Direct faecal PCR for diagnosis of Shiga-toxin-producing Escherichia coli

The Lancet Infectious Diseases

Volumn 12, Issue 2, 2012, Pages 102-

  Gerritzen, A ^a   Wittke, J W ^a   von Ahsen, N ^a   Wolff, D ^a  

^a Medizinisches Labor Bremen   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

VEROTOXIN 1; VEROTOXIN 2;

BACTERIUM IDENTIFICATION; CELL ASSAY; CYTOTOXICITY TEST; DIAGNOSTIC TEST ACCURACY STUDY; DNA ISOLATION; ENRICHMENT CULTURE; ENZYME IMMUNOASSAY; ESCHERICHIA COLI INFECTION; FECES ANALYSIS; HUMAN; LETTER; MAJOR CLINICAL STUDY; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

DIARRHEA; DISEASE OUTBREAKS; ESCHERICHIA COLI INFECTIONS; FEMALE; HEMOLYTIC-UREMIC SYNDROME; HUMANS; MALE; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84856143137     PISSN: 14733099     EISSN: 14744457     Source Type: Journal    
DOI: 10.1016/S1473-3099(11)70369-3     Document Type: Letter

References (5)

1. Bielaszewska M, Mellmann A, Zhang W, et al. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect Dis 2011, 11:671-676.
2. Frank C, Werber D, Cramer JP, et al. Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany. N Engl J Med 2011, 365:1771-1780. for the HUS Investigation Team.
3. Robert Koch Institute: Eigenschaften des Erregers sowie Hinweise und Hilfestellungen des RKI zur Diagnostik des gegenwärtigen zirkulierenden Ausbruchstamms (accessed May 26, 2011). http://www.rki.de/cln_160/nn_467482/DE/Content/InfAZ/E/EHEC/EHEC__Diagnostik.html.
4. Cebula TA, Payne WL, Feng P Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol 1995, 33:248-250.
5. Gerritzen A Comparison of two enzyme immuno assays and verocell cytotoxicity for detection of verotoxins in human feces. J Lab Med 1998, 22:704-712.