Analysis of transgene integration and expression following biolistic transfer of different quantities of minimal expression cassette into sugarcane (Saccharum spp. hybrids)

Plant Cell, Tissue and Organ Culture

Volumn 108, Issue 2, 2012, Pages 297-302

  Kim, Jae Yoon ^a   Gallo, Maria ^a   Altpeter, Fredy ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Callus; Indirect embryogenesis; nptII expression cassette; Particle bombardment; Sugarcane (Saccharum spp. hybrids)

Indexed keywords

ANEUPLOIDS; BIOLISTIC TRANSFER; C4 GRASS; CALLUS; EMBRYOGENIC CALLUS; EXPRESSION ANALYSIS; EXPRESSION CASSETTE; GENETIC TRANSFORMATION; GENETICIN; GENOMIC DNA; GOLD PARTICLES; HYBRIDIZATION SIGNAL; INDIRECT EMBRYOGENESIS; INTERSPECIFIC HYBRIDS; PARTICLE BOMBARDMENT; PLASMID DNA; PUTATIVE TRANSGENIC PLANTS; SACCHARUM SPP; SOUTHERN BLOT; SOUTHERN BLOT ANALYSIS; TARGET TISSUES; TRANSGENE; TRANSGENE EXPRESSION; TRANSGENIC LINES; TRANSGENICS;

GENE TRANSFER; GENES; POLYMERASE CHAIN REACTION; SUGARS; TISSUE;

INTEGRATION;

SACCHARUM;

EID: 84855984664     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-011-0043-3     Document Type: Article

References (31)

