Improved detection of Trypanosoma brucei by lysis of red blood cells, concentration and LED fluorescence microscopy

Acta Tropica

Volumn 121, Issue 2, 2012, Pages 135-140

  Biéler, Sylvain ^a   Matovu, Enock ^b   Mitashi, Patrick ^c   Ssewannyana, Edward ^d   Bi Shamamba, Stomy Karhemere ^e   Bessell, Paul Richard ^a   Ndung'u, Joseph Mathu ^a  

^a FOUNDATION FOR INNOVATIVE NEW DIAGNOSTICS   (Switzerland)

^b MAKERERE UNIVERSITY   (Uganda)

^c UNIVERSITY OF KINSHASA   (Congo)

^d National Livestock Resources Research Institute NaLIRRI   (Uganda)

^e Institute National de Recherches Biomédicales INRB   (Congo)

Author keywords

Diagnosis; Human African trypanosomiasis; LED fluorescence microscopy; Lysis; Red blood cell; Trypanosome

Indexed keywords

ACRIDINE ORANGE;

BLOOD; BUFFERING; DILUTION; LYSIS; MICROSCOPY; PARASITE PREVALENCE; SEDIMENT; SENSITIVITY ANALYSIS;

AFRICAN TRYPANOSOMIASIS; ANIMAL CELL; ARTICLE; BLOOD SMEAR; BRIGHT FIELD MICROSCOPY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; ERYTHROCYTE LYSIS METHOD; FALSE NEGATIVE RESULT; FEASIBILITY STUDY; FLUORESCENCE MICROSCOPY; GIEMSA STAIN; INTERMETHOD COMPARISON; LIGHT EMITTING DIODE FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PARASITE CONCENTRATION; PARASITE DETECTION; PARASITE EXAMINATION; SEDIMENT; SENSITIVITY ANALYSIS; TRYPANOSOMA BRUCEI;

CELL EXTRACTS; DEMOCRATIC REPUBLIC OF THE CONGO; ERYTHROCYTES; HUMANS; MICROSCOPY, FLUORESCENCE; PARASITOLOGY; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TIME FACTORS; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMIASIS, AFRICAN; UGANDA;

CONGO; UGANDA;

ANIMALIA; TRYPANOSOMA BRUCEI;

EID: 84855963024     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2011.10.016     Document Type: Article

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