^b   Hui, James H P ^a,b   Ge, Zigang ^c   Lee, Eng Hin ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BEARINGS (MACHINE PARTS); BIOCHEMISTRY; CARTILAGE; CELL CULTURE; CHITOSAN; PHYSIOLOGICAL MODELS; POLYMERASE CHAIN REACTION; PROTEINS; STEM CELLS; TISSUE; UNLOADING;

ARTICULAR CARTILAGES; BIOCHEMICAL ASSAY; CAPROLACTONE; CARTILAGE EXTRACELLULAR MATRIXES; CARTILAGE FORMATION; CARTILAGE TISSUE ENGINEERING; CARTILAGE TISSUES; CELL AGGREGATION; CELL COMPATIBILITY; CROSSLINKED; ELASTOMERIC PROPERTIES; F-ACTIN; HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS; IN-VITRO; LOAD-BEARING; MECHANICAL RESPONSE; MESENCHYMAL STEM CELL; MORPHOLOGICAL CHANGES; REAL-TIME PCR; STRESS FIBERS; STRUCTURAL COLLAPSE; YOUNG'S MODULUS;

SCAFFOLDS (BIOLOGY);

BIOLOGICAL MARKER; CHITOSAN; LACTIDE CAPROLACTONE COPOLYMER; LACTIDE-CAPROLACTONE COPOLYMER; POLYESTER; TISSUE SCAFFOLD;

ARTICLE; BIOMECHANICS; CARTILAGE; CELL ADHESION; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SHAPE; CHEMISTRY; CHONDROGENESIS; CYTOLOGY; CYTOSKELETON; DRUG EFFECT; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; MATERIALS TESTING; MESENCHYMAL STEM CELL; METABOLISM; METHODOLOGY; POROSITY; REAL TIME POLYMERASE CHAIN REACTION; TISSUE ENGINEERING; ULTRASTRUCTURE; WETTABILITY; YOUNG MODULUS;

BIOLOGICAL MARKERS; BIOMECHANICS; CARTILAGE; CELL ADHESION; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SHAPE; CHITOSAN; CHONDROGENESIS; CYTOSKELETON; ELASTIC MODULUS; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; HUMANS; MATERIALS TESTING; MESENCHYMAL STEM CELLS; POLYESTERS; POROSITY; REAL-TIME POLYMERASE CHAIN REACTION; TISSUE ENGINEERING; TISSUE SCAFFOLDS; WETTABILITY;

EID: 84856280403     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2011.0315     Document Type: Article

References (40)

