Large-scale expression, purification, and glucose uptake activity of recombinant human FGF21 in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 93, Issue 2, 2012, Pages 613-621

  Zhang, Minjing ^a,c   Jiang, Xuebin ^b   Su, Zhijian ^a,c   Lin, Jiancong ^b   Xiang, Qi ^a   Yang, Zhan ^a   Huang, Yadong ^a   Li, Xiaokun ^c  

^a JINAN UNIVERSITY   (China)

^b GUANGZHOU UNIVERSITY   (China)

^c WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

Expression; Glucose uptake activity; Human fibroblast growth factor 21; Purification

Indexed keywords

ARTIFICIAL GENE; BIOCHEMICAL CHARACTERIZATION; CLINICAL APPLICATION; EXPRESSION; FERMENTORS; GLUCOSE UPTAKE; HUMAN FIBROBLAST; LARGE-SCALE EXPRESSION; LIPID METABOLISMS; METABOLIC DISEASE; NON-ALCOHOLIC FATTY LIVER DISEASE; OPTIMAL CONDITIONS; PEPTIDE SEQUENCES; POTENTIAL DRUG; SEPHADEX; SOLUBLE EXPRESSION; TARGET PROTEINS; TREHALOSE SOLUTION; TYPE-2 DIABETES; WESTERN BLOTS;

AFFINITY CHROMATOGRAPHY; CELL CULTURE; CLONING; ESCHERICHIA COLI; FIBROBLASTS; GENE ENCODING; GLUCOSE; GLUCOSE SENSORS; ION EXCHANGE; METABOLISM; PEPTIDES; PHYSIOLOGY; PURIFICATION;

GLUCOSE OXIDASE;

FIBROBLAST GROWTH FACTOR 21; GLUCOSE OXIDASE; NITRILOTRIACETATE NICKEL; PEROXIDASE; RECOMBINANT HUMAN FIBROBLAST GROWTH FACTOR 21; RESIN; SEPHADEX; TREHALOSE; UNCLASSIFIED DRUG;

BIOCHEMICAL COMPOSITION; CHROMATOGRAPHY; COLIFORM BACTERIUM; DIABETES; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GLUCOSE; HOMEOSTASIS; IMMUNOASSAY; LIPID; OBESITY; OPTIMIZATION; PURIFICATION; RECOMBINATION;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; ASSAY; ESCHERICHIA COLI; GLUCOSE TRANSPORT; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE MAPPING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

BIOREACTORS; BIOTECHNOLOGY; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTORS; GENE EXPRESSION; GENES, SYNTHETIC; GENETIC VECTORS; GLUCOSE; HUMANS; PLASMIDS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84856270372     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3427-8     Document Type: Article

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