Real-time reverse-transcription polymerase chain reaction: Technical considerations for gene expression analysis

Methods in Molecular Biology

Volumn 817, Issue , 2012, Pages 251-270

  Doak, Shareen H ^a   Zaïr, Zoulikha M ^a  

^a SWANSEA UNIVERSITY   (United Kingdom)

Author keywords

Absolute quantification; Gene expression; Primer validation; Real time RT PCR; Relative quantification; SYBR Green; TaqMan

Indexed keywords

COMPLEMENTARY DNA; FLUORESCENT DYE; MESSENGER RNA; ORGANIC COMPOUND; PRIMER DNA; SYBR GREEN I;

ANIMAL; ARTICLE; EVALUATION; GENE EXPRESSION; GENETICS; HUMAN; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; DNA PRIMERS; DNA, COMPLEMENTARY; FLUORESCENT DYES; GENE EXPRESSION; HUMANS; ORGANIC CHEMICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 84856009980     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-421-6_13     Document Type: Article

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