1. Agharkar M, Lomba P, Altpeter F, Zhang H, Kenworthy K, Lange T (2007) Stable expression of AtGA2ox1 in a low-input turfgrass (Paspalum notatum Flugge) reduces bioactive gibberellin levels and improves turf quality under field conditions. Plant Biotech J 5: 791-801.
2. Altpeter F, Oraby H (2010) Sugarcane. In: Kempken F, Jung C (eds) Biotechnology in agriculture and forestry. Genetic modification of plants. Springer, Heidelberg, pp 453-472.
3. Altpeter F, Vasil V, Srivastava V, Stoger E, Vasil IK (1996) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep 16: 12-17.
4. Altpeter F, Baisakh N, Beachy R, Bock R, Capell T, Christou P, Daniell H, Datta K, Datta S, Dix PJ, Fauquet C, Huang N, Kohli A, Mooibroek H, Nicholson L, Nguyen TT, Nugent G, Raemakers K, Romano A, Somers DA, Stoger E, Taylor N, Visser R (2005) Particle bombardment and the genetic enhancement of crops: myths and realities. Mol Breeding 15: 305-327.
5. Bower R, Birch RG (1992) Transgenic sugarcane plants via microprojectile bombardment. Plant J 2(3): 409-416.
6. Breitler JC, Labeyrie A, Meynard D, Legavre T, Guiderdoni E (2002) Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes. Theor Appl Genet 104: 709-719.
7. Chengalrayan K, Gallo-Meagher M (2001) Effect of various growth regulators on shoot regeneration of sugarcane. In Vitro Cell Dev Biol Plant 37: 434-439.
8. Dixon L, Nyffenegger T, Delley G, Martinez-Izquierdo J, Hohn T (1986) Evidence for replicative recombination in cauliflower mosaic virus. Virology 150: 463-468.
9. Edmé SJ, Comstock JC, Miller JD, Tai PYP (2005) Determination of DNA content and genome size in sugarcane. J Am Soc Sugar Cane Technol 25: 1-16.
10. Falco MC, Tulmann Neto A, Ulian EC (2000) Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane. Plant Cell Rep 19: 1188-1194.
11. FAO (2009) Food and Agriculture Organization of the United Nations publishing FAOSTAT. http://faostat. fao. org/site/567/DesktopDefault. aspx?PageID=567#ancor.
12. Fu X, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sudhakar D, Twyman RM, Christou P, Kohli A (2000) Linear transgene constructs lacking vector backbone sequences generate low copy number transgenic plants with simple integration patterns. Transgenic Res 9: 11-19.
13. Gallo-Meagher M, Irvine JE (1996) Herbicide resistant transgenic sugarcane plants containing the bar gene. Crop Sci 36: 1367-1374.
14. Heinz DJ, Mee GWP, Nickell LG (1969) Chromosome numbers of some saccharum species hybrids and their cell suspension cultures. Amer J Bot 56(4): 450-456.
15. Ingelbrecht IL, Irvine JE, Mirkov E (1999) Posttranscriptional gene silencing in transgenic sugarcane. Dissection of homology-dependent virus resistance in a monocot that has a complex polyploidy genome. Plant Physiol 119: 1187-1197.
16. Jain M, Chengalrayan K, Abouzid A, Gallo M (2007) Prospecting the utility of a PMI/mannose selection system for the recovery of transgenic sugarcane (Saccharum spp. hybrid) plants. Plant Cell Rep 26: 581-590.
17. Jakowitsch J, Papp I, Moscone EA, van der Winden J, Matzke M, Matzke AJM (1999) Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoters in trans. Plant J 17(2): 131-140.
18. James VA, Neibaur I, Altpeter F (2008) Stress inducible expression of the DREB1A transcription factor from xeric, Hordeum spontaneum L. in turf and forage grass (Paspalum notatum Flugge) enhances biotic stress tolerance. Transgenic Res 17: 93-104.
19. Jeffreys AJ, Flavell RA (1977) A physical map of the DNA regions flanking the rabbit β-globin gene. Cell 12: 429-439.
20. Lowe BA, Prakash NS, Way M, Mann MT, Spencer TM, Boddupalli RS (2009) Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA. Transgenic Res 18: 831-840.
21. Menossi M, Silva-Filho MC, Vincentz M, Van-Sluys MA, Souza GM (2008) Sugarcane functional genomics: Gene discovery for agronomic trait development. Int J Plant Genomics (eprint). doi: 10. 1007/s11240-011-9963-1.
22. Müller AE, Kamisugi Y, Grüneberg R, Niedenhof I, Hörold RJ, Meyer P (1999) Palindromic sequences and A+T-rich DNA elements promote illegitimate recombination in Nicotiana tabacum. J Mol Biol 291: 29-46.
23. Odell JT, Nagy F, Chun NH (1985) Identification of DNA sequences required for activity of the cauliflower mosaic virus 35s promoter. Nature 313: 810-812.
24. Rochester DE, Winer JA, Shah DM (1986) The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO J 5: 451-458.
25. Romano A, Raemakers K, Bernardi J, Visser R, Mooibroek H (2003) Transgene organisation in potato after particle bombardment-mediated (co-) transformation using plasmids and gene cassettes. Transgenic Res 12: 461-473.
26. Sandhu S, Altpeter F (2008) Co-integration, co-expression and inheritance of unlinked minimal transgene expression cassettes in an apomictic turf and forage grass (Paspalum notatum Flugge). Plant Cell Rep 27: 1755-1765.
27. Strepp R, Scholz S, Kruse S, Speth V, Reski R (1998) Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin. Proc Natl Acad Sci USA 95: 4368-4373.
28. van Rijs J, Giguère V, Hurst J, van Agthoven T, van Kessel AG, Goyert S, Grosveld F (1985) Chromosomal localization of the human Thy-1 gene. Proc Natl Acad Sci USA 82: 5832-5835.
29. Vidal JR, Kikkert JR, Donzelli BD, Wallace PG, Reisch BI (2006) Biolistic transformation of grapevine using minimal gene cassette technology. Plant Cell Rep 25: 807-814.
30. Weng LX, Deng HH, Xu JL, Li Q, Zhang YQ, Jiang ZD, Li QW, Chen JW, Zhang LH (2011) Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials. Transgenic Res 20: 759-772.
31. Zhao Y, Qian Q, Wang H, Hunang D (2007) Hereditary behavior of bar gene cassette is complex in rice mediated by particle bombardment. J Genet Genomics 34: 824-835.