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Volumn 2, Issue 7, 1996, Pages 838-846

Conformation and self-association of a hybrid peptide of cecropin A and melittin with improved antibiotic activity

Author keywords

Aggregation; Antibiotics; Circular dichroism; Helices; Peptides

Indexed keywords

AGGREGATED STRUCTURE; ANTIBIOTIC ACTIVITY; ASSOCIATION CONSTANT; CONCENTRATION DEPENDENCE; ELECTROSPRAY MASS SPECTROMETRY; HELICES; HEXAFLUOROISOPROPANOL; ULTRACENTRIFUGATION;

EID: 84856037778     PISSN: 09476539     EISSN: 15213765     Source Type: Journal    
DOI: 10.1002/chem.19960020717     Document Type: Article
Times cited : (5)

References (49)
  • 1
    • 0025818448 scopus 로고
    • H. G. Boman, Cell 1991, 65, 205-207.
    • (1991) Cell , vol.65 , pp. 205-207
    • Boman, H.G.1
  • 22
    • 84891311098 scopus 로고    scopus 로고
    • note
    • Although a true isodichroic point is expected only for a pure two-state transition, strongly cooperative helix-coil transitions offen give apparent isodichroic points. The pH dependency of the CD spectra of CA(1-7)M(2-9) adds to the complexity of the HFIP-induced transition, as HFIP is very acidic due to the effect of the six fluorine groups, and the pH of the solution varies by ca. one pH unit during the titration.
  • 37
    • 84891328408 scopus 로고    scopus 로고
    • note
    • The lack of concentration dependence of the ultracentrifugation data contrasts with the CD results, which show a considerable variation in the same concentration range. This may be due to the different sensitivities of the two techniques or to small differences in pH between samples, which could cause significant differences in the degree of aggregation, since the pH of 6.5 is very close to that at which the onset of precipitation in 5 mM phosphate and 12% HFIP occurs.
  • 38
    • 84891343287 scopus 로고    scopus 로고
    • note
    • r < 52600.
  • 39
    • 84891285807 scopus 로고    scopus 로고
    • note
    • Samples for ES-MS were unbuffered. As the presence of phosphate tends to increase the degree of aggregation, ES-MS results are expected to be more directly comparable to NMR results, which were also recorded in an unbuffered sample.
  • 42
    • 84891293143 scopus 로고    scopus 로고
    • note
    • If the same packing is maintained between each adjacent molecule in the aggregate, the resulting particle would have the structure of a cholesteric liquid crystal and that might provide an additional explanation for the change in the intensity of the CD spectra of the aggregate as compared to the monomeric helix.
  • 43
    • 84891311525 scopus 로고    scopus 로고
    • note
    • -2, and there is a fourfold decrease in the rate for branched residues [46]. With this reference, even fast components would qualify as protected, in agreement with other NMR observations indicating that this region forms a helical structure.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.