1. Wakitani, S., Goto, T., Pineda, S.J., Young, R.G., Mansour, J.M., Caplan, A.I., and Goldberg, V.M. Mesenchymal cellbased repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am 76, 579, 1994.
2. Wakitani, S., Imoto, K., Yamamoto, T., Saito, M., Murata, N., and Yoneda, M. Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis Cartilage 10, 199, 2002.
3. Kuroda, R., Ishida, K., Matsumoto, T., Akisue, T., Fujioka, H., Mizuno, K., Ohgushi, H., Wakitani, S., and Kurosaka, M. Treatment of a full-thickness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells. Osteoarthritis Cartilage 15, 226, 2007.
4. Lee, K.B., Hui, J.H., Song, I.C., Ardany, L., and Lee, E.H. Injectable mesenchymal stem cell therapy for large cartilage defects-a porcine model. Stem Cells 25, 2964, 2007.
5. Mauck, R.L., Yuan, X., and Tuan, R.S. Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. Osteoarthritis Cartilage 14, 179, 2006.
6. Huang, A.H., Stein, A., Tuan, R.S., and Mauck, R.L. Transient exposure to transforming growth factor beta 3 improves the mechanical properties of mesenchymal stem cellladen cartilage constructs in a density-dependent manner. Tissue Eng Part A 15, 3461, 2009.
7. Shao, X.X., Goh, J.C.H., Hutmacher, D.W., and Lee, E.H. Repair of large articular osteochondral defects using hybrid scaffolds and bone marrow derived mesenchymal stem cells in a rabbit model. Tissue Eng 2, 1539, 2006.
8. Ho, S.T., Hutmacher, D.W., Ekaputra, A.K., Hitendra, D., and Hui, J.H. The evaluation of a biphasic osteochondral implant coupled with an electrospun membrane in a large animal model. Tissue Eng Part A 16, 1123, 2010.
9. Chan, B.P., and Leong, K.W. Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J Suppl 4, 467, 2008.
10. Huang, A.H., Farrell, M.J., and Mauck, R.L. Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech 43, 128, 2010.
11. Raghunath, J., Rollo, J., Sales, K.M., Butler, P.E., and Seifalian, A.M. Biomaterials and scaffold design: key to tissue-engineering cartilage. Biotechnol Appl Biochem 46, 73, 2007.
12. Moutos, F.T., Freed, L.E., and Guilak, F. A biomimetic threedimensional woven composite scaffold for functional tissue engineering of cartilage. Nat Mater 6, 162, 2007.
13. Xie, J., Ihara, M., Jung, Y., Kwon, I.K., Kim, S.H., Kim, Y.H., and Matsuda, T. Mechano-active scaffold design based on microporous poly(L-lactide-co-epsilon-caprolactone) for articular cartilage tissue engineering: dependence of porosity on compression force-applied mechanical behaviors. Tissue Eng 12, 449, 2006.
14. Kang, Y., Yang, J., Khan, S., Anissian, L., and Ameer, G.A. A new biodegradable polyester elastomer for cartilage tissue engineering. J Biomed Mater Res A. 77, 331, 2006.
15. Li, C., Wang, L., Yang, Z., Kim, G., Chen, H., and Ge, Z. A viscoelastic chitosan-modified three-dimensional porous poly (L-lactide-co-e-caprolactone) scaffold for cartilage tissue engineering. J of Biomater Sci Polym Ed 23, 405, 2012.
16. Di Martino, A., Sittinger, M., and Risbud, M.V. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 26, 5983, 2005.
17. Neves, S.C., Moreira Teixeira, L.S., Moroni L., Reis, R.L., Van Blitterswijk, C.A., Alves, N.M., Karperien M., and Mano, J.F. Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair. Biomaterials 32, 1068, 2011.
18. Alves da Silva, M.L., Crawford, A., Mundy, J.M., Correlo, V.M., Sol, P., Bhattacharya, M., Hatton, P.V., Reis, R.L., and Neves, N.M. Chitosan/polyester- based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation. Acta Biomater 6, 1149, 2010.
19. Yamamoto, A., Araki, T., Fujimori, K., Yamada, M., Yamaguchi, H., Izumi, K., et al. NaCl-aided Hoechst 33258 staining method for DNA quantification and its application. Histochemistry 92, 65, 1989.
20. Hamid R., Rotshteyn Y., Rabadi L., Parikh R., and Bullock P. Comparison of alamar blue and MTT assays for high through-put screening. Toxicol In Vitro 18, 703, 2004.
21. Mei, Y., Saha, K., Bogatyrev, S.R., Yang, J., Hook, A.L., Kalcioglu, Z.I., et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nat Mater 9, 768, 2010.
22. Ayala, R., Zhang, C., Yang, D., Hwang, Y., Aung, A., Shroff, S.S., et al. Engineering the cell-material interface for controlling stem cell adhesion, migration, and differentiation. Biomaterials 32, 3700, 2011.
23. Woods, A., Wang, G., and Beier, F. Regulation of chondrocyte differentiation by the actin cytoskeleton and adhesive interactions. J Cell Physiol 213, 1, 2007.
24. Kumar, D., and Lassar, A.B. The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol Cell Biol. 29, 4262, 2009.
25. Kim, D.K., Kim, S.J., Kang, S.S., and Jin, E.J. Curcumin inhibits cellular condensation and alters microfilament organization during chondrogenic differentiation of limb bud mesenchymal cells. Exp Mol Med 41, 656, 2009.
26. Kim, D., Kim, J., Kang, S.S., and Jin, E.J. Transforming growth factor-beta3-induced Smad signaling regulates actin reorganization during chondrogenesis of chick leg bud mesenchymal cells. J Cell Biochem 107, 622, 2009.
27. Tuli, R., Tuli, S., Nandi, S., Huang, X., Manner, P.A., Hozack, W.J., Danielson, K.G., Hall, D.J., and Tuan, R.S. Transforming growth factor-beta-mediated chondrogenesis of human mesenchymal progenitor cells involves N-cadherin and mitogen-activated protein kinase and Wnt signaling cross-talk. J Biol Chem 278, 41227, 2003.
28. Hui, T.Y., Cheung, K.M., Cheung, W.L., Chan, D., and Chan, B.P. In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration. Biomaterials 29, 3201, 2008.
29. Jung, Y., Kim, S.H., Kim, Y.H., and Kim, S.H. The effect of hybridization of hydrogels and poly(L-lactide-co-epsilon-caprolactone) scaffolds on cartilage tissue engineering. J Biomater Sci Polym Ed 21, 581, 2010.
30. Jung, Y., Park, M.S., Lee, J.W., Kim, Y.H., Kim, S.H., and Kim, S.H. Cartilage regeneration with highly-elastic threedimensional scaffolds prepared from biodegradable poly(Llactide-co-epsilon-caprolactone). Biomaterials 29, 4630, 2008.
31. Xie, J., Han, Z., Naito, M., Maeyama, A., Kim, S.H., Kim, Y.H., and Matsuda, T. Articular cartilage tissue engineering based on a mechano-active scaffold made of poly(L-lactideco-epsilon-caprolactone): in vivo performance in adult rabbits. J Biomed Mater Res B Appl Biomater 94, 80, 2010.
32. Xie, J., Jung, Y., Kim, S.H., Kim, Y.H., and Matsuda, T. New technique of seeding chondrocytes into microporous poly(Llactide-co-epsilon-caprolactone) sponge by cyclic compression force-induced suction. Tissue Eng 12, 1811, 2006.
33. Ficklin, T., Thomas, G., Barthel, J.C., Asanbaeva, A., Thonar, E.J., Masuda, K., Chen, A.C., Sah, R.L., Davol, A., and Klisch, S.M. Articular cartilage mechanical and biochemical property relations before and after in vitro growth. J Biomech 40, 3607, 2007.
34. Yan, D., Zhou, G., Zhou, X., Liu, W., Zhang, W.J., Luo, X., Zhang, L., Jiang, T., Cui, L., and Cao, Y. The impact of low levels of collagen IX and pyridinoline on the mechanical properties of in vitro engineered cartilage. Biomaterials 30, 814, 2009.
35. Lee, H.J., Yu, C., Chansakul, T., Hwang, N.S., Varghese, S., Yu, S.M., and Elisseeff, J.H. Enhanced chondrogenesis of mesenchymal stem cells in collagen mimetic peptidemediated microenvironment. Tissue Eng Part A 14, 1843, 2008.
36. Liu, S.Q., Tian, Q., Hedrick, J.L., Hui, J.H.P., Ee, P.L., and Yang, Y.Y. Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage. Biomaterials 31, 7298, 2010.
37. Wu, Y.N., Yang, Z., Hui, J.H.P., Ouyang, H.W., and Lee, E.H. Cartilaginous ECM component-modification of the micro-bead culture system for chondrogenic differentiation of mesenchymal stem cells. Biomaterials 28, 4056, 2007.
38. Huang, A.H., Farrell, M.J., Kim, M., and Mauck, R.L. Longterm dynamic loading improves the mechanical properties of chondrogenic mesenchymal stem cell-laden hydrogel. Eur Cell Mater 19, 72, 2010.
39. Jung, Y., Kim, S.H., Kim, S.H., Kim, Y.H., Xie, J., Matsuda, T., and Min, B.G. Cartilaginous tissue formation using a mechano-active scaffold and dynamic compressive stimulation. J Biomater Sci Polym Ed 19, 61, 2008.
40. Jung, Y., Kim, S.H., Kim, Y.H., and Kim, S.H. The effects of dynamic and three-dimensional environments on chondrogenic differentiation of bone marrow stromal cells. Biomed Mater 4, 055009, 2009.