Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis

Methods in Molecular Biology

Volumn 825, Issue , 2012, Pages 17-29

  Manku, Gurpreet ^a   Mazer, Monty ^a   Culty, Martine ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

BSA gradient; Nonadherent cell immunocytochemistry; Primary germ cell isolation; Testicular gonocytes

Indexed keywords

ANIMAL; ARTICLE; CELL SEPARATION; CYTOLOGY; DISSECTION; HISTOLOGY; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; MALE; METABOLISM; METHODOLOGY; RAT; SPERMATOGONIUM; SPRAGUE DAWLEY RAT; TESTIS;

ANIMALS; CELL SEPARATION; DISSECTION; IMMUNOHISTOCHEMISTRY; MALE; RATS; RATS, SPRAGUE-DAWLEY; SPERMATOGONIA; TESTIS;

RATTUS;

EID: 84855904863     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-436-0_2     Document Type: Article

References (7)

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5. Culty M (2009) Gonocytes, the forgotten cells of the germ cell lineage. Birth Defects Research Part C 87:1-26
6. Romrell LJ, BellvéAR, Fawcett DW (1976) Separation of mouse spermatogenic cells by sedimentation velocity. A morphological characterization. Dev Biol 49:119-131
7. Thuillier R, Mazer M, Manku G, Boisvert A, Wang Y, Culty M (2010) Interdependence of PDGF and estrogen signaling pathways in inducing neonatal rat testicular gonocytes proliferation. Biology of Reproduction 82:825-836