Measurement of Neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates

Methods in Molecular Biology

Volumn 844, Issue , 2012, Pages 125-138

  Korkmaz, Brice ^a   Attucci, Sylvie ^a   Epinette, Christophe ^a   Pitois, Elodie ^a   Jourdan, Marie Lise ^b   Juliano, Luiz ^c   Gauthier, Francis ^a  

^a UNIVERSITÉ DE TOURS   (France)

^b CHRU de Tours   (France)

^c FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Fluorogenic substrates; Inflammation; Neutrophil; Serine proteases; Therapeutic inhibitors

Indexed keywords

CATHEPSIN G; FLUORESCENT DYE; LEUKOCYTE ELASTASE; MYELOBLASTIN;

ARTICLE; CYTOLOGY; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NEUTROPHIL; SPECTROFLUOROMETRY; SYNTHESIS;

CATHEPSIN G; ENZYME ACTIVATION; ENZYME ASSAYS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HUMANS; LEUKOCYTE ELASTASE; MYELOBLASTIN; NEUTROPHILS; SPECTROMETRY, FLUORESCENCE;

EID: 84856265447     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-527-5_9     Document Type: Article

References (21)

1. Hedstrom, L. (2002) Serine protease mechanism and specificity, Chem Rev 102, 4501-4523.
2. Heutinck, K. M., ten Berge, I. J., Hack, C. E. et al. (2010) Serine proteases of the human immune system in health and disease, Mol Immunol 47, 1943-1955.
3. Korkmaz, B., Horwitz, M. S., Jenne, D. E. et al. (2010) Neutrophil elastase, proteinase 3, and cathepsin G as therapeutic targets in human diseases, Pharmacol Rev 62, 726-759.
4. Segal, A. W. (2005) How neutrophils kill microbes, Annu Rev Immunol 23, 197-223.
5. Owen, C. A., Campbell, E. J. (1999) The cell biology of leukocyte-mediated proteolysis, J Leukoc Biol 65, 137-150.
6. Campbell, E. J., Campbell M. A., Owen, C. A. (2000) Bioactive proteinase 3 on the cell surface of human neutrophils: quantifi cation, catalytic activity, and susceptibility to inhibition, J Immunol 165, 3366-3374.
7. Owen, C. A., Campbell, M. A., Boukedes, S. S. et al. (1995) Inducible binding of bioactive cathepsin G to the cell surface of neutrophils. A novel mechanism for mediating extracellular catalytic activity of cathepsin G, J Immunol 155, 5803-5810.
8. Owen, C. A., Campbell, M. A., Boukedes, S. S. et al. (1997) Cytokines regulate membranebound leukocyte elastase on neutrophils: a novel mechanism for effector activity, Am J Physiol 272, L385-393.
9. Halbwachs-Mecarelli, L., Bessou, G., Lesavre, P. et al. (1995) Bimodal distribution of proteinase 3 (PR3) surface expression refl ects a constitutive heterogeneity in the polymorphonuclear neutrophil pool, FEBS Lett 374, 29-33.
10. Schreiber, A., Busjahn, A. Luft, F. C. et al. (2003) Membrane expression of proteinase 3 is genetically determined, J Am Soc Nephrol 14, 68-75.
11. Lee, W. L., and Downey, G. P. (2001) Leukocyte elastase: physiological functions and role in acute lung injury, Am J Respir Crit Care Med 164, 896-904.
12. Shapiro, S. D. (2002) Proteinases in chronic obstructive pulmonary disease, Biochem Soc Trans 30, 98-102.
13. Moraes, T. J., Chow, C. W., Downey, G. P. (2003) Proteases and lung injury, Crit Care Med 31, S189-194.
14. Owen, C. A. (2008) Roles for proteinases in the pathogenesis of chronic obstructive pulmonary disease, Int J Chron Obstruct Pulmon Dis 3, 253-268.
15. Horwitz, M., Benson, K. F., Person, R. E. et al. (1999) Mutations in ELA2, encoding neutrophil elastase, defi ne a 21-day biological clock in cyclic haematopoiesis, Nat Genet 23, 433-436.
16. Horwitz, M. S., Duan, Z. Korkmaz, B. et al. (2007) Neutrophil elastase in cyclic and severe congenital neutropenia, Blood 109, 1817-1824.
17. Jenne, D. E., Tschopp, J., Ludemann J. et al. (1990) Wegener's autoantigen decoded, Nature 346, 520.
18. Korkmaz, B., Attucci, S., Jourdan, M. L. et al. (2005) Inhibition of neutrophil elastase by alpha1-protease inhibitor at the surface of human polymorphonuclear neutrophils, J Immunol 175, 3329-3338.
19. Korkmaz, B., Jaillet, J., Jourdan, M. L. et al. (2009) Catalytic activity and inhibition of wegener antigen proteinase 3 on the cell surface of human polymorphonuclear neutrophils. J Biol Chem 284, 19896-19902.
20. Kaiser, E., Colescott, R. L., Bossinger, C. D. et al. (1970) Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides, Anal Biochem 34, 595-598.
21. Coin, I., Beyermann, M., Bienert, M. (2007) Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences, Nat Protoc 2, 3247-3